TANG Yuhai,QIAO Ning,SUN Xiaohui.Identification and Sequence Analysis of Shouguang Isolate by RT-PCR of Pepper Mild Mottle Mosaic Virus[J].Northern Horticulture,2017,41(04):94-97.[doi:10.11937/bfyy.201704021]
辣椒轻斑驳花叶病毒寿光分离物的RT-PCR检测及其序列分析
- Title:
- Identification and Sequence Analysis of Shouguang Isolate by RT-PCR of Pepper Mild Mottle Mosaic Virus
- 文献标志码:
- A
- 摘要:
- 以山东寿光地区感染辣椒轻斑驳花叶病毒的辣椒为试材，采用试剂盒提取感病辣椒的总RNA并进一步反转录成cDNA，参照已报道的PMMoV检测引物合成特异性引物PMMoV-F和PMMoV-R；以获得的cDNA为模板进行PCR扩增，研究了辣椒轻斑驳花叶病毒寿光分离物。结果表明：得到分子量约是576 bp的条带，纯化后进行基因测序；通过测序结果分析比对可知，PMMoV寿光分离物序列与诸多地区的分离物同源性较高，可达到99%~100%。
- Abstract:
- Peppers infected by PMMoV in Shouguang city,Shandong Province were used as test material and kit was used to extract total RNA from infected pepper and total RNA was further reversed into cDNA by reverse transcription.PMMoV-F and PMMoV-R was synthesized according to reported specific primers.The cDNA of PMMoV isolate was used for PCR amplication.The results showed that the molecular weight of band was 576 bp.Sequence analysis showed that PMMoV infected pepper in Shouguang had closer genetic relationship with PMMoV isolated in other areas，which could achieve 99%-100%.
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备注/Memo
第一作者简介：唐玉海(1982-)，男，博士，讲师，现主要从事蔬菜栽培生理及分子生物学等研究工作。E-mail:tangyh209@163.com.责任作者：乔宁（1978-），男，硕士，讲师，现主要从事蔬菜育种及病虫害防治等研究工作。E-mail：304754522@qq.com.基金项目：公益性行业（农业）科研专项资助项目（201303028）；山东省自然科学基金资助项目（ZR2012CM032）；山东省科技发展计划资助项目（2010GNC10915）。