ZENG Bin,LIU Mengwen,WANG Jianyou,et al.Identification of S-genotypes in the Five Natural Populations and Analysis of S-RNase Genes Frequency From Xinjiang Wild Almond（Prunus tenella Batsch.)[J].Northern Horticulture,2017,41(10):87-92.[doi:10.11937/bfyy.201710020]
新疆野扁桃五个自然分布居群S基因型的鉴定及发生频率分析
- Title:
- Identification of S-genotypes in the Five Natural Populations and Analysis of S-RNase Genes Frequency From Xinjiang Wild Almond（Prunus tenella Batsch.)
- 文献标志码:
- A
- 摘要:
- 以新疆野扁桃5个自然分布居群的150个株系为试材，采用生物统计学的方法，研究了新疆野扁桃5个自然分布居群的S基因类型及其发生频率。旨在为新疆野扁桃资源的有效保护与合理利用及该物种自交不亲和性进化机理提供科学依据。结果表明：在受试株系中共鉴定出6个S-RNase基因，S基因型有11种类型，各基因型组成在不同居群中的存在比率是不均等的，有占主导地位的S基因型；还有一些S基因型是某个居群特有的；也有一些S基因型出现的比率很低，变化差异较大；表明S-RNase基因经历了一定的进化历程。
- Abstract:
- Taking 150 accessions of Prunus tenella Batsch.(wild Almond) as materials,using the statistics method,the Sgenotype group and its occurrence frequency were studied in the five populations of Prunus tenella Batsch.in Xinjiang China.The results showed that six S-RNase genes were identified in the tested accessions,eleven S-genotypes were determined,the S-genotypes were heterogeneous and some were dominant S-genotypes,and some were unique.There were some S-genotypes in the ratio was very low,the change was quite different,the aim of the study was to provide the scientific basis for the effective protection and reasonable utilization of the wild almond resources in Xinjiang and lay the foundation for the further study of the mechanism of self-incompatibility of Prunus tenella.
参考文献/References:
[1]de NETTANCOURT D.Incompatibility in angiosperms[J].Sex Plant Reprod,1977（10）:185-199. [2]CHEN G,ZHANG B,LIU L,et al.Identification of a ubiquitin-binding structure in the S-locus F-box protein controlling S-RNase-based self-incompatibility[J].Journal of Genetics and Genomics,2012,39:93-102. [3]LIU W,FAN J B,LIU J H,et al.SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrid[J].Plant Genetics and Genomics,2014（5）:1-15. [4]PESSINA S,PALMIERI L,BIANCO L,et al.Frequency of a natural truncated allele of 〖STBX〗〖JP〗MdMLO19 〖STBZ〗in the germplasm of Malus domestica[J].Mol Breeding,2017,37(1):7.doi:10.1007/s11032-016-0610-8. [5]陈慧,张树军,张妤艳,等.40个梨品种S基因型的鉴定及S基因频率分析[J].南京农业大学学报,2013,36(5):21-26. [6]QIAO X,LI M,LI L T,et al.Genome-wide identification and comparative analysis of the heat shock transcription factor family in Chinese white pear (Pyrus bretschneideri) and five other Rosaceae species[J].BMC Plant Biology,2015(15):12-25. [7]VAUGHAN S P,BOKOVI R I,GISBERT-CLIMENT A,et al.Characterisationofnovels-alleles from cherry (Prunus avium L.)[J].Tree Genet Genomes,2008(4):531-541. [8]CARRASCO B,MEISEL L,GEBAUER M,et al.Breeding in peach,cherry and plum：from a tissue culture,genetic,transcriptomic and genomic perspective[J].Biol Res,2013,46:219-230. [9]ORTEGA E,BOKOVI R I,SARGENI D J,et al.Analysis of S-RNase alleles of almond(Prunus duleis):Characterization of new sequences,resolution of synonyms and evidence of intragenic recombination[J].Mol Genet Genomies,2006,276:413-426. [10]QUAGLINO F,KUBE M,JAWHARI M,et al.‘Candidatus Phytoplasma phoenicium’ associated with almond witches-broom disease:From draft genome to genetic diversity among strain populations[J].BMC Microbiology,2015（15）:148-169. [11]张树军,黄绍西,张绍铃,等.槜李等15个李品种S基因型鉴定及其多态性分析[J].果树学报,2008,25(3):338-342. [12]WANG P P,GAO Z H,NI Z J,et al.Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume)[J].Genet Mol Res,2013,12(3):3286-3295. [13]刘崇琪,陈晓流,张艳敏,等.