[1]曾令达,黄郁敏,徐春香,等.淡水沙梨离体叶片愈伤组织诱导技术[J].北方园艺,2017,41(22):62-66.[doi:10.11937/bfyy.20172255]
　ZENG Lingda,HUANG Yumin,XU Chunxiang,et al.Calli Induction Techniques of Danshui Pear Leaf in vitro[J].Northern Horticulture,2017,41(22):62-66.[doi:10.11937/bfyy.20172255]
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淡水沙梨离体叶片愈伤组织诱导技术

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年22 页码: 62-66 栏目: 出版日期: 2017-11-27
Title:
Calli Induction Techniques of Danshui Pear Leaf in vitro
作者:
曾令达1; 2; 黄郁敏1; 徐春香3; 李彩华1
1.惠州学院 生命科学院，广东 惠州 516007；2.惠州学院 生物技术研究所，广东 惠州 516007；3.华南农业大学 园艺学院，广东 广州 510642
Author(s):
ZENG Lingda1; 2; HUANG Yumin1; XU Chunxiang3; LI Caihua1
1.Department of Life Science，Huizhou University,Huizhou,Guangdong 516007;2.Institute of Biotechnology,Huizhou University,Huizhou,Guangdong 516007；3.College of Horticulture,South China Agriculture University,Guangzhou，Guangdong 510642
关键词:
淡水沙梨; 离体叶片; 愈伤组织; 培养基
Keywords:
Danshui pear; leaf in vitro; callus; culture medium
DOI:
10.11937/bfyy.20172255
文献标志码:
A
摘要:
以淡水沙梨离体叶片为外植体，以MS 和White培养基为基本培养基，研究了不同浓度6-BA、NAA等植物生长调节剂及不同接种方式对淡水沙梨继代培养离体叶片的影响。结果表明：MS和White培养基中淡水沙梨的叶片愈伤组织诱导率分别为72.4%和30.9%，叶片最佳愈伤组织诱导配方为MS+6-BA 1.0 mg?L-1+NAA 0.2 mg?L-1，20 d叶龄叶片愈伤组织诱导率为100.0%，40 d叶龄叶片愈伤组织诱导率为60.0%，叶片远轴面平贴愈伤组织诱导率为100.0%，叶片近轴面平贴、叶片直插时愈伤组织诱导率分别为78.3%和50.0%。淡水沙梨离体叶片培养中MS培养基较White利于愈伤组织诱导，叶龄较小进行培养愈伤组织诱导率较高，叶片远轴面平贴的接种方式更适合于叶片的离体培养。
Abstract:
Leaf of Danshui pear was used as explant，MS and White medium as the basic medium,the plant growth regulator 6-BA and NAA and different inoculation methods were studied to obtain callus of Danshui pear.The results showed that leaf callus induction rate of the Danshui pear in MS and White culture medium were 72.4% and 30.9%,the best callus induction formula was MS+6-BA1.0 mg?L-1+NAA 0.2 mg?L-1,20 days leaves the highest callus induction rate was 100.0%,40 days leaves the highest callus induction rate was 60.0%,abaxial surface of flat leaf callus induction rate was 100.0%,the adaxial flat rate of callus induction was 78.3%,when the line of leaf callus induction rate was 50.0%.These results indicate that MS medium was better than White medium for leaf calli induction,younger leaves had higher callus initiation rates,leaf abaxial side in contact with the medium was more suitable for inducing leaf callus in vitro.

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备注/Memo

第一作者简介：曾令达（1974-），男，博士，副教授，研究方向为植物生理生态和生物技术。E-mail：hzlingda@139.com.

基金项目：国家自然科学基金资助项目(31272117)；广东省科技计划资助项目（2013B020304010）；惠州市科技计划资助项目（2013B040009001）；惠州学院自然科学资助项目（C211-0220）。
收稿日期：2017-07-10

更新日期/Last Update: 2017-11-22