SU Jiang,LU Jianming,CHEN Linfa,et al.Study on Growth and Antioxidative Physiology Characteristics Dynamics of Potted Dendrobium candidum[J].Northern Horticulture,2017,41(09):130-135.[doi:10.11937/bfyy.201709028]
盆栽铁皮石斛生长和抗氧化生理特性动态研究
- Title:
- Study on Growth and Antioxidative Physiology Characteristics Dynamics of Potted Dendrobium candidum
- Keywords:
- potting; Dendrobium candidum; growth; antioxidative physiology
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛组培苗为试材，以松树皮和桑杆为基质，采用盆栽方式种植铁皮石斛组培苗，研究盆栽对铁皮石斛组培苗生长的影响及其不同生长阶段抗氧化生理特性的变化。结果表明：2种不同基质盆栽铁皮石斛组培苗在不同生长阶段叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和脯氨酸含量均显著高于移栽前，丙二醛(MDA)含量稍高于移栽前，叶绿素含量较移栽前的低，且2种不同基质的铁皮石斛叶片POD、SOD、CAT活性差异明显，脯氨酸含量、MDA含量和叶绿素含量差异不明显；2种不同基质盆栽铁皮石斛组培苗成活率都达90%以上，且移栽后长势好，移栽后2年株高分别为56.18、61.42 cm，与大棚苗床栽培的效果比较接近。
- Abstract:
- Taking Dendrobium candidum as test material，the tissue culture seedling of Dendrobium candidum was potted in this experiment，and pine bark and mulberry rod were used as substrate.The effects of potting on growth and antioxidative physiology characteristics dynamics of Dendrobium candidum tissue culture seedling were sutudied in the cultivation process.The results showed that in the cultivation process，the activities of POD，SOD，CAT and the proline content of potted Dendrobium candidum tissue culture seedling with two different substrate was significantly higher than that before transplanting.Also the MDA content was slightly higher than that before transplanting，while the chlorophyll content was lower than that before transplanting.Moreover，the differences of the activities of POD，SOD，CAT were obvious between the two substrates.While the difference of the proline content，the MDA content，the chlorophyll content were not obvious.The survival rate of the Dendrobium Candidum tissue culture seedling was more than 90% of both the substrates，meanwhile，the growth was well.The plant height of the Dendrobium candidum tissue culture seedling was 56.18 cm and 61.42 cm respectively after transplantation two years of the two substrates which was closed to the effect of greenhouse seedbed cultivation.
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备注/Memo
第一作者简介：苏江(1980-)，女，壮族，硕士，讲师，现主要从事植物组织培养及植物生理生化等研究工作。E-mail:zhongyong20@163.com.基金项目：广西教育厅资助项目（2013YB207）;广西高校微生物及植物资源开发利用重点实验室（河池学院）资助项目(桂教科研（2014）6号);河池学院校级重点实验室资助项目(校政发(2016)91号)。