[1]刘潮,韩利红,王海波,等.绿豆类甜蛋白家族鉴定与生物信息学分析[J].北方园艺,2017,41(20):31-39.[doi:10.11937/bfyy.20172195]
　LIU Chao,HAN Lihong,WANG Haibo,et al.Identification and Bioinformatics Analysis of Thaumatin-like Protein Family in Vigna radiata[J].Northern Horticulture,2017,41(20):31-39.[doi:10.11937/bfyy.20172195]
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绿豆类甜蛋白家族鉴定与生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 31-39 栏目: 出版日期: 2017-10-30
Title:
Identification and Bioinformatics Analysis of Thaumatin-like Protein Family in Vigna radiata
作者:
刘潮; 韩利红; 王海波; 宋培兵; 唐利洲
曲靖师范学院 云南高原生物资源保护与利用研究中心，生物资源与食品工程学院，云南省高校云贵高原动植物多样性及生态适应性进化重点实验室，云南 曲靖 655011
Author(s):
LIU Chao; HAN Lihong; WANG Haibo; SONG Peibing; TANG Lizhou
Center for Yunnan Plateau Biological Resources Protection and Utilization,Qujing Normal University/College of Biological Resource and Food Engineering/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on YunGui Plateau,Qujing,Yunnan 655011
关键词:
绿豆; 类甜蛋白; 密码子偏性; 生物信息学
Keywords:
Vigna radiata; thaumatin-like protein; codon usage bias; bioinformatics
DOI:
10.11937/bfyy.20172195
文献标志码:
A
摘要:
为了解全基因组水平上绿豆类甜蛋白家族基本生物学特征，通过生物信息学手段，鉴定绿豆类甜蛋白家族成员，并对基因和蛋白结构及染色体定位进行了分析，为进一步克隆绿豆类甜蛋白基因以及探讨其抗真菌活性奠定基础。结果表明：共鉴定出34个绿豆类甜蛋白家族成员，基因主要有4种结构类型，分布在除4号和9号染色体外的其余9条染色体中。该家族蛋白功能主要构成细胞结构组分和参与细胞生物学进程。系统发育分析显示，绿豆类甜蛋白家族归属10个聚类组，其中成员较多的聚类组6和聚类组7中的基因主要来自11号染色体，其中7个基因簇中的基因存在紧密连锁现象，属于旁系同源基因，可能发生了染色体内复制。密码子使用性分析显示，绿豆TLP家族基因偏好A或T作为第三位密码子，但密码子使用总体偏性不强，多数属于低表达基因，多数基因进化受碱基突变和正向选择压力的双重影响。
Abstract:
In order to understand the basic biological characteristics of mungbean thaumatin-like protein (TLP) family on the genomic level,this study analyzed and identified gene structure and phylogenesis of 34 mungbean TLP family members through genome database and bioinformatics method.The results showed that the TLP gene structure classified into four types,TLP genes distributed on nine of eleven chromosomes,phylogenesis analysis showed that mungbean TLP family proteins classified into ten evolutionary groups.Most members of group sixth and seventh mainly derived from chromosome eleven.Analysis revealed mungbean TLP family genes preference A or T as the third codon,but generally codon usage bias was not strong.Most of genes belonged to the low expression genes,and affected by mutation and positive selection pressure.This study laid a foundation for cloning of TLP genes and further functional dissection in antifungal activity.

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备注/Memo

第一作者简介：刘潮(1980-），男，河北景县人，博士，讲师，现主要从事植物病理学等研究工作。E-mail：liuchao@mail.qjnu.edu.cn.

基金项目：国家自然科学基金资助项目(31460179）；云南省高校云南特境内生菌资源的开发与利用科技创新团队资助项目。
收稿日期：2017-07-10

更新日期/Last Update: 2017-10-31