ZHU Jianmin,ZHANG Guoying,DOU Peipei,et al.Three-wavelength Ultraviolet Spectrophotometric Determination of Total Flavonoes From Cordyceps kyushuensis Extracted by Supercritical Fluid Method[J].Northern Horticulture,2017,41(02):132-135.[doi:10.11937/bfyy.201702032]
三波长法测定超临界萃取九州虫草总黄酮含量
- Title:
- Three-wavelength Ultraviolet Spectrophotometric Determination of Total Flavonoes From Cordyceps kyushuensis Extracted by Supercritical Fluid Method
- 关键词:
- 九州虫草; 黄酮类化合物; 三波长-紫外分光光度法; 超临界萃取
- Keywords:
- Cordyceps kyushuensis; flavone; three-wavelength ultraviolet spectrophotometry; supercritical fluid extraction
