ZHANG Wanping,LI Xiaohui,MA Chao,et al.Research Progress on Mechanism of Bolting and Selection and Breeding of Anti-bolting Germplasms in Raphanus sativus L[J].Northern Horticulture,2017,41(07):192-199.[doi:10.11937/bfyy.201707042]
萝卜抽薹机制及耐抽薹种质资源选育的研究进展
- Title:
- Research Progress on Mechanism of Bolting and Selection and Breeding of Anti-bolting Germplasms in Raphanus sativus L
- Keywords:
- Raphanus sativus L.; anti-bolting; genetic analysis; CMS; molecular marker
- 文献标志码:
- A
- 摘要:
- 萝卜是我国广泛栽培的重要蔬菜作物，但先期抽薹成为严重影响萝卜品质和产量的重要因素，选育耐抽薹萝卜品种是解决该问题的重要手段。该研究通过分析近年来在萝卜耐抽薹性的遗传、生理生化和分子机制，以及萝卜耐抽薹种质资源的鉴定和培育等方面的研究进展，旨在更深入了解萝卜耐抽薹的机制、种质资源遗传多样性及雄性不育性等方面的理解和认识，从而为耐抽薹萝卜新种质创新提供理论指导。
- Abstract:
- Raphanus sativus L. is one of the most important vegetative crops cultivated widely in China.However，pre-mature bolting has become one of the limiting factors for the yield and quality of Raphanus sativus L..The effective approach to solve this problem is to breeding the anti-bolting lines.This study reviewed the recent progress on the genetic，physiological，biochemical and molecular mechanisms of anti-bolting in radish，as well as that about the screening and identification of Raphanus sativus L. germplasms related to anti-bolting.It would help us to gain deeper understanding about the anti-bolting mechanisms and the genetic diversity，as well as the CMS in Raphanus sativus L.，thus benefiting the breeding program for anti-bolting varieties in Raphanus sativus L. in China.
参考文献/References:
[1]NISHIJIMA T,KATSURA N,KOSHIOKA M,et al.Role of endogenous gibberellins in cold-induced stem elongation and flowering of Japanese radish (Raphanus sativus L.)[J].J Japan Soc Hortie Sci,1998,67:319-324. [2]张美霞,杨延杰,林多.抽薹进程中萝卜肉质根主要质地指标变化的研究[J].北方园艺,2013(24)：6-10. [3]张素君,邱杨,宋江萍,等.萝卜种质资源耐抽薹性鉴定评价[J].植物遗传资源学报,2014,15(2):262-269. [4]杨金兰,刘艳波,张舜.萝卜生产中抽薹的原因及其预防措施[J].北方园艺,2015(23):71-73. [5]王阳.11个萝卜自交系的杂种优势及配合力分析[D].武汉:华中农业大学,2014. [6]汪精磊,李锡香,邱杨,等.十字花科蔬菜抽薹开花性状的调控机理和分子育种研究进展[J].植物遗传资源学报,2015,16(6):1283-1289. [7]程斐,李式军.大白菜抽蔓性状的遗传规律研究[J].南京农业大学学报,1999,22(1):26-28. [8]余阳俊,张凤兰,赵帕云,等.大白菜及种内杂种小白菜×大白菜、芜著×大白菜的晚抽蔓遗传效应研究[J].华北农学报,2005,20(3):17-21. [9]SCHRANZ M E,QUIJADA P,SUNG S B,et al．Characterization and effects of the replicated flowering time gene FLC in Brassica rapa[J].Genetics,2002,162(3):1457-1468. [10]ZHAO J,KULKARNI V,LIU N,et al.〖STBX〗BrFLC2 〖STBZ〗( FLOWEＲING LOCUS C) as a candidate gene for a vernalization response QTL in Brassica rapa[J].J Exp Bot,2010,61(6):1817-1825. [11]KITAMOTO N,YUI S,NISHIKAWA K,et al.A naturally occurring long insertion in the first intron in the Brassica rapa 〖STBX〗FLC2 〖STBZ〗gene causes delayed bolting[J].Euphytica,2014,196(2):213-223. [12]纪小红,尹乐,沈宝宇,等.白菜抽薹相关性状遗传分析[J].中国农学通报,2013,29(4):76-82. [13]AJISAKA H,KUGINUKI Y,YUI S,et al.Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L.ssp.Pekinensis syn.Campestris L.) using bulked segregant analysis[J].Euphytica,2001,118:75-81. [14]LI X N,RAMCHIARY N,DHANDAPANI V,et al.Quantitative trait loci mapping in Brassica rapa revealed the structural and functional conservation of genetic loci governing morphological and yield component traits in the A，B，and C subgenomes of Brassica species[J].DNA Res,2013,20(1):1-16. [15]张磊.