[1]杜艳,王娟,陈冲,等.耧斗菜野生资源的AFLP遗传多样性分析[J].北方园艺,2017,41(05):85-88.[doi:10.11937/bfyy.201705020]
　DU Yan,WANG Juan,CHEN Chong,et al.Genetic Diversity of Wild Resources of Aquilegia Based on AFLP Analysis[J].Northern Horticulture,2017,41(05):85-88.[doi:10.11937/bfyy.201705020]
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耧斗菜野生资源的AFLP遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年05 页码: 85-88 栏目: 出版日期: 2017-03-15
Title:
Genetic Diversity of Wild Resources of Aquilegia Based on AFLP Analysis
作者:
杜艳1; 王娟2; 陈冲2; 段莎莎3
（1.山西运城农业职业技术学院 农林与工程系，山西 运城 044000；2.山西省农业科学院 果树研究所，山西 太原 030031；3.西南科技大学 生命科学与工程学院，四川 绵阳 621010）
Author(s):
DU Yan1; WANG Juan2; CHEN Chong2; DUAN Shasha3
(1.Department of A&F Engineering，Shanxi Yuncheng Vocational and Technical College of Agricultural,Yuncheng，Shanxi 044000;2.Pomology Institute，Shanxi Academy of Agricultural Sciences，Taiyuan，Shanxi 030031；3.School of Life Science and Engineering，Southwest University of Science and Technology，Mianyang，Sichuan 621010)
关键词:
耧斗菜; AFLP; 遗传多样性
Keywords:
Aquilegia; AFLP; genetic diversity
DOI:
10.11937/bfyy.201705020
文献标志码:
A
摘要:
以包括22份野生资源的5个耧斗菜物种为试材，采用AFLP分子标记方法，分析了中国耧斗菜野生资源的遗传变异和遗传多样性，为系统鉴定耧斗菜野生资源奠定基础。结果表明：10对引物共扩增出293个多态性位点，多态性位点比率为62.5%。Shannon多样性和He值分析结果表明，5个物种的遗传多样性大小顺序为无距耧斗菜>耧斗菜>尖萼耧斗菜>直距耧斗菜>华北耧斗菜。遗传距离分析结果表明，耧斗菜与尖萼耧斗菜遗传距离最小，尖萼耧斗菜与直距耧斗菜遗传距离最大。
Abstract:
In order to systematic identify genetic diversity of wild resources of Aquilegia in China，five species of Aquilegia including 22 wild resources were investigated by AFLP markers method.The results showed that a total of 293 polymorphic fragments were produced by 10 AFLP primers，which accounted for 62.5% of the total fragments.The genetic diversity by Shannon index and He index indicated that the highest genetic diversity species was A.ecalcarata，and followed by gradual decrease as A.viridiflora,A.oxysepala，A.rockii，and A.yabeana.The genetic distance analysis indicated that A.oxysepala and A.viridiflora showed the shortest genetic distance，whereas A.oxysepala and A.rockii showed the longest distance.

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备注/Memo

第一作者简介：杜艳（1980-），女，山西运城人，硕士，讲师，现主要从事花卉植物栽培与应用等研究工作。E-mail:xudu20061@163.com.基金项目：国家自然科学基金资助项目(31400131,81603223)。

更新日期/Last Update: 2017-03-24