[1]李阳,于锡宏,蒋欣梅,等.不同温度层积处理对北五味子种子休眠过程中种胚后熟的影响[J].北方园艺,2017,41(20):140-144.[doi:10.11937/bfyy.20170766]
　LI Yang,YU Xihong,JIANG Xinmei,et al.Effects of Different Temperature Stratification Treatments on Seed Germination of Schisandra chinensis Seed During Dormancy[J].Northern Horticulture,2017,41(20):140-144.[doi:10.11937/bfyy.20170766]
点击复制

不同温度层积处理对北五味子种子休眠过程中种胚后熟的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 140-144 栏目: 出版日期: 2017-10-30
Title:
Effects of Different Temperature Stratification Treatments on Seed Germination of Schisandra chinensis Seed During Dormancy
作者:
李阳1; 于锡宏1; 2; 蒋欣梅1; 2; 王建2
1.东北农业大学 农业部东北地区园艺作物生物学与种质创制重点实验室，黑龙江 哈尔滨 150030;2.黑龙江省林下经济资源研发与利用协同创新中心，黑龙江 哈尔滨 150040
Author(s):
LI Yang1; YU Xihong1; 2; JIANG Xinmei1; 2; WANG Jian2
1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Northeast Region，Ministry of Agriculture，Northeast Agriculture University,Harbin,Heilongjiang 150030；2.Heilongjiang Collaborative Innovation Center for Development and Utilization of Forest Resourcest，Harbin,Heilongjiang 150040
关键词:
北五味子; 变温层积; 呼吸底物; 激素
Keywords:
Schisandra chinensis; temperature change stratification; respiration substrate; hormone
DOI:
10.11937/bfyy.20170766
文献标志码:
A
摘要:
以北五味子种子为试材，采用不同温度层积处理的方法,研究了变温层积处理对北五味子种子休眠过程中种胚后熟的影响。结果表明：持续一定时间(90 d）的12 ℃高温层积处理(T1）和12 ℃高温层积后结合-4 ℃低温变温层积处理(T3、T4、T5，处理天数均为90 d）均有利于北五味子种胚后熟，使种胚占有率显著增加，而变温层积处理比持续高温处理T1更有利种胚后熟，其中12 ℃层积60 d后再进行-4 ℃低温层积30 d的处理(T5）最佳，其种胚占有率最大，种胚的成熟度最高。随着层积处理天数的增加，种子内的可溶性糖、可溶性蛋白质、脱落酸含量均下降，赤霉素含量表现为先下降后上升趋势，说明种胚的后熟需要消耗更多的可溶性糖、可溶性蛋白质、脱落酸、赤霉素含量的升高有利于解除休眠促进种胚后熟。
Abstract:
The seeds of Schisandra chinensis were used as test materials，using the method of different temperature stratification，the effects of temperature stratification in the process of embryo after ripening of seed dormancy of Schisandra chinensis.were studied.The results showed that 12 ℃ stratification for 90 days (T1) and 12 ℃ stratification combined with -4 ℃stratification (T3，T4，T5 treatment，treatment days were 90 days) were benefit for embryo development of Schisandra chinensis seed，the ratio of embryo sharing were raised after treatment.While the variable temperature stratification was more beneficial for embryo development than 12 ℃ stratification for 90 days.The development of seed embryo was the best after treated by 12 ℃ stratification for 60 days combined with -4 ℃ stratification for 30 days(T5) compared with other treatments.In the progress of breaking dormancy，the content of soluble sugar，soluble protein and abscisic acid were decreased over time，while，the content of gibberellin decreased after an initial increase，which indicated that more soluble sugar and soluble protein be consumed and endogenous hormone such as abscisic acid，gibberellin could speed up embryo maturation and break dormancy of Schisandra chinensis seed.

参考文献/References:

[1]汪兴汉.野生蔬菜的开发与利用[M].北京:中国农业出版社,2002:1-3.

[2]陈瑛,孙昌高,孙国栋,等.药用植物种子休眠的类型及特点[J].药学学报,1984,19(1):69-71.
[3]李合生.植物生理生化实验原理和技术[M].北京:高等教育出社，2000.
[4]章晓波,倪安丽,张文明,等.药用植物种子休眠的研究进展[J].中草药,1997(6):376-378.
[5]刘雅帅.山茱英种子休眠机理研究[D].南京:南京林业大学,2008.
[6]涂淑萍,穆鼎,刘春,等.百合鳞茎低温解除休眠过程中的生理生化变化研究[J].江西农业大学学报,2005,27(3):404-407.
[7]颜启传.种子学[M].北京:中国农业出版社,2001:169-170.
[8]张新娥.茄子种子处理对种子活力及幼苗生长的影响和机理研究[D].保定:河北农业大学,2010.
[9]BEWLEY J D.Seed Germination and Dormancy[J].The Plant Cell,1997,9(7):1055-1066.
[10]GALLARDO K,JOB C,GROOT S P,et al.Proteomics of Arabidopsis seed germination.A comparative study of wild-type and gibberellin-deficient seeds[J].Plant Physiology,2002,129(2):823-837.

