SUN Honghuan,WANG Yong,LI Lei,et al.Separation of Allelochemicals of the Sixth Chromatography Fractions of Ethylalcohol Extracts of Fresh Parsley Roots and Its Allelopathic Effects on Fusarium oxysporum f.sp.cucumberinum[J].Northern Horticulture,2017,41(09):93-100.[doi:10.11937/bfyy.201709020]
西芹鲜根六次醇层物化感物质的分离及其对黄瓜枯萎病菌的化感作用
- Title:
- Separation of Allelochemicals of the Sixth Chromatography Fractions of Ethylalcohol Extracts of Fresh Parsley Roots and Its Allelopathic Effects on Fusarium oxysporum f.sp.cucumberinum
- Keywords:
- parsley fresh root; the sixth chromatography fractions of ethylalcohol extracts; chromatography and separation; Fusarium oxyporum f.sp.cucumerinum; allelopathy; allelochemicals
- 文献标志码:
- A
- 摘要:
- 以西芹鲜根为试材，经乙醇浸提后，采用注层析法、化感作用检测法和GC-MS检测法，研究了西芹鲜根乙醇浸提液经第5、6次层析分离获得各层析流分与黄瓜枯萎病菌共培养，并计算出化感效果筛选出最佳流分，最后通过GC-MS检测出6次醇层物最佳流分中的化感物质成分，从而达到西芹鲜根中化感物质成分的纯化目的。结果表明：各流分均对黄瓜枯萎病菌具有较强抑制作用，其化感效果与乙醇对照差异显著，第5次层析最佳流分为RE211018、RE29554、RE62359和RE68656，化感效果（相对于乙醇对照）依次为63.05%、70.84%、69.38%和59.16%；第6次层析最佳流分为RE2110186、RE295548、RE623597和RE686568，其化感效果（同上）分别提高至70.14%、71.38%、64.84%和60.63%。西芹鲜根第6次醇层物4个最佳流分中含有酚类、酯类、胺类和烷烃类共4类11种化感物质，分别为2,4-二叔丁基苯酚、8,11-十八碳二烯酸甲酯、芥酸乙酯、邻苯二甲酸二甲酯、邻苯二甲酸异丁酯、邻苯二甲酸丁基2-乙基己基酯、邻苯二甲酸丁基酯2-戊基酯、邻苯二甲酸二丁酯、(Z)-9-十八烯酸酰胺、二十七烷和3-乙基-5-(2-乙基丁基)十八烷。
- Abstract:
- Athylalcohol extracts of fresh parsley roots was used as the experimental material,and the methods has been applied with chromatography by a column with silicone coating,allelopaphy testing and GC-MS.We studied that ethylalcohol extracts of fresh parsley roots has been proceed with the fifth and sixth chromatography fractions and incorporated the different degree column fractions into PDA medium and co-cultured the plates with F.oxysporum f.sp. cucumberinum.The best fractions of ethylalcohol extracts of parsley fresh root were screened according to the allelopathic inhibition effect.At last，further allelochemicals of the sixth chromatography was identified by GC-MS.So as to achieve the purpose of identifing inhibitory allelochemicals released into the soil by parsley crop.The results showed that there were significant different allelopathic effect for the degree column fractions obtained from the fifth and sixth cycles of chromatography respectively on F.oxysporum f.sp. cucumberinum.The allelopathic effects of the fifth optimal fractions (RE211018,RE29554,RE62359 and RE68656) compared with the fifth cycle tomographic ethylalcohol (the control，ECK1) was 63.05%,70.84%,69.38% and 59.16% respectively.The allelopathic effects of the sixth optimal fractions (RE2110186,RE295548,RE623597 and RE686568) compared with the sixth cycle tomographic ethylalcohol (the control，ECK2) was 70.14%,71.38%,64.84% and 60.63% respectively.There were 11 kinds of allelochemicals contained in Phenol，ester，amine and alkanes 4 classes in the GC-MS analysis of the sixth chromatography fractions of ethylalcohol extracts of parsley fresh root.They were 2,4-bis (1,1-dimethylethyl)-phenol，8,11-octadecadienoic acid,methyl ester，Ethyl 13-docosenoate (ethyl erucate)，Dimethyl phthalate,1,2-benzenedicarboxylic acid，butyl 2-methylpropyl ester，1,2-benzenedicarboxylic acid,butyl 2-ethylhexyl ester,phthalic acid，butyl 2-pentyl ester,dibutyl phthalate,9-Octadecenamide,(Z)-,heptacosane，3-ethyl-5-(2-ethylbutyl)-octadecane.
