ZENG Bin,LIU Mengwen,WANG Jianyou,et al.Molecular Identification Analysis of Six New S-RNase Genes Controlling Self-incompatibility From Xinjiang Wild Almond（Prunus tenella Batsch.）[J].Northern Horticulture,2017,41(07):105-115.[doi:10.11937/bfyy.201707023]
新疆野扁桃六个新S-RNase基因的克隆和序列分析
- Title:
- Molecular Identification Analysis of Six New S-RNase Genes Controlling Self-incompatibility From Xinjiang Wild Almond（Prunus tenella Batsch.）
- 关键词:
- 新疆野扁桃; 自然居群; 自交不亲和性; S-RNase基因; 克隆鉴定
- Keywords:
- Prunus tenella; natural population; self-incompatibility; S-RNase gene; clone and identification
- 文献标志码:
- A
- 摘要:
- 以我国闻名于世的珍稀野生果树新疆野扁桃为试材，利用蔷薇科果树自交不亲和性通用引物组合，采用S-RNase等位基因PCR扩增，RT-PCR和RACE等技术方法，克隆了新疆野扁桃不同株系的S-RNase基因，以期为新疆野扁桃自交不亲和分子机理的进一步研究提供基本的资料。结果表明：从新疆野扁桃不同株系中克隆鉴定出了6个新的S-RNase基因，分别命名为PteS10(GeneBank登陆号：KJ755352)，PteS11(GeneBank登陆号：KJ755353)，PteS12(GeneBank登陆号：KJ755354)，PteS13(GeneBank登陆号：KJ755355)，PteS14(GeneBank登陆号：KJ755356)和PteS15(GeneBank登陆号：KJ755357)；并对这6个新基因进行了序列生物信息学分析，为基于新疆野扁桃自交不亲和特性的遗传改良以及分子调控模式奠定试验基础。
- Abstract:
- TakingPrunus tenella Batsch.(wild almond) as materials,which is a very primitive tree species,precious,ancient and very scarce in the world today.The use of Rosaceae self-incompatibility primer combinations, using S-RNase allele PCR amplification,RT-PCR and RACE technology,the S-RNase gene of Xinjiang wild almond was studied,in order to further study for the Xinjiang wild almond molecular mechanism of self-incompatibility.The results showed that six new self-incompatibility S-RNase genes were successfully cloned from the pistils of the wild almond from five natural populations different individuals sample,which were named PtS10 (GeneBank accession number：KJ755352),PtS11 (GeneBank accession number：KJ755353),PtS12 (GeneBank accession number：KJ755354),PtS13 (GeneBank accession number：KJ755355),PtS14 (GeneBank accession number：KJ755356) and PtS15(GeneBank accession number：KJ755357)；and the six new genes were analyzed by bioinformatics study.These studies provided basic information for the further study of molecular mechanism of self-incompatibility in Xinjiang wild almond.
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备注/Memo
第一作者简介：曾斌（1969-），男，博士，副教授，现主要从事果树种质资源及生物技术的教学与科研等工作。E-mail：zbxnd@163.com.基金项目：国家自然科学基金资助项目（31660557，31260465）；新疆维吾尔自治区果树学重点学科资助项目（201007）。