DANG Yuejia,BAI Lingling,ZHANG Yanju,et al.SSR Genetic Diversity Analysis of Pseudoperonospora cubensis[J].Northern Horticulture,2017,41(06):130-134.[doi:10.11937/bfyy.201706030]
黄瓜霜霉病菌SSR遗传多样性分析
- Title:
- SSR Genetic Diversity Analysis of Pseudoperonospora cubensis
- Keywords:
- Pseudoperonospora cubensis; SSR; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以来自黑龙江省、吉林省、辽宁省、北京、山东省、江苏省、湖北省和广东省8个省份的36株黄瓜霜霉菌为试材，采用SSR分子标记的方法，研究了黄瓜霜霉病菌的遗传多样性。结果表明：中国黄瓜霜霉菌遗传分化较大，群体遗传多样性比较丰富。利用11对SSR引物共扩增出170条谱带，其中多态性条带160条，占总带数的94.1%。从11对引物中筛选出了多态性好、条带清晰的引物D13。通过NTSYS软件构建了黄瓜霜霉菌的亲缘关系树状图，聚类分析结果表明，菌株间的相似系数在0.43~1.00，说明SSR遗传多样性与菌株的地理来源存在一定的相关性。
- Abstract:
- Thirty-six isolates of cucumber downy mildew from Heilongjiang,Jilin,Liaoning,Beijing,Shandong,Jiangsu,Hubei,and Guangdong Province were amplified.Using SSR molecular marker method studied the SSR genetic diversity analysis of Pseudoperonospora cubensis.The results showed that China had bigger genetic differentiation,and abundant genetic diversity of Pseudoperonospora cubensis.In total of 170 bands were obtained,including 160 polymorphic bands accounting for 94.1%.D13 were screened as the best polymorphic primer from 11 primers.The NTSYS software was used to build the relationship dendrogram of P.cubensis,cluster analysis showed that the coefficient of similarity was from 0.43 to 1.00.There were certain correlations between SSR genetic diversity and geographical origin.
参考文献/References:
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备注/Memo
第一作者简介：党悦嘉（1991-），女，硕士研究生，研究方向为植物病原生物学。E-mail：978836015@qq.com.责任作者：张艳菊（1968-），女，博士，教授，现主要从事植物病原生物学及有害生物综合治理等研究工作。E-mail：zhangyanju1968@163.com.基金项目：国家自然科学基金资助项目（31171792）；黑龙江省自然科学基金重点资助项目（ZD2016003）。