DONG Yuanyuan,LI Xiaowei,YAO Na,et al.Transcription Factor Identification and Analysis in Leymus chinensis Transcriptome[J].Northern Horticulture,2017,41(07):115-120.[doi:10.11937/bfyy.201707024]
盐碱胁迫下羊草转录因子的转录组分析
- Title:
- Transcription Factor Identification and Analysis in Leymus chinensis Transcriptome
- Keywords:
- transcriptome; Leymus chinensis; transcription factor; saline-alkaline stress; high-sequencing
- 文献标志码:
- A
- 摘要:
- 以羊草为试材，采用罗氏454二代测序方法，研究了羊草转录因子家族在逆境胁迫条件下来自38个家族的243个转录因子表达差异模式，以期揭示WRKY、C3HL、NAC等转录因子家族差异基因数量，探讨羊草的抗逆分子机制。结果表明：羊草转录组测序片段经de novo拼接共获得104 105条Unigene序列，对照含有97个转录因子基因，盐碱处理组含有213个转录因子，其中67个转录因子为2组共表达。差异基因表达分析揭示羊草主要通过WRKY、C3HL、NAC转录因子家族的差异表达调控盐碱适应性。该研究为耐盐碱型作物培育奠定了基础。
- Abstract:
- Leymus chinensis (Trin.) Tzvel.plays an important role in protection of natural environment,Roche-454 massive parallel pyrosequencing technology was employed to identify transcription factor,and gene expression in the saline-alkaline stress response of L.chinesis.The results indicated that a total of 104 105 Unigenes were obtained.243 transcription factors belong to 38 transcription factor family were obtained by BLASTx according to Nr database.Among them,97 TFs were obtained in control sample,213 TFs were obtained in saline-alkaline stress sample and 67 TFs were co-expressed in both samples.Compared with control,138 TFs were differently expressed.Data analysis revealed that different expressed WRKY,C3HL,NAC induced saline-alkali response in leaves of L.chinesis.The basis for further exploring molecular response mechanism to saline-alkali stress in Leymus chinensis was established.
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备注/Memo
第一作者简介：董园园（1985-），女，博士，讲师，现主要从事植物分子生物学等研究工作。E-mail：yydong@aliyun.com.责任作者：李海燕（1971-），女，博士，教授，博士生导师，现主要从事抗逆分子生物学等研究工作。E-mail：hyli99@163.com.基金项目：农业部转基因生物新品种培育重大专项资助项目（2016ZX08010002）；国家自然科学基金资助项目（31271746，31101091，31501366）；吉林省科技厅资助项目（20140520164JH，20150623024TC-11）；吉林农业大学科研启动基金资助项目（2015018）。