CHEN Wenzhi,WU Mengxi,LUO Qiao,et al.Effects of Two Surfactants on Growth of Solanum nigrum L.Under Cadmium Stress[J].Northern Horticulture,2017,41(11):1-7.[doi:10.11937/bfyy.201711001]
两种表面活性剂对镉胁迫下龙葵生理特性的影响
- Title:
- Effects of Two Surfactants on Growth of Solanum nigrum L.Under Cadmium Stress
- Keywords:
- cadmium stress; rhamnolipids; saponins; Solanum nigrum L.; growth
- 文献标志码:
- A
- 摘要:
- 以镉污染土壤为试材，以龙葵为研究对象，采用盆栽试验，分别研究了不同浓度鼠李糖脂和皂角苷对镉（Cd）胁迫下龙葵(Solanum nigrum L.)生物量、株高和生理指标的影响。结果表明：在镉胁迫下，随着鼠李糖脂和皂角苷浓度的增加，龙葵的株高、生物量、叶绿素a (chl a)、叶绿素b (chl b)、总叶绿素含量、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性以及可溶性蛋白质含量呈现先增高后降低的趋势，游离脯氨酸、丙二醛(MDA)含量升高，而可溶性糖含量则呈现降低的趋势。在单独施加鼠李糖脂的条件下，较低浓度鼠李糖脂（0.25、0.50 g?L-1）能够提高龙葵植株的株高和生物量，缓解重金属Cd 对龙葵的毒害，并在鼠李糖脂浓度为0.50 g?L-1时，缓解毒害的作用最为明显；而高浓度鼠李糖脂（5.00、7.50 g?L-1）则会加剧对龙葵的毒害。而单独施加皂角苷则在处理浓度≤5.00 g?L-1时促进龙葵的生长，尤以皂角苷浓度为5.00 g?L-1时的效果最好，但效果并不显著；当皂角苷浓度为7.50 g?L-1时，则会抑制龙葵的生长。综合来看，鼠李糖脂的处理效果优于皂角苷。
- Abstract:
- A series of pot experiment was conducted to study the effects of different rhamnolipids and saponins concentrations on biomass，plant height and some physiological and biochemical indexes of Solanum nigrum L.under cadmium stress.The results showed that compared with the control，with increasing of rhamnolipids and saponins concentrations，the plant height，biomass，chlorophyll a，chlorophyll b，total chlorophyll，soluble protein contents and antioxidant enzymes (POD，SOD) activity were first increased and then dropped.While the free proline，malondialdehyde (MDA) contents increased.In addition，the soluble sugar content decreased to some extent.〖JP4〗According to the change of these indexes，lower concentrations of rhamnolipid (0.25 g?L-1，0.50 g?L-1) 〖JP3〗could promote Solanum nigrum L.growth and partly mitigate the toxicity of Cd to Solanum nigrum L.plants，and the effect of the rhamnolipid of 0.50 g?L-1was most obvious.However，high concentrations of rhamnolipid (5.00 g?L-1，7.50 g?L-1) was harmful to Solanum nigrum L.While the saponins concentrations were less than or equal 5.00 g?L-1 could promote Solanum nigrum L.growth and partly mitigate the toxicity of Cd to Solanum nigrum L.plants，the best effect was achieved with 5.00 g?L-1，but the result was not notable.However，higher concentrations of saponins (more than 7.50 g?L-1) could inhibit the growth of Solanum nigrum L.In general，the effect was better than the saponin of rhamnolipid.
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备注/Memo
第一作者简介：陈文志（1979-），男，硕士，讲师，研究方向为园林植物。E-mail：28089428@qq.com.责任作者：潘远智（1968-），男，教授，博士生导师，现主要从事园林植物培育与应用等研究工作。基金项目：国家自然科学基金资助项目（31670622）。