LI Yanqing,ZHAO Yucui,PAN Haoqin.Pesticide Effect Experiment and Identification of Phomopsis vexans From Eggplant in Shouguang of Shandong Province[J].Northern Horticulture,2017,41(09):106-110.[doi:10.11937/bfyy.201709023]
山东寿光茄子褐纹病病原鉴定及药效试验
- Title:
- Pesticide Effect Experiment and Identification of Phomopsis vexans From Eggplant in Shouguang of Shandong Province
- Keywords:
- eggplant Phomopsis rot; isolation and identification; pathogenicity; antibacterial experiment
- 文献标志码:
- A
- 摘要:
- 以携带褐纹病菌的茄子叶片为试材，采用室内分离鉴定、致病性测定、室内抑菌试验等方法进行病原分离，并研究了病原菌的致病性及抗药性，为田间茄子褐纹病的诊断与防治提供参考依据。结果表明：共分离到9个菌株，菌株类型有产生α型分生孢子、β型分生孢子、混合型分生孢子及只产生菌丝不产生载孢体类型；致病性测定发现菌株PvTF-11、PvVSJ-22、PvVL-211对茄子叶片致病性较强；茄子褐纹病菌的室内抑菌试验发现PvVL-621、PvVL-211属于药剂敏感型菌株，PvVSJ-101、PvVSJ-102属于抗药型菌株。说明在人工培养条件下茄子褐纹病菌也可以产生β型分生孢子及混合型分生孢子类型；且茄子褐纹病菌已产生抗药性菌株。
- Abstract:
- Leaves of eggplant with Phomopsis rot bacteria were used as materials.Via indoor separation identification，determination of pathogenic and bacteriostatic test methods，the pathogenic bacteria were isolated and the pathogenicity and resistance to drugs were studied.The results showed that nine strains were obtained，which containing generating α-conidia，β-conidia，hybrid conidia and only making mycelium type.PvTF-11，PvVSJ-22 and PvVL-211 had stronger pathogenicity for eggplant leaf by determination of pathogenic.Antibacterial experiment found that PvVL-621，PvVL-211 belonged to the drug sensitive strains，PvVSJ-101，PvVSJ-102 type belonged to the resistant strains.So eggplant Phomopsis rot bacteria could also produce β-conidia and hybrid conidiophore type under the condition of artificial culture.And eggplant Phomopsis rot bacteria had resistant strains.
参考文献/References:
[1]HARTER.Fruit-rot，leaf-spot，and stem-blight of the eggplant caused by Phomopsis vexans[J].J Agricul Res，1914（5）:331-338. [2]刘学敏,任锡伦,李润霞,等.茄子对褐纹病(Phomopsis vexans)的抗性遗传研究[J].吉林农业大学学报,1998,20(4):1-7. [3]周永力,吕国忠,刘伟成,等.球壳抱目真菌个体发育研I:壳二胞等四属[J].菌物系统,1998，17(3)：199-205. [4]吴仁锋,杨绍丽,杨德枝.茄子褐纹病病原鉴定及其生物学特性研究[J].中国蔬菜,2013(8)：80-85. [5]陈姗姗,宋述尧,韩玉珠,等.不同抗性茄子品种保护酶系统对褐纹病菌的抗性应答[J].西北农林科技大学学报(自然科学版),2015,43(11):172-180. [6]杨绍丽,吴仁锋,马晓龙,等.湖北省茄子品种抗褐纹病鉴定[J].中国蔬菜,2016(1):64-66. [7]陈姗姗,宋述尧,赵春波,等.茄子褐纹病抗性与果皮超微结构的关系[J].西北农林科技大学学报(自然科学版),2016,44(9):143-150. [8]罗丽娟,习平根,姜子德,等.纯培养拟茎点霉属真菌的产孢条件[J].菌物学报,2004,23(2)：219-225. [9]魏景超.真菌鉴定手册[M].上海:上海科学出版社,1979． [10]戚佩坤,姜子德,向梅梅.中国真菌志[M].北京:科学出版社,2007. [11]罗丽娟,习平根,姜子德,等.纯培养下拟茎点霉分生孢子的形成和意义[J].菌物学报,2004，23(3)：375-380.
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备注/Memo
第一作者简介：李艳青（1983-），女，博士，副教授，现主要从事蔬菜病理学等研究工作。E-mail：lyq680600@163.com.基金项目：潍坊市科学技术发展计划资助项目（2014GX048）。