ZHENG Xiqing,DI Na,LI Xuhong,et al.Isolation and Screening of Phosphorus Solubilizing Bacterial Strains in Soil[J].Northern Horticulture,2017,41(09):164-168.[doi:10.11937/bfyy.201709035]
土壤中解磷细菌的分离筛选
- Title:
- Isolation and Screening of Phosphorus Solubilizing Bacterial Strains in Soil
- 文献标志码:
- A
- 摘要:
- 为筛选出高效的解磷微生物，提高磷的有效利用，采集向日葵、小麦、豆科等不同种植区单株的根际土壤，采用平板溶菌圈法从土壤中进行分离筛选，初步筛选出3株解磷菌JLX-1、JLX-2、JLX-3，研究不同条件对其解磷能力的影响。结果表明：菌株JLX-3的解磷能力较高，溶磷圈直径和菌落直径比值D/d为2.09。菌株JLX-3在碳源为葡萄糖、氮源为硫酸铵时，溶磷量分别为5.572、5.520 mg?g-1，在氯化钠浓度为0.3 g?L-1、pH 7时解磷效果较好。根据形态特征和生理生化特征，初步将菌株JLX-3鉴定为芽孢杆菌。
- Abstract:
- The experiment was conducted to study the phosphoru bacterial strains from the rhizosphere soil of the single sunflower,wheat and leguminosae planting areas by plate P-solubilizing circle method.Three phosphate-solubilizing bacterial strains including JLX-1，JLX-2 and JLX-3 were obtained from all kinds of corps soil to investigate the effects of different and JLX3 were abtained from all kinds of crops soil to investigate the effects of different conditions on Psolubilizing capacity.The results showed that the JLX-3 strains showed strong ability of dissolving phosphate with a ratio of dissolving phosphate zone diameter to culture community zone diameter (D/d) 2.09.When the carbon source was glucose,nitrogen source was (NH4)2SO4,the phosphate-solubilizing capacity were 5.572 mg?g-1 and 5.520 mg?g-1,respectively.The JLX-3 displayed a better phosphate solubilizing capability when NaCl concentration was 0.3 g?L-1 and pH was 7.The based on the morphological physiological and biochemical properties JLX-3 was identified as Bacillus.
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备注/Memo
第一作者简介：郑喜清（1979-），女，内蒙古兴安盟人，硕士，讲师，现主要从事植物病害综合防控技术等研究工作。E-mail:413703219@qq.com.基金项目：河套学院自然科学基金资助项目（HTXYZY13005）。