参考文献/References:
[1]HOU Y X，HU X J，ZHOU B L.Hot pepper growth promotion and inhibition of Fusarium wilt (Fusarium oxysporum) with different crop stalks[J].African Journal of Agricultural Research，2012，7(35)：5005-5011. [2]RICE E L.Allelopathy[M].2nd ed.New york：Academic Press.1974. [3]RICE E L.Allelopathy(2rk edition)[M].New York:Academic Press,1984:1-50. [4]W I T TACKERR H，FEENY P P.Allelochemics：Chemical interactions between species[J].Science，1971,171(3937)：757-770. [5]刘成.芦苇化感作用及其化感物质分离与鉴定[D].重庆：西南大学,2014. [6]陈磊,云兴福.西芹鲜根及根际区物化感物质成分鉴定[J].华北农学报,2012(2):157-164. [7]NYCHAS G J E.Natural antimicrobials from plants[J].New Methods Food Preserve，1995(1):58-89. [8]袁高庆,黎起秦,叶云峰,等.植物化感作用在植物病害控制中的应用[J].广西农业科学,2009,40(8):1017-1020. [9]张东东.西芹浸提液对黄瓜枯萎病菌化感作用机理的研究[D].呼和浩特：内蒙古农业大学,2013. [10]贾俊英,云兴福.西芹种子浸提液对黄瓜枯萎病菌的化感作用机理研究[J].北方园艺,2013(20):105-108. [11]GIL S V，MERILES J，CONFORTO C，et al.Response of soil microbial communities to different management practices in surface soils of a soybean agroecosystem in Argentina[J].European Journal of Soil Biology, 2011，47(1)：55-60. [12]SENARATHNE H S，DISSANAYAKA N M，VIDHANA A L P.Allelopathic potential of Brachiaria brizantha and B.milliformis on seed germination of selected bioassay species[J].Pakistan Journal of Weed Science Research，2010,16(2)：207-216. [13]陈磊.西芹根物质与挥发物对黄瓜枯萎病菌的化感作用、机理及化感物质分离纯化的研究[D].呼和浩特:内蒙古农业大学,2012. [14]郝静,高晓敏,马立国,等.西芹根物质四次醇层物对黄瓜枯萎病菌的化感作用[J].内蒙古农业大学学报(自然科学版),2015(3):26-32. [15]高晓敏.西芹浸提液化感物质分离鉴定及其对黄瓜枯萎病诱导抗性的研究[D].呼和浩特:内蒙古农业大学,2015. [16]高晓敏,王琚钢,李杰,等.西芹鲜根丙酮浸提物层析流分对黄瓜枯萎病菌的化感作用以及化感物质鉴定[J].中国生态农业学报,2014,22(11):1364-1371. [17]周文丽.西瓜根系分泌物与枯萎病菌之间的相互作用研究[D].保定：河北农业大学,2014. [18]杨阳.分蘖洋葱根系分泌物对黄瓜的化感作用及其应用[D].哈尔滨：东北农业大学,2010. [19]甄文超,王晓燕,曹克强,等.草莓根系分泌物和腐解物中氨基酸的检测及其化感作用研究[J].河北农业大学学报,2004,27(2):76-80. [20]徐宁,王超,魏珉，等.大葱根系分泌物对黄瓜种子萌芽和枯萎病病原菌的化感作用及其GC-MS分析[J].园艺学报,2012,39(8):1511-1520. [21]刘娜,周宝利,李轶修,等.茄子/番茄嫁接植株根系分泌物对茄子黄萎病菌的化感作用[J].园艺学报,2008,35(9):1297-1304. [22]王小兵,骆永明,刘五星,等.花生根分泌物的鉴定及其化感作用[J].生态学杂志,2011,30(12):2803-2808. [23]郝文雅.西瓜、水稻根系分泌物中的化感物质对西瓜连作枯萎病的影响[D].南京:南京农业大学,2010. [24]廖薇,张健,杨婉身,等.青花椒根际土壤化感物质成分分析[J].四川农业大学学报,2006(1):47-50. [25]韩丽梅,王树起,鞠会艳,等.吸附树脂提取的大豆根分泌物种类的GC-MS分析[J].大豆科学,2003(4):301-305. [26]娄宁,李亚,李瑜.白毛银露梅挥发性化学成分研究[J].兰州大学学报,2004,40(4):58-60. [27]佟飞.大蒜植株水浸液的化感作用和抑菌作用[D].杨凌:西北农林科技大学,2007. [28]谢星光，陈晏，卜元卿，等.酚酸类物质的化感作用研究进展[J].生态学报,2014,53(77)：6417-6428. [29]操海群,岳永德.毛竹提取物的抑菌活性及其有效成分的初步分离[J].植物病理学报,2005,35(5)：428-433. [30]刘艳霞,李想,蔡刘体,等.烟草根系分泌物酚酸类物质的鉴定及其对根际微生物的影响[J].植物营养与肥料学报，2016,22(2):418-428. [31]崔翠,蔡靖,张硕新.核桃根系分泌物化感物质的分离与鉴定[J].林业科学,2013(2):54-60. [32]饶洪冲,段琰,朱征宇.苯酚消毒剂中邻苯基本酚和对叔戊基苯酚的高效液相色谱测定法[J].医药病理学报,2013,8(2):46-47. [33]董晓宁,高承芳,张骁佩,等.多花黑麦草根系抑草潜力评价及其化感物质分析[J].草业学报,2013,22(4):61-68.
相似文献/References:
[1]苏荣,郝娟,秦立金,等.西芹鲜根丙酮浸提液化感物质分离及其对黄瓜枯萎病菌的化感作用[J].北方园艺,2016,40(21):120.[doi:10.11937/bfyy.201621030]
SU Rong,HAO Juan,QIN Lijin,et al.Allelochemicals Isolation of Acetone Extracts From Fresh Parsley Root and Allelopathic Effect on Fusarium oxysporum f.sp. cucumberinum[J].Northern Horticulture,2016,40(09):120.[doi:10.11937/bfyy.201621030]
备注/Memo
第一作者简介：孙洪欢（1991-），女，硕士研究生，研究方向为高寒地区蔬菜栽培与生理。E-mail:962046507@qq.com.责任作者：云兴福（1958-），男，硕士，教授，博士生导师，现主要从事高寒地区蔬菜栽培与生理等研究工作。E-mail:yxf5807@163.com.基金项目：国家农业科技成果转化资金资助项目（2014GB2A400716)；内蒙古自治区应用技术研究与开发资金资助项目（20150136）。