相似文献/References:

[1]马凤霞,赵 权,刘广娜.乙烯利和脱落酸对北五味子木脂素及苯丙氨酸解氨酶活性的影响[J].北方园艺,2014,38(13):155.
　MA Feng-xia,ZHAO Quan,LIU Guang-na.Effect of Ethephon and Abscisic Acid on the Lignans and PAL Enzyme Activity of Schisandra chinensis（Turcz）Baill[J].Northern Horticulture,2014,38(20):155.
[2]张正海,张悦,赵伟伟,等.北五味子种质资源调查与分析[J].北方园艺,2013,37(13):185.
　ZHANG Zheng-hai,ZHANG Yue,ZHAO Wei-wei,et al.Investigation and Analysis of Germplasm Resources of ?Schisandra chinensis (Turcz.) Baill.[J].Northern Horticulture,2013,37(20):185.
[3]李玉环,李爱民,张正海,等.影响北五味子有效成分含量的因素研究[J].北方园艺,2013,37(14):164.
　LI Yu-huan,LI Ai-min,ZHANG Zheng-hai,et al.Study on Factors Affecting the Content of Active Ingredient of Schisandra[J].Northern Horticulture,2013,37(20):164.
[4]于海燕.生根粉对北五味子幼苗的影响[J].北方园艺,2012,36(03):166.
　YU Hai-yan.Effect of ABT on Seedlings of Schisandra chinensis (Turcz.) Baill.[J].Northern Horticulture,2012,36(20):166.
[5]程振玉,宋海燕,杨英杰,等.AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究[J].北方园艺,2015,39(07):141.[doi:10.11937/bfyy.201507041]
　CHENG Zhen-yu,SONG Hai-yan,YANG Ying-jie,et al.Study on Technology of Static Purification of Lignans from Schisandra chinensis Baill by Macroporous Adsorption Resin[J].Northern Horticulture,2015,39(20):141.[doi:10.11937/bfyy.201507041]
[6]王 艳 玲,奚 广 生.北五味子种子诱导分化培养基筛选研究[J].北方园艺,2009,33(07):0.[doi:10.11937/bfyy.200907090]
　W ANG Yan - ling,XI Guang- sheng.Screening Culture Medium of Schisandra sphenanthera Rehd. et Wils’ s Induction and Differentiation.[J].Northern Horticulture,2009,33(20):0.[doi:10.11937/bfyy.200907090]
[7]邓 　 勋,宋 小 双,周 　 琦,等.无公害农药防治北五味子白粉病田间药效试验[J].北方园艺,2008,32(02):0.[doi:10.11937/bfyy.2008020101]
　DENG Xun,SONG Xiao -shuang,ZHOU Qi,et al.Prevention Experiment of Non-public Hazard Medicine to Schisandra chinensis Powdery Mildew[J].Northern Horticulture,2008,32(20):0.[doi:10.11937/bfyy.2008020101]
[8]刘 　 军.五 味 子 播 种 扦 播 试 验[J].北方园艺,2007,31(06):0.[doi:10.11937/bfyy.200706057]
　[J].Northern Horticulture,2007,31(20):0.[doi:10.11937/bfyy.200706057]
[9]刘秋月,张萌,彭国红,等.北五味子应对逆境胁迫的生理机制研究进展[J].北方园艺,2022,(12):124.[doi:10.11937/bfyy.20214249]
　LIU Qiuyue,ZHANG Meng,PENG Guohong,et al.Physiological Mechanism of Schisandra chinensis Under Stress[J].Northern Horticulture,2022,(20):124.[doi:10.11937/bfyy.20214249]

备注/Memo

第一作者简介：李阳(1991-），男，黑龙江哈尔滨人，硕士研究生，研究方向为树芽类山野菜快速繁殖。E-mail：26668039@qq.com.

责任作者：蒋欣梅(1968-），女，浙江诸暨人，硕士，副教授，硕士生导师，现主要从事蔬菜栽培与生理等研究工作。E-mail:jxm0917@163.com.
基金项目：国家重点研发计划子课题资助项目(2016YFC0500307-06）。
收稿日期：2017-03-27

更新日期/Last Update: 2017-11-01