[1]王杰,周萌,杜伟玲,等.一株东北油豆角枯萎病菌拮抗芽孢杆菌的筛选与鉴定[J].北方园艺,2018,42(04):72-77.[doi:10.11937/bfyy.20172432]
　WANG Jie,ZHOU Meng,DU Weiling,et al.Screening and Identification of One Antagonistic Bacillus Strain Against Fusarium oxysporum in Snap Bean Wilt[J].Northern Horticulture,2018,42(04):72-77.[doi:10.11937/bfyy.20172432]
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一株东北油豆角枯萎病菌拮抗芽孢杆菌的筛选与鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年04 页码: 72-77 栏目: 出版日期: 2018-02-28
Title:
Screening and Identification of One Antagonistic Bacillus Strain Against
Fusarium oxysporum in Snap Bean Wilt
作者:
王杰1; 周萌1; 杜伟玲1; 冯国军2
1.哈尔滨市农业科学院 生物中心，黑龙江 哈尔滨 150029；2.黑龙江大学 农作物研究院，黑龙江 哈尔滨 150080
Author(s):
WANG Jie1; ZHOU Meng1; DU Weiling1; FENG Guojun2
1.Biological Center,Harbin Academy of Agricultural Sciences,Harbin，Heilongjiang 150029;2.Crop Research Institute,Heilongjiang University,Harbin，Heilongjiang 150080
关键词:
油豆角枯萎病; 芽孢杆菌; 甲基营养型芽孢杆菌; 油豆角
Keywords:
snap bean wilt; Bacillus spp.; Bacillus methylotrophicus; snap bean
DOI:
10.11937/bfyy.20172432
文献标志码:
A
摘要:
以东北油豆角枯萎病菌（Fusarium oxysporum f.sp.phaseoli）为靶标菌，采用平板对峙培养和盆栽防效试验方法，从作物根际土壤中筛选对其具有较强拮抗活性的菌株；通过形态学观察及生理生化特性测定，并利用16S rRNA基因、gyrB基因序列分析等方法对拮抗菌株进行了鉴定。结果表明：筛选出的拮抗菌株LZF-5-3为甲基营养型芽孢杆菌（Bacillus methylotrophicus）；在盆栽条件下该菌株对油豆角枯萎病的防效为71.42%。
Abstract:
Fusarium oxysporum f.sp.phaseoli was taken as target bacteria.Bacterial strain was screened from crop rhizosphere soil by using plate confrontation method and potted control efficiency.This strain was identified according to morphological,physiological,biochemical characteristics and the analysis of 16S rRNA gene sequence and gyrB gene sequence.The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against snap bean wilt caused by Fusarium oxysporum f.sp.phaseoli via pot experiments was 71.42%.

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备注/Memo

第一作者简介：王杰（1978-），男，硕士，高级农艺师，研究方向为微生物肥料和微生物农药。E-mail:wangjie.521@163.com.

基金项目：哈尔滨市科技创新人才研究专项资金资助项目（2014RFQYJ070）。
收稿日期：2017-09-26

更新日期/Last Update: 2018-02-24