YUAN Yuan,ZHENG Rong,CHUN Lei.AMF Community Structure in Rhizosphere Soil of Amygdalus pedunculata Pall.of Different Slopes in Daqingshan Mountains[J].Northern Horticulture,2017,41(22):102-108.[doi:10.11937/bfyy.20172002]
大青山不同坡位柄扁桃根围土壤丛枝菌根真菌(AMF)群落结构
- Title:
-
AMF Community Structure in Rhizosphere Soil of Amygdalus pedunculata Pall.of Different Slopes in Daqingshan Mountains
- 关键词:
- 柄扁桃; 丛枝菌根真菌(AMF); 不同坡位; 多样性; RDA分析
- Keywords:
- Amygdalus pedunculata Pall.; arbuscular mycorrhizal fungi; different slopes; diversity; RDA analysis
- 文献标志码:
- A
- 摘要:
- 以大青山阳坡柄扁桃幼根及根围土壤为试材,采用Phillips和Hayman的染色、湿筛倾析-蔗糖离心及土壤理化因子常规测定方法,研究了不同坡位柄扁桃根围土壤丛枝菌根真菌(AMF)群落结构及其土壤理化因子,以期了解土壤理化因子对柄扁桃根围土壤AMF群落结构的影响。结果表明:不同坡位柄扁桃菌根侵染率在一定范围内随着海拔的升高呈先升高后降低的现象。在不同坡位柄扁桃根围土壤中共分离出12属33种AMF,其中Funneliformis mosseae和Rhizophagus intraradices属于优势种。下坡位和中坡位AMF的孢子密度显著高于上坡位(P<0.05)。下坡位种的丰度和Shannon-Weiner指数均显著高于中坡位和上坡位(P<0.05)。AMF多样性主要受土壤理化因子全磷、pH、速效钾、速效磷的影响。
- Abstract:
- Rhizosphere soil of Amygdalus pedunculata Pall.of different slopes in Daqingshan Mountains were taken as test materials,the correlation between soil factors and community diversity were evaluated,the method of Phillips and Hayman staining,wet sieve extraction-sucrose centrifugation and soil physical and chemical factors routine determination were adopted.The community structure and soil physical and chemical factors of arbuscular mycorrhizal fungi (AMF) in rhizosphere soil were studied.The results showed that colonization rate at different altitudes increased at first and then decreased with altitude increased in a certain range.33 AMF species were isolated from all soil samples,belonging to 12 genera.F.mosseae and R.intraradicesand were the dominant species.Spore density at downhill and mid-slope were significantly higher than those at the top of slope(P<0.05).The richness and Shannon-Wiener index at down-slope were significantly higher than those at the top of slope andmid-slope(P<0.05).AMF diversity was mainly affected by total phosphorus,pH,available potassium and available phosphorus.
参考文献/References:
[1]王发园,林先贵,周建民.中国AM真菌的生物多样性[J].生态学杂志,2004,23(6):149-154.
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备注/Memo
第一作者简介:圆圆(1993-),女,硕士研究生,研究方向为分子生物学。E-mail:1727909932@qq.com.