TAO Menghui,SUO Quanyi,ZHANG Shuguang,et al.Effects of Different Fertilization on the Number of Soil Microbiologic and Soil Enzyme Activities[J].Northern Horticulture,2017,41(09):154-159.[doi:10.11937/bfyy.201709033]
不同施肥对土壤微生物量和酶活性的影响
- Title:
- Effects of Different Fertilization on the Number of Soil Microbiologic and Soil Enzyme Activities
- Keywords:
- organic fertilizer; chemical fertilizer; microform; enzymatic activity
- 文献标志码:
- A
- 摘要:
- 通过无植物盆培养试验,研究施用化肥、有机肥、生物有机肥、化肥与有机肥混合施用、化肥与生物有机肥混合施用、对照处理对土壤中微生物数量和酶活性的影响。结果表明:施用生物有机肥土壤中细菌数量比施用化肥和有机肥处理分别高出15.5%~43.1%和3.5%~6.4%;放线菌数量分别高出58.2%~94.4%和9.8%~24.8%;真菌数量分别降低22.8%~59.5%和8.9%~19.3%。土壤中脲酶活性最高的是生物有机肥和化肥混合施用处理;生物有机肥处理土壤中碱性磷酸酶活性最高,高出有机肥处理1.6%~37.4%,高出化肥处理3.2%~38.9%;生物有机肥处理土壤中蔗糖酶活性最高,比有机肥高出50.9%~292.4%,比化肥高出67.6%~456.3%;各处理对过氧化氢酶的活性影响较小。施用生物有机肥可以更好地改善土壤微生物区系,增加土壤酶活性,有利于土壤肥力的提高。
- Abstract:
- The effect of using chemical fertilizer,organic fertilizer,biological organic fertilizer,organic fertilizer combined with chemical fertilizer,chemical fertilizer combined with biological organic fertilizer and no fertilizer treatment on the number of soil microbiologic and soil enzyme activities were investigated through an outdoor pots experiment.The results showed that using the biological organic fertilizer,the number of soil bacteria were 15.5%-43.1% and 3.5%-6.4% higher than chemical fertilizer and organic fertilizer treatments and the number of actinomycetes were 58.2%-94.4% and 9.8%-24.8% higher than chemical fertilizer and organic fertilizer treatments.But the numbers of fungus are 22.8%-59.5% and 8.9%-19.3% than chemical fertilizer and organic fertilizer treatments.Biological organic fertilizer combined with chemical fertilizer treatment had the highest urease activities.Biological organic fertilizer treatment had the highest number of phosphatase activities,1.6%-37.4% higher than organic fertilizer treatment and 3.2%-38.9% higher than chemical fertilizer treatment.Biological organic fertilizer treatment has the highest sucrose activities,50.9%-292.4% higher than organic fertilizer treatment and 67.6%-456.3% higher than chemical fertilizer treatment.Each treatment has small effect on the catalase activities.Using biological organic fertilizer can improve the soil microflora,raise the enzymatic activity and increase the soil fertility.