新疆野生樱桃李S-RNase基因分离与鉴定的初步研究[J].园艺学报,2009,36(3):333-340. [14]关鹏,曾斌,李疆,等.2个野扁桃自交不亲和S-RNase基因全长的克隆与序列分析[J].果树学报,2016,33(8):905-916. [15]SURBANOVSKI N,TOBUTT K R,KONSTANTINOVIC M,et al.Self-incompatibility of Prunus tenella and evidence that reproductively isolated species of Prunus have different SFB alleles coupled with an identical S-RNase allele[J].Plant J,2007,50:723-734. [16]HARRISON B D,LIU Y L,KHALID S,et al.Detection and relationship of cotton leaf curl virus and allied whitefly-transmitted geminiviruses occurring in Pakistan[J].Annals of Applied Biology,1997,130(1):61-75. [17]TAO R,YAMANE H,SUGIURA A.Molecular typing of S-alleles through identification,characterizationand cDNA cloning for S-RNases in sweet cherry[J].J Amer Soc Hort Sci,1999(24):224-233. [18]BATEMAN A J.Self-incompatibility in angiosperms.I.Theory[J].Heredity,1952(6):285-310. [19]BATEMAN A J.Self-incompatibility systems in angiosperms.III.Cruciferae[J].Heredity,1955(9):51-68. [20]MATTON D P,NASS N,CLARKE A E,et al.Self-incompatibility:How plants avoid illegitimate offspring[J].Proc Natl Acad Sci USA,1994,91:1992-1997. [21]RICHMAN A D,UYENOYAMA M K,KOHN J R.Allelic diversity and gene geneaology at the self-incompatibility locus in the Solanaceae[J].Science,1996,273:1212-1216. [22]UYENOYAMA M K.Genealogical structure among alleles regulating self-incompatibility in natural populations of flowering plants[J].Genetics,1997,147:1389-1400.
相似文献/References:
[1]刘楠楠,曾斌,李伟阳,等.异质生境的新疆野扁桃组培快繁体系比较研究[J].北方园艺,2015,39(17):85.[doi:10.11937/bfyy.201517023]
LIU Nannan,ZENG Bin,LI Weiyang,et al.Comparative Study on Establishment of Regenertation System of Tissue Rapid Propagation System of Heterogeneous Habitats Prunus tenella in Xinjiang[J].Northern Horticulture,2015,39(10):85.[doi:10.11937/bfyy.201517023]
[2]曾斌,刘梦雯,王建友,等.新疆蔷薇科扁桃类植物自交不亲和性的分子机制[J].北方园艺,2017,41(09):174.[doi:10.11937/bfyy.201709037]
ZENG Bin,LIU Mengwen,WANG Jianyou,et al.Molecular Mechanism Analysis of Genes Controlling Self-incompatibility in Xinjiang Rosaceae Amygdalus Plants[J].Northern Horticulture,2017,41(10):174.[doi:10.11937/bfyy.201709037]
[3]刘梦雯,曾斌,王波,等.新疆野扁桃TP-M13-SSR引物的多态性分析[J].北方园艺,2018,42(02):46.[doi:10.11937/bfyy.20171307]
LIU Mengwen,ZENG Bin,WANG Bo,et al.Polymorphism Analysis of TP-M13-SSR Primer for Prunus tenella Batsch.[J].Northern Horticulture,2018,42(10):46.[doi:10.11937/bfyy.20171307]
[4]杨红丽,胡靖锋,徐学忠,等.氯化钠硼砂溶液克服萝卜自交不亲和性的研究[J].北方园艺,2018,42(06):39.[doi:10.11937/bfyy.20173146]
YANG Hongli,HU Jingfeng,XU Xuezhong,et al.Study on Overcoming Radish Self-incompatibility by Sodium Chloride and Borax[J].Northern Horticulture,2018,42(10):39.[doi:10.11937/bfyy.20173146]
[5]殷亚蕊,武军凯,马聚泽,等.欧李自交不亲和S-RNase基因型鉴定及序列分析[J].北方园艺,2019,43(24):28.[doi:10.11937/bfyy.20191520]
YIN Yarui,WU Junkai,MA Juze,et al.Identification and Sequence Analysis of S-RNase Genes From Prunus humilis Bunge[J].Northern Horticulture,2019,43(10):28.[doi:10.11937/bfyy.20191520]
[6]曾斌,刘梦雯,王建友,等.新疆野扁桃六个新S-RNase基因的克隆和序列分析[J].北方园艺,2017,41(07):105.[doi:10.11937/bfyy.201707023]
ZENG Bin,LIU Mengwen,WANG Jianyou,et al.Molecular Identification Analysis of Six New S-RNase Genes Controlling Self-incompatibility From Xinjiang Wild Almond（Prunus tenella Batsch.）[J].Northern Horticulture,2017,41(10):105.[doi:10.11937/bfyy.201707023]
备注/Memo
第一作者简介：曾斌（1969-），男，湖北松滋人，博士，副教授，现主要从事果树种质资源及生物技术的教学与科研等工作。E-mail：zbxnd@163.com.基金项目：国家自然科学基金资助项目（31660557，31260465）；新疆维吾尔自治区果树学重点学科资助项目（201007）。