ZHOU Yanfang,ZHAO Weiqiang,WANG Yanchun.Pathogen Identification of Tomato Gray Leaf Spot in Chifeng[J].Northern Horticulture,2017,41(22):55-58.[doi:10.11937/bfyy.20171124]
赤峰地区番茄灰叶斑病病原菌的鉴定
- Title:
- Pathogen Identification of Tomato Gray Leaf Spot in Chifeng
- Keywords:
- Chifeng; gray leaf spot of tomato; pathogens; Stemphylium solani
- 文献标志码:
- A
- 摘要:
- 以番茄灰叶斑病为研究对象,采用形态学鉴定、致病性检测和分子生物学等方法,研究了赤峰地区番茄灰叶斑病的病原菌种类,以期为该病害的防治提供参考依据。结果表明:病原菌(ZTWYF16081515)分生孢子淡褐色,长方形至圆柱形,大小为(21.4~71.9)μm×(10.5~27.3)μm。分离菌株喷雾接种,7 d后番茄叶片上形成典型的番茄灰叶斑病病斑。核糖体RNA基因内转录间隔区(ITS)和gpd区的PCR产物经测序后进行BLAST分析,均表明该菌与茄匍柄霉S.solani的ITS和gpd序列100%相同,确定该病原为茄匍柄霉S.solani。
- Abstract:
- Tomato gray leaf spot was used as object,the pathogen of tomato gray leaf spot in Chifeng were studied by morphological,pathogenicity testing and molecular sequence analysis.The results showed that conidia were light brown,rectangle or cylindrical,size was(21.4-71.9)μm×(10.5-27.3)μm.After spraying inoculation with spore suspension,typical spots appeared on the leaves of tomato in 7 days.PCR product were sequnenced after BLAST analysis,rDNA ITS and gpd of ZTWYF16081515 revealed 100% nucleotide identity to that of S.solani.It came into conclusion that the pathogen causing leaf spot of tomato in Chifeng to be identified as S.solani.
参考文献/References:
[1]刘安敏,孙家栋,陶秀珍,等.保护地番茄灰叶斑病的发生与综合防治[J].中国植保导刊,2004(4):23-24.
相似文献/References:
[1]崔聪聪,孟令强,曲宝茹,等.赤峰地区蔬菜工厂化育苗的现状及存在问题分析[J].北方园艺,2014,38(05):173.
CUI Cong-cong,MENG Ling-qiang,QU Bao-ru,et al.Analysis of Present Situation and Problems on Vegetable Industrialization Seedling in Chifeng[J].Northern Horticulture,2014,38(22):173.
备注/Memo
第一作者简介:周艳芳(1986-),女,硕士,助理研究员,现主要从事植物病虫害等研究工作。E-mail:yecaode@126.com.