DU Bin.Identification of Antagonistic Strain Against Cucumber Fusarium Wilt and Optimization of Its Fermentation Medium[J].Northern Horticulture,2017,41(03):132-137.[doi:10.11937/bfyy.201703030]
一株黄瓜枯萎病拮抗菌的鉴定及发酵培养基的优化
- Title:
- Identification of Antagonistic Strain Against Cucumber Fusarium Wilt and Optimization of Its Fermentation Medium
- 文献标志码:
- A
- 摘要:
- 采用形态观察和生理生化特性检测方法，结合16S rRNA基因序列分析，将一株黄瓜枯萎病拮抗菌株KC24鉴定为类芽孢杆菌，并对其进行了抑菌谱测试。结果表明：KC24能拮抗多种植物病原菌，具有广谱抗菌活性，利用响应面法对其产芽孢发酵培养基进行了优化，优化后的发酵液中芽孢数量达13.5×108 cfu?mL-1，比优化前提高了107.7%。最优产孢培养基配方为玉米粉20 g，豆粕5 g，KNO3 2.0 g，KH2PO4 2.59 g，NaCl 1.0 g，(NH4)2SO4 2.04 g，FeSO4 0.009 1 g，MgSO4?7H2O 1 g，H2O 1 L，pH 7.0。
- Abstract:
- Based on the morphological，physiological and 16S sRNA sequence characteristics，a bacterial strain was identified as Paenibacillus，named KC24.Bacteriostatic spectrum of KC24 was detected.The results showed that KC24 could confront a variety of plant pathogens，it had a broad-spectrum antimicrobial activity.The fermentation medium components for spore production of KC24 was optimized by responsesurface methodology.The optimized number of spores in the fermented liquid was 13.5×108 cfu?mL-1，increased more than 107.7%.The optimal fermentation conditions were corn flour 20 g，soybean meal 5 g，KNO3 2.0 g，KH2PO4 2.59 g，NaCl 1.0 g，(NH4)2SO4 2.04 g，FeSO4 0.009 1 g，MgSO4?7H2O 1 g，H2O 1 L，pH 7.0.
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备注/Memo
作者简介：杜宾（1980-），男，山西太原人，硕士，讲师，研究方向为植物保护。E-mail：weiapple001@126.com.