YAO Na,LIU Xiuming,DONG Yuanyuan,et al.Advances in Application and Seguencing Methods of Transcriptome[J].Northern Horticulture,2017,41(12):192-198.[doi:10.11937/bfyy.201712043]
转录组的测序方法及应用研究概述
- Title:
- Advances in Application and Seguencing Methods of Transcriptome
- Keywords:
- transcriptome; sequencing; 454 sequencing; Solexa; SOLiD
- 文献标志码:
- A
- 摘要:
- 转录组研究是基因的结构和功能研究的基础，转录组测序技术在近年来取得了很大的进展，被应用到多种动植物中。利用其能够获得大量未知序列的优势，已经被广泛应用到未知基因挖掘、分子调控及相关代谢途径中。该研究主要介绍了转录组研究的方法、转录组测序技术的发展及其在动植物中的重要应用，并讨论其存在的问题及发展趋势。
- Abstract:
- Transcriptome is the basis of the research of structure and function of genes，transcriptome sequencing technology has made great progress in recent years and been applied to many plants and animals species.It has been widely applied to unknown gene mining，molecular regulation and related metabolic pathways for its lots of known sequence.This study reviewed methods of transcriptome，development of transcriptome sequencing and its application in plants and animals，we discussed the problem of tanscriptome and the development trend in the future.
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备注/Memo
第一作者简介：姚娜(1980-)，女，硕士，实验师，现主要从事生物反应器等研究工作。E-mail:52730603@qq.com.责任作者：李海燕（1971-），女，博士，教授，现主要从事植物抗逆工程与分子生物学等研究工作。E-mail:hyli99@163.com.基金项目：国家自然科学基金资助项目（31501366,31101172）；国家“863”高技术研究发展计划资助项目（2011AA100606）；吉林省科技厅资助项目（20150623024TC-11）；大学生创新创业训练计划资助项目（201510193033）。