参考文献/References:
[1]赵秉强,梅旭荣.对我国土壤肥料若干重大问题的探讨[J].科技导报,2007(8):65-70. [2]翁添富,高建培,易锋,等.土壤重金属污染生物修复技术研究进展[J].科技导报,2009(4):93-97. [3]KLEMEDTSSON L,BERG P,CLARHOLM M.Microbial nitrogentransformation in the root environment of barley[J].Soil Biology and Biochemistry,1987,19:551-558. [4]SINGH J S,RAGHUBANSHI A S,SRIVASTAVA S C.Microbialbiomass act as a source of plant nutrients in dry tropicalforest and savanna[J].Nature,1989,338:499-500. [5]SRIVASTAVA S C,SINGH J S.Microbial C,N and P in drytropical forest soils:Effects of alternate land-uses andnutrient flux[J].Soil Biology and Biochemistry,1991,23(2):117-124. [6]陈琳,谷洁,高华,等.含铜有机肥对土壤酶活性和微生物群落代谢的影响[J].生态学报,2012,32(12):3912-3920. [7]邵丽,谷洁,张社奇,等.生物复混肥对土壤微生物功能多样性和土壤酶活性的影响[J].农业环境科学学报,2012,31(6):1153-1159. [8]ZHANG Q C,IMRAN H S,XU D T,et al.Chemical fertilizer and organic manure inputs in soil exhibit a vice versa pattern ofmicrobial community structure[J].Appl Soil Ecol,2012,57:1-8. [9]ZHAO Q Y,SHEN Q R,RAN W,et al.Inoculation of soil by Bacillus subtilis Y-IVI improves plant growth and colonizationof the rhizosphere and interior tissues of muskmelon(Cucumis melo L.)[J].Biol Fert Soils,2011(47):507-514. [10]LIU E K,LI X,SHEN Q R,et al.Enzyme activity in water-stable soil aggregates as affected by long-term application oforganic manure and chemical fertilizer[J].Pedosphere,2013,23(1):111-119. [11]张静,杨江舟,胡伟,等.生物有机肥对大豆红冠腐病及土壤酶活性的影响[J].农业环境科学学报,2012,31(3):548-554. [12]文启孝.土壤有机质研究法[M].北京:农业出版社,1984. [13]杨玉盛,仝川,高人,等.生态学实验与技术教程[M].福州:福建教育出版社,2008. [14]周礼恺.土壤酶学[M].北京:科学出版社,1987. [15]王冬梅,王春枝,韩晓日,等.长期施肥对棕壤主要酶活性的影响[J].土壤通报,2006,37(2):263-267. [16]刘芷宇.植物的磷素营养和土壤磷的生物有效性[J].土壤,1992,24(2):97-101. [17]樊军,郝明德.黄土高原旱地轮作与施肥长期定位试验研究:Ⅰ:长期轮作与施肥对土壤酶活性的影响[J].植物营养与肥料学报,2003,9(1):9-13. [18]FORMAN R T.Some general principles of landscape andregional ecology[J].Landsc Ecol,1995,10(3):133-142. [19]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:274-323. [20]袁英英,李敏清,胡伟,等.生物有机肥对番茄青枯病的防效及对土壤微生物的影响[J].农业环境科学学报,2011,30(7):1344-1350. [21]高雪莲,邓开英,张鹏,等.不同生物有机肥对甜瓜土传枯萎病防治效果及对根际土壤微生物区系的影响[J].南京农业大学学报,2012,35(6):55-60. [22]任爽,柳影,曹群,等.不同用量生物有机肥对苦瓜枯萎病防治及土壤微生物和酶活性的影响[J].中国蔬菜,2013(10):56-63. [23]DORAN J W,SARRANTONIO M,LIEBIG M A.Soil health and sustainability[J].Advance Agronomy,1996,56:2-54. [24]IBEKWE A M,KENNEDY A C,FROHNE P S,et al.Microbial diversity along a transect of agronomic zones[J].FEMS Microbiology Ecology,2002,39:183-191. [25]ALLISON V J,CONDRON L M,PELTZER D A,et al.Changes in enzyme activities and soil microbial communitycomposition along carbon and nutrient gradients at the Franz Josef chronosequence,New Zealand[J].Soil Bio Biochem,2007,39(7):1770-1781. [26]张鹏,贾志宽,路文涛,等.不同有机肥施用量对宁南旱区土壤养分、酶活性及作物生产力的影响[J].植物营养与肥料学报,2011,17(5):1122-1130. [27]丁雷,李俊华,赵思峰,等.生物有机肥和拮抗菌对土壤有效养分和土壤酶活性的影响[J].新疆农业科学,2011,48(3):504-510. [28]张炎华,吴敏,何鹏.土壤酶活性与土壤肥力关系的研究进展[J].安徽农业科学,2007,35(34):1113-1114.
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备注/Memo
第一作者简介:陶梦慧(1989-),女,内蒙古通辽人,硕士研究生,研究方向为土壤肥力与植物营养。E-mail:1198263495@qq.com.责任作者:索全义(1962-),男,内蒙古呼和浩特人,博士,教授,博士生导师,现主要从事土壤肥力与植物营养等研究工作。E-mail:paul98@sina.com.基金项目:内蒙古自治区自然科学基金资助项目(2015MS0309)。