白菜抽薹相关性状QTL定位分析[D].沈阳:沈阳农业大学,2014. [16]JUNG C,MLLER A E.Flowering time control and applications in plant breeding[J].Trends Plant Sci,2009,14(10):563-573. [17]JARILLO J A,PINEIRO M.Timing is everything in plant development.The central role of floral repressors[J].Plant Science,2011,181(4):364-378. [18]李盛.春化处理对萝卜抽薹和开花的影响[D].洛阳:河南科技大学,2014. [19]ELERS B,WIEBE H J.Flower formation of Chinese cabbage.Response to vernalization and photoperiods[J].Scientia Horticulture,1984,22(3):219-231. [20]蒋兵涛.西藏大白菜抽薹影响因素及耐抽薹品种选育研究进展[J].现代农业科技,2016(1):127-128. [21]PRESSMAN E,SHAKCD R.Bolting and flowering of Chinese cabbage as affected by the intensity and source of supplementary light[J].Scientia Horticulturae,1988,34(4):177-187. [22]张鲁刚,孔小平,惠麦侠,等.大白菜幼苗春化对低温、光照和苗岭的要求[J].园艺学报,2008,35(11):1676-1680． [23]〖JP2〗MLLER-STEINBACH Y,ALEXANDRE C,HENNIG L.Flowering time control[M]//HENNIG L,KHLER C (eds.).Plant developmental biology，methods in molecular biology.London:Springer Science and Business Media,LLC,2010. [24]NAKAMA L,KATSURA N,KOSHIOKA M,et al.Role of endogenous gibberellins in cold-induced stem elongation and flowering of Japanese radish[J].Japan Soc Hortic Sci,1998,67:325-330. [25]侯雷平,王丽,李梅兰.赤霉素处理促进白菜开花的效应分析[J].中国农学通报,2009(6):171-174. [26]王淑芬,徐文玲.赤霉素对耐抽薹萝卜抽薹开花的影响[J].山东农业科学,2002(6):14-16. [27]MELZER S,KAMPMANN G,CHANDLER J,et al.〖STBX〗FPF1 〖STBZ〗modulates the competence to flowering in Arabidopsis[J].Plant J,1999,18(4):395-405. [28]赵丽萍.萝卜抽蔓性遗传分析与春萝卜种质标记鉴定[D].南京:南京农业大学,2007. [29]KANEKO Y,BANG S W.Early-bolting trait and RAPD markers in the specific monosomic addition of radish carrying type e-chromosome of Brassica oleracea[J].Plant Breeding,2000,119:137-140. [30]许园园,王燕,柳李旺,等.萝卜抽薹开花促进因子〖STBX〗ＲsFPF1〖STBZ〗基因克隆与功能分析[J].南京农业大学学报,2014,37(4):31-38. [31]YI G,PARK H,KIM J S,et al.Identification of three FLOWERING LOCUS C genes responsible for vernalization response in radish (Raphanus sativus L.)[J].Hort Environ Biotechnol,2014,55(6):548-556. [32]ZHANG L,JIA H,YIN Y,et al.Transcriptome analysis of leaf tissue of Raphanus sativus by RNA sequencing[J/OL].PLoS One,2013,8(11):e80350.doi:10.1371/journal.pone.0080350. [33]郭军,祖艳侠,吴永成,等.萝卜属抽薹开花相关基因FLC的预测及分析[J].浙江农业学报,2014,26(3):656-660. [34]王夏,孙菲菲,郦月红,等.萝卜抽薹开花相关基因的研究进展[J].金陵科技学院学报,2014,30(3):68-71. [35]NIE S,LI C,WANG Y,et al.Transcriptomic analysis identifies differentially expressed genes (DEGs) associated with bolting and flowering in radish (Raphanus sativus L.)[J].Frontiers in Plant Science,2016(7):1-12. [36]宋贤勇.萝卜花芽分化、抽薹与内源激素和DNA甲基化关系的初步研究[D].南京:南京农业大学,2006. [37]柳李旺,宋贤勇,龚义勤,等.萝卜MSAP体系优化与抽薹过程中MSAP分析[J].江苏农业科学,2006(6):203-206. [38]NIE S,XU L,WANG Y,et al.Identification of bolting-related microRNAs and their targets reveals complex miRNA-mediated flowering-time regulatory networks in radish (Raphanus sativus L.)[J].Scientific Reports,2015(5):1-15. [39]邓俭英.萝卜花芽分化形态发育及其生理生化的研究[D].