[1]方华舟,肖习明.不同光照强度对蛹虫草 主要活性成分的影响规律[J].北方园艺,2017,41(24):170-177.[doi:10.11937/bfyy.20171650]
　FANG Huazhou,XIAO Ximing.Influence of Light Intensity on the Key Active Ingredients of Cordyceps militaris[J].Northern Horticulture,2017,41(24):170-177.[doi:10.11937/bfyy.20171650]
点击复制

不同光照强度对蛹虫草 主要活性成分的影响规律

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 170-177 栏目: 出版日期: 2017-12-25
Title:
Influence of Light Intensity on the Key Active Ingredients of Cordyceps militaris
作者:
方华舟1; 肖习明2
1.荆楚理工学院 生物工程学院，湖北 荆门 448000；2.荆门市农业局，湖北 荆门 448000
Author(s):
FANG Huazhou1; XIAO Ximing2
1.Bioengineering College,Jingchu University of Technology,Jingmen,Hubei 448000；2.Jingmen City Bureau of Agriculture,Jingmen,Hubei 448000
关键词:
蛹虫草; 光照强度; 蛋白质; 多糖; 虫草素; 虫草酸
Keywords:
Cordyceps militaris; light intensity; protein; polysaccharides; cordycepin; cordycepic acid
DOI:
10.11937/bfyy.20171650
文献标志码:
A
摘要:
以优质蛹虫草菌株为试材，分别以不同光照强度对蛹虫草发菌、转色、原基分化、子实体生长等阶段进行单因子对照试验，比较不同阶段不同光照强度下的各蛹虫草试验组在蛋白质、多糖、虫草素、虫草酸等主要活性成分含量的差异及特点，探索蛹虫草不同生长阶段的适宜光照强度及规律。结果表明：蛹虫草发菌、转色、原基分化、子实体生长等阶段的光照强度分别约为0~10、500~200、200~500、300~500 lx时，蛹虫草主要活性成分含量相对较高。说明不同生长阶段不同光照强度对蛹虫草主要活性成分的合成和积累可产生明显影响，以各阶段相应适宜光照强度对蛹虫草进行栽培是保证和提高主要活性成分含量的重要措施之一。
Abstract:
Cordyceps militaris was used as test material,the high-quality strain of Cordyceps militaris were invided and the single factor control experiment was carried out at different light intensity on cordyceps militaris,turn,primordium differentiation,fruit body growth and so on.The key ingredients as protein,polysaccharides,cordycepin and cordycepic acid in Cordyceps militaris in the stages of spore germination,coloring,primordium differentiation and sporocarp growth under different light intensity were compared.The results showed that Cordyceps militaris had relatively high-content active ingredients in the stage of spore germination,coloring,primordium differentiation and sporocarp growth,respectively under the light intensity of 0-10 lx,500-200 lx,200-500 lx,300-500 lx.The results showed that Cordyceps militaris in different growth stages could produce more active ingredients under different light intensity.

参考文献/References:

[1]中国科学院中国孢子植物志编辑委员会(梁宗琦主编).中国真菌志(第三十二卷?虫草属)[M].北京:科学出版社,2007.

[2]蒋志涛,戴国梁,潘金火,等.蛹虫草化学成分及药理作用研究进展[J].现代中药研究与实践,2015,29(5):80-83.
[3]孟泽彬,陈林会,韩近雨,等.蛹虫草化学活性成分的研究进展[J].分子植物育种,2015,13(9):2147-2154.
[4]董彩红,李文佳,李增智,等.我国虫草产业发展现状、问题与展望:虫草产业金湖宣言[J].菌物学报,2016,35(1):1-15.
[5]廉家盛,田娇,高日.蛹虫草栽培研究进展及发展前景展望[J].安徽农业科学,2014,42(8):2309-2310.
[6]周思静,刘桂君,尚宏忠,等.蛹虫草人工培养技术研究进展[J].江苏农业科学,2014,42(7):13-17.
[7]方华舟,向会耀,王小艳.不同碳源对蛹虫草菌丝及子实体生长状况的影响[J].荆楚理工学院学报,2010,25(2):5-8.
[8]方华舟,王小艳,向会耀.不同氮源对蛹虫草菌丝及子实体生长状况的影响[J].湖北农业科学,2010,49(11):2734-2737.
[9]李菲.蛹虫草人工高产栽培技术[J].福建农业科技,2015(6):54-56.
[10]左雪枝,朱运英,方华舟.不同光照强度对蛹虫草菌丝及子实体生长的影响[J].北方园艺,2015(16):140-144.
[11]中华人民共和国卫生部.食品中蛋白质的测定:GB5009.5-2010[S].北京:中国标准出版社,2010.
[12]中华人民共和国农业部.食用菌中粗多糖的测定:NY/T1676-2008[S].北京:中国标准出版社,2008.
[13]中华人民共和国农业部.虫草制品中虫草素和腺苷的测定?高效液相色谱法:NY/T2116-2012[S].北京:中国标准出版社,2012.
[14]闫文娟,李泰辉,姜子德.比色法测定广东虫草虫草酸含量[J].食用菌,2010(5):73-74,78.
[15]纪晓鹏,杜双田,房海珍,等.光照度对蛹虫草生长发育的影响[J].西北农业学报,2014,23(11):173-167.
[16]简李茹,纪晓鹏,杜双田.光条件对人工培养蛹虫草子座产量及虫草素和腺苷的影响[J].西北农业学报,2015,24(4):163-179.
[17]郭明敏,杨涛,卜宁,等.大型真菌光受体及其功能研究进展[J].菌物学报,2015,34(5):880-889.
[18]李琴,毛玉梅,付鸣佳,等.蛹虫草中蓝光受体基因〖STBX〗Cmwc-1和Cmwc-2〖STBZ〗的表达特性分析[J].湖南农业大学学报(自然科学版),2016,24(6):601-607.
[19]陈安徽,邵颖,陈尚龙,等.高温和强光对蛹虫草子实体成分和酪氨酸酶抑制活性的影响[J].安徽农业大学学报,2016,43(6):1004-1009.
[20]殷东林,段鸿斌,王锐丽.蛹虫草有性生殖研究[J].北方园艺,2014(3):130-133.
[21]李海洲,陈正余,袁学勤,等.蛹虫草栽培的关键因素及发展趋势[J].安康学院学报,2015,27(5):104-106.

相似文献/References:

[1]隋益虎,张雪平,李慧敏,等.不同设施覆盖类型冬至前后的温度和光照性能比较[J].北方园艺,2013,37(04):47.
[2]张艳艳,张嘉翔,袁晓婷,等.不同光照强度对二苞黄精植株生长发育的影响[J].北方园艺,2013,37(17):61.
　ZHANG Yan-yan,ZHANG Jia-xiang,YUAN Xiao-ting,et al.Effect of Different Illumination Intensities on the Growth and Development of Polygonatum involucratum Maxim[J].Northern Horticulture,2013,37(24):61.
[3]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
　GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(24):148.
[4]孙露露,李焕端,张至远,等.蛹虫草甜米酒的生产工艺研究[J].北方园艺,2014,38(05):125.
　SUN Lu-lu,LI Huan-duan,ZHANG Zhi-yuan,et al.Study on Production Techniques of Cordyceps Militaris Sweet Rice Wine[J].Northern Horticulture,2014,38(24):125.
[5]朱雪荣,张文,李丙智,等.不同修剪量对盛果期苹果树光合能力及果实品质的影响[J].北方园艺,2013,37(15):11.
　ZHU Xue-rong,ZHANG Wen,LI Bing-zhi,et al.Effect of Different Pruning Quantity on Apple Photosynthetic Ability and Fruit Quality in Full Fruit Period[J].Northern Horticulture,2013,37(24):11.
[6]张国珍,曾富春,李策宏,等.不同光照处理对五种观赏蕨生长发育及观赏特性的影响[J].北方园艺,2014,38(09):93.
　ZHANG Guo-zhen,ZENG Fu-chun,LI Ce-hong,et al.Effect of Different Light Treatment on Growth and Ornamental Feature of Five Ornamental Ferns[J].Northern Horticulture,2014,38(24):93.
[7]祁德富,马 琪.不同天气条件下日光温室光照强度及温湿度日变化特征研究[J].北方园艺,2013,37(23):55.
　QI De-fu,MA Qi.Study on Diurnal Variation Characteristics of Light Intensity，Temperature and Humidity in Solar Greenhouse Under Different Weather Conditions[J].Northern Horticulture,2013,37(24):55.
[8]方华舟.蛹虫草菌种培养性状与出草结果关系的研究[J].北方园艺,2013,37(12):161.
　FANG Hua-zhou.Study on the Relationship Between Cultural Characteristics and Results of Weeds of Cordyceps militaris[J].Northern Horticulture,2013,37(24):161.
[9]殷东林,段鸿斌,王锐丽.蛹虫草有性生殖研究[J].北方园艺,2014,38(03):130.
　YIN Dong-lin,DUAN Hong-bin,WANG Rui-li.Study on the Sexual Reproduction of Cordyceps militaris[J].Northern Horticulture,2014,38(24):130.
[10]王晓冬,唐焕伟,曲彦婷.光照强度对郁金香生长发育和内源激素含量的影响[J].北方园艺,2012,36(01):84.
　WANG Xiao-dong,TANG Huan-wei,QU Yan-ting.Effect of Illumination Intensities on Growth and Hormone Contents of Tulipa gesneriana L.[J].Northern Horticulture,2012,36(24):84.
[11]左雪枝,朱运英,方华舟.不同光照强度对蛹虫草菌丝及子实体生长的影响[J].北方园艺,2015,39(16):140.[doi:10.11937/bfyy.201516033]
　ZUO Xuezhi,ZHU Yunying,FANG Huazhou.Effect of Different Light Intensities on the Growth of Hyphal and Sporocarp in Cordyceps militaris[J].Northern Horticulture,2015,39(24):140.[doi:10.11937/bfyy.201516033]

备注/Memo

第一作者简介：方华舟（1965-），男，本科，教授，研究方向为食用菌与农业微生物学。E-mail：434761170@qq.com.

基金项目：湖北省教育厅重点科研资助项目(D 20126101)；湖北省荆门市科学技术局科研资助项目(YFYB2017003)。
收稿日期：2017-07-14

更新日期/Last Update: 2017-12-29