[1]虎娟,安韶雅,林哲,等.马蔺DFR基因的克隆及生物信息学特征分析[J].北方园艺,2017,41(24):109-115.[doi:10.11937/bfyy.20171745]
　HU Juan,AN Shaoya,LIN Zhe,et al.Cloning and Bioinformatic Analysis of DFR Gene From Iris lactea Pall.var.chinensis (Fisch.) Koidz[J].Northern Horticulture,2017,41(24):109-115.[doi:10.11937/bfyy.20171745]
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马蔺DFR基因的克隆及生物信息学特征分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 109-115 栏目: 出版日期: 2017-12-25
Title:
Cloning and Bioinformatic Analysis of DFR Gene From Iris lactea Pall.var.chinensis (Fisch.) Koidz
作者:
虎娟1; 2; 安韶雅1; 2; 林哲1; 2; 张虹1; 2; 包晗1; 2; 陈任1; 2
1.宁夏大学 西部特色生物资源保护与利用教育部重点试验室，宁夏 银川 750021;2.宁夏大学 生命科学学院，宁夏 银川 750021
Author(s):
HU Juan1; 2; AN Shaoya1; 2; LIN Zhe1; 2; ZHANG Hong1; 2; BAO Han1; 2; CHEN Ren1; 2
1.Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan,Ningxia 750021;2.School of Life Science,Ningxia University,Yinchuan,Ningxia 750021
关键词:
马蔺; 转录组测序; 花青素; 二羟黄酮醇-4-还原酶; 生物信息学分析
Keywords:
Iris lactea Pall.var.chinensis; transcriptome sequencing; anthocyanin biosynthesis; dihydvroflavonol-4-reductase; bioinformatic analysis
DOI:
10.11937/bfyy.20171745
文献标志码:
A
摘要:
以马蔺花瓣为试材，通过转录组测序，应用PCR技术克隆马蔺花青素生物合成关键酶二羟黄酮醇-4-还原酶（dihydvroflavonol-4-reductase,DFR）基因并获得其生物信息学特征。结果表明：马蔺DFR基因全长1 427 bp，共编码357个氨基酸（该基因命名为IlDFR，GenBank登录号为KY907171）。该基因在氨基酸水平上与多种植物的DFR具有较高的同源性，在系统进化上与同属的荷兰鸢尾（Iris hollandica）的DFR亲缘关系较近；预测其蛋白质相对分子质量为39.99 kDa，等电点为5.89，主要由α-螺旋和β-折叠构成，定位于细胞质，属于酸性亲水不含信号肽的不稳定类蛋白质。马蔺IlDFR有一段氨基酸序列‘VTGASGYVGSWLVMKLLRDGY’与大部分物种的DFR特有相对保守的NADPH结合域非常相似，只有4个氨基酸残基有所差异。
Abstract:
Iris lactea Pall.var.chinensis (Fisch.) Koidz petals were used as material for transcriptome sequencing.According to the annotation data,a key gene encodes dihydvroflavonol-4-reductase (DFR) in its anthocyanin biosynthesis was cloned by using PCR amplification and was charactered using bioinformatic analysis.The full-length of DFR gene of I.lactea var.chinensis consisted of 1 427 bp encoding a protein with 357 amino acid residues (designated as IlDFR,accession No.KY907171).Based on the comparison of the deduced amino acid sequence,IlDFR shared high identity with other plant DFRs,and had high homology with congeneric Iris hollandica DFR.IlDFR had a predicted molecular mass of 39.99 kDa and an isoelectric point of 5.89.It mainly composed of α-helixes and β-sheets,and was considered as an acidic,hydrophilic,no signal peptide and labile protein that located in the cytoplasm.IlDFR had a ‘VTGASGYVGSWLVMKLLRDGY’ amino acid sequence that was similar to the NADPH binding domain which was a typical conserved sequence in most of other plant DFRs,but differenced in 4 amino acid residues.

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备注/Memo

第一作者简介：虎娟（1991-），女，硕士研究生，研究方向为植物分子生理与生物技术。E-mail:380128339@qq.com.

责任作者：陈任（1963-），男，博士，教授，现主要从事植物分子生物学与基因工程等研究工作。E-mail:chenren511@163.com.
基金项目：2013年度宁夏回族自治区科技支撑计划资助项目。
收稿日期：2017-07-13

更新日期/Last Update: 2017-12-29