杭州:浙江大学,2003. [40]刘可群,邱正明,聂启军,等.基于春化效应的高山萝卜生育期模拟模型研究[J].中国农学通报,2016,32(13):39-43. [41]YOSHIDA Y,TAKADA N,KODA Y.Isolation and identification of an anti-bolting compound，hexadecatrienoic acid monoglyceride，responsible for inhibition of bolting and maintenance of the leaf rosette in radish plants[J].Plant Cell Physiol,2010,51(8):1341-1349. [42]宋贤勇,柳李旺,龚义勤,等.春萝卜抽薹过程中内源激素含量变化分析[J].植物研究,2007(27):182-185. [43]王淑芬,徐文玲,何启伟,等.春化深度对萝卜抽薹的影响及抽薹过程中GA3和IAA含量的变化[J].山东农业科学,2003(6):20-21. [44]秦成萌,林多.不同植物生长调节剂抑制反季节萝卜抽薹效应的研究[J].北方园艺,2012(2):23-25. [45]张延恒,黄怡弘,刘海平.耐抽薹萝卜品种比较试验[J].浙江农业科学,2010(4):726-727. [46]汪炳良,邓俭英,曾广文.播种期和品种对萝卜现蕾、抽薹和开花的影响[J].种子,2003(3):15-17. [47]KONG Q,LI X,XIANG C,et al.Genetic diversity of radish (Raphanus sativus L.) germplasm resources revealed by AFLP and RAPD markers[J].Plant Mol Biol Rep,2011,29:217-223. [48]SHEN D,SUN H,HUANG M,et al.Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.)[J].BMC Genomics,2013(14):721. [49]王明霞.萝卜及其近缘属种亲缘关系分析与遗传图谱构建[D].南京:南京农业大学，2007. [50]方平,陈发波,姚启伦,等.肉质色不同萝卜遗传多样性的SSR分子标记分析[J].植物遗传资源学报,2012(2):226-232. [51]杨丽娟.硫代葡萄糖苷标记鉴定萝卜种质资源遗传多样性的研究[D].合肥:安徽农业大学,2010. [52]孔秋生.萝卜种质资源遗传多样性和亲缘关系的研究[D].武汉:华中农业大学,2003. [53]韩太利,李可峰,谭金霞.利用形态学与AFLP标记研究栽培萝卜种质亲缘关系[J].中国蔬菜,2008(9):15-18. [54]王夏,孙菲菲,王强.萝卜种质资源的遗传多样性和聚类分析[J].江西农业学报,2013,25(9):37-40. [55]宋倩.耐抽薹萝卜优质自交系的筛选及3种植物生长调节剂对抽薹的影响[D].武汉:华中农业大学,2013. [56 ]张秋萍,苏小俊,蔡善亚.棚室栽培冬春萝卜抗先期抽薹鉴定方法的技术要点[J].长江蔬菜,2016(15):37-38. [57]WANG Q,ZHANG L,ZHENG P.Genetic diversity and evolutionary relationship analyses within and among Raphanus species using EST-SSR markers[J/OL].Molecular Breeding,2015,35:62.doi:10.1007/s11032-015-0261-1. [58]李芳,徐良,魏美甜,等.萝卜IRAP技术体系建立与品种指纹图谱构建[J].江苏农业学报,2015,31(1):143-148. [59]孔秋生,李锡香,向长萍,等.沈镝栽培萝卜种质亲缘关系的AFLP分析[J].中国农业科学,2005，38(5):1017-1023. [60]孔秋生,李锡香,向长萍,等.萝卜种质资源亲缘关系的RAPD分析[J].植物遗传资源学报,2004,5(2):156-160. [61]周娜,李丹丹,陶伟林,等.萝卜种质遗传多样性的ISSR,RAPD与RAMP分析[J].西南农业学报,2015(28):704-711. [62]徐文玲,王淑芬,牟晋华,等.萝卜抽薹基因连锁的AFLP和SCAR分子标记鉴定[J].分子植物育种,2009,7(4):743-749. [63]樊志明.萝卜甘蓝双二倍体雄性不育性的遗传及细胞学研究[D].武汉:华中农业大学,2010. [64]蒋振,张晓辉,蒋磊,等.青花菜与萝卜属间杂种的表型和分子鉴定[J].植物遗传资源学报,2014,15(4):859-864. [65]BROWN G G,FORMANOVA N,JIN H,et al.The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats[J].Plant J,2003,35(2):262-272. [66]SCHNABLE P S,WISE R P.The molecular basis of cytoplasmic male sterility and fertility restoration[J].Trends Plant Sci,1998,3(5):175-180. [67]KOIZUKA N,IMAI R,FUJIMOTO H,et al.Genetic characterization of a pentatricopeptide repeat protein gene，〖STBX〗orf687〖STBZ〗，that restores fertility in the cytoplasmic male-sterile Kosena radish[J].Plant J,2003,34(4):407-415. [68]汪志伟.萝卜细胞质雄性不育胞质和核基因的分子标记的开发及其分子特征[D].武汉:华中农业大学,2006. [69]SUN X,LIU Y,WANG L,et al.Molecular characterization of the 〖STBX〗Rs-Rf1 〖STBZ〗gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration[J].Mol Breeding,2012,30:1727-1736. [70]谭婷婷,刘立锋,陈伟,等.利用分子标记鉴定萝卜细胞质雄性不育种质的研究[J].山东农业科学,2011(8):8-13. [71]胡天华,毛伟海,包崇来,等.耐抽薹萝卜雄性不育系CL09A的选育及利用[J].浙江农业科学,2015,56(9):1430-1432. [72]刘贤娴,徐文玲,刘辰,等.早春耐抽薹萝卜新品种“天正萝卜14 号”的选育[J].山东农业科学,2016,48(8):23-25.
相似文献/References:
[1]王 爽,林 多,杨延杰.萝卜芽苗菜品种筛选[J].北方园艺,2014,38(13):31.
WANG Shuang,LIN Duo,YANG Yan-jie.Screening of Radish Cultivars Suited for Sprout Vegetable Cultivation[J].Northern Horticulture,2014,38(07):31.
[2]杨金兰,郭 竞,刘艳波,等.秋露地萝卜新品种（系）比较试验[J].北方园艺,2013,37(22):39.
YANG Jin-lan,GUO Jing,LIU Yan-bo,et al.Comparative Trial of Open Field New Cultivars(Lines) of Autumn Radish[J].Northern Horticulture,2013,37(07):39.
[3]王文全,周 颖,阿依丁别克.再生水灌溉及污泥施肥对蔬菜种子发芽及幼苗生长的影响[J].北方园艺,2013,37(22):181.
WANG Wen-quan,ZHOU Ying,Ayidingbieke.Influence of Reclaimed Water Irrigation and Sludge Fertilizer on Seed Germination and Seedling Growth of Vegetables[J].Northern Horticulture,2013,37(07):181.
[4]杨延杰,林多.邻苯二甲酸对蔬菜种子萌发和幼苗生长及根系形态的影响[J].北方园艺,2013,37(13):9.
YANG Yan-jie,LIN Duo.Effects of Phthalic Acid on Seed Germination，Seedling Growth and Root Morphology of Cucumber and Radish[J].Northern Horticulture,2013,37(07):9.
[5]张晓梅,苗增建.西宁地区温室大棚秋茬萝卜新品种比较试验[J].北方园艺,2013,37(14):61.
ZHANG Xiao-mei,MIAO Zeng-jian.Variety Comparative Test of Radish New Variety in Greenhouse in Xining Region[J].Northern Horticulture,2013,37(07):61.
[6]张 丽,郑鹏婧.春白萝卜游离小孢子培养的研究[J].北方园艺,2013,37(23):31.
ZHANG Li,ZHENG Peng-jing.Isolated Microspore Culture of Spring-white Radish (Raphanus sativus L.var. longpinnatus Bailey)[J].Northern Horticulture,2013,37(07):31.
[7]李泸,韩明蓉.大白菜新品种“双冠王”的选育[J].北方园艺,2014,38(06):159.
LI Lu,HAN Ming-rong.Breeding of A New Chinese Cabbage Cultivar ‘Shuangguan Wang’[J].Northern Horticulture,2014,38(07):159.
[8]孙丽,李贞霞,原让花,等.微波消解ICP-AES法测定萝卜肉质根矿质元素研究[J].北方园艺,2013,37(11):34.
SUN Li,LI Zhen-xia,YUAN Rang-hua,et al.Study on Determination of Mineral Elements in Fleshy Root of Radish by Microwave Digestion ICP-AES Method[J].Northern Horticulture,2013,37(07):34.
[9]袁伟玲,梅时勇,崔磊,等.萝卜主要农艺性状与肉质根产量的相关性及通径分析[J].北方园艺,2013,37(11):37.
YUAN Wei-ling,MEI Shi-yong,CUI Lei,et al.Correlation and Path Analysis of the Major Agronomic Characters of ?Radish and Fleshy Root Yield[J].Northern Horticulture,2013,37(07):37.
[10]秦成萌,林多.不同植物生长调节剂抑制反季节萝卜抽薹效应的研究[J].北方园艺,2012,36(02):23.
QIN Cheng-meng,LIN Duo.Study on Depression Effects of Anti-season Radish Cultivation with Different Plant Growth Regulators Treatments[J].Northern Horticulture,2012,36(07):23.
备注/Memo
第一作者简介：张万萍（1970-），女，博士，教授，现主要从事蔬菜遗传育种与栽培管理等研究工作。E-mail：zwp269@126.com.基金项目：贵州省科技厅十二五重大专项资助项目（黔科合重大专项字[2013]6007号）。