LI Qiaozhen,SHANG Xiaodong,SONG Chunyan,et al.Free Amino Acids Content Change of Pleurotus eryngii During Cultivation[J].Northern Horticulture,2017,41(09):125-129.[doi:10.11937/bfyy.201709027]
刺芹侧耳栽培过程中游离氨基酸含量的变化
- Title:
- Free Amino Acids Content Change of Pleurotus eryngii During Cultivation
- 文献标志码:
- A
- 摘要:
- 以刺芹侧耳不同生长发育期的基质菌丝体和子实体为试材，采用氨基酸自动分析仪对刺芹侧耳栽培过程中基质菌丝体和子实体的游离氨基酸含量的动态变化进行了研究，以期掌握刺芹侧耳整个栽培周期中基质菌丝体和子实体中氨基酸含量的变化动态，为进一步探索刺芹侧耳栽培过程中菌丝吸收利用氮后合成的有机氮载体以及转运和传递方式奠定基础。结果表明：基质菌丝体中总游离氨基酸含量在发菌阶段显著上升，在后熟阶段及搔菌处理时期，总游离氨基酸含量变化不显著；子实体生长阶段，基质菌丝体中总游离氨基酸含量逐渐下降。基质菌丝体中含量最丰富的氨基酸为精氨酸（Arg），现原基时含量达到最高值为7 082.30 μg?g-1（占比65.86%），从现原基到子实体成熟过程中基质菌丝体中Arg含量逐渐下降，最终为1 741.31 μg?g-1（占比40.67%）。由此推测Arg在刺芹侧耳生长过程中的氮素营养转运中起到重要作用。
- Abstract:
- In order to grasp the change regularity of amino acids content in the substrate mycelium and in the fruit body of Pleurotus eryngii during the growth，and lay the foundation for exploring further organic nitrogen carrier and nitrogen transfer method after hypha absorb nitrogen，the amino acids contents were determined in the substrate mycelium and in the fruit body of Pleurotus eryngii at different development stages with automatic analyzer amino acids.The results showed that the content of total free amino acids in the substrate mycelium increased significantly at the stage of spawn running，and decreased gradually at the fruiting stage.The most abundant amino acids in the substrate mycelium was arginine，the content of arginine reached the highest value 7 082.30 μg?g-1 （65.86%） at primordium phase，and gradually declined from the primordium phase to the fruiting body matured stage，which finally was 1 741.31 μg?g-1（40.67%）.This suggested the arginine played an important role in nitrogen transport during the growth of P.eryngii.
参考文献/References:
[1]郭美英.珍稀食用菌杏鲍菇生物学特性的研究[J].福建农业学报,1998,13(3):44-49. [2]王家季,刘化民.培养料碳氮比对金针菇生长发育及产量的影响[J].中国食用菌,2004(4):30-31. [3]贾素巧.碳氮营养对猴头菌生长发育及胞外酶活性的影响[D].保定:河北农业大学,2006. [4]李伟平.碳氮营养对秀珍菇生长发育及胞外酶活性的影响[D].保定:河北农业大学,2007. [5]JIN H R，PFEFFER P E，DOUDS D D，et al.The uptake，metabolism，transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis[J].New Phytol,2005,168:301-310. [6]CRUZ C,EGSGAARD H,TRUJILLO C,et al.Enzymatic evidence for the key role of arginine in nitrogen translocation by arbuscular mycorrhizal fungi[J].Plant Physiology,2007,144:782-792. [7]JIN H R.Arginine bi-directional translocation and breakdown into ornithine along the arbuscular mycorrhizal mycelium[J].Sci China Ser C-Life Sci,2009,52:381-389. [8]BORSUK P,DZIKOWSKA A,EMPEL J,et al.Structure of the arginase coding gene and its transcript in Aspergillus nidulans[J].Acta Biochim Pol,1999,46:391-403. [9]DZIKOWSKA A,KACPRZAK M,TOMECKI R,et al.Specific induction and carbon/nitrogen repression of arginine catabolism gene of Aspergillus nidulans functional in vivo analysis of the otaA promoter[J].Fung Genet Biol,2003,38:175-186. [10]WAGEMAKER M J M,EASTWOOD D C,van der DRIFT C,et al.Argininosuccinate synthetase and argininosuccinate lyase:Two ornithine cycle enzymes from Agaricus bisporus[J].Mycol Res,2007,111:493-502. [11]张引芳,刘遐,陈建华,等.杏鲍菇工厂化生产工艺研究[J].食用菌学报,2003,10(2):36-39. [12]于海龙.可控环境下杏鲍菇子实体发育模型研究[D].南京:南京农业大学,2009:70-81.
相似文献/References:
[1]李 超,肖千明.刺芹侧耳液体培养基配方优化研究[J].北方园艺,2014,38(09):168.
LI Chao,XIAO Qian-ming.Study on Culture Medium Formulation of Pleurotus eryugii (DC.:Fr.) Quel Liquid Spawn[J].Northern Horticulture,2014,38(09):168.
备注/Memo
第一作者简介：李巧珍（1986-），女，硕士，助理研究员，研究方向为食用菌栽培育种。E-mail：liqiaozhen-345@163.com.责任作者：鲍大鹏（1969-），男，博士，研究员，研究方向为食用菌遗传。E-mail：1610301025@qq.com.基金项目：上海市科技兴农重点攻关资助项目（沪农科攻字 2015 第6-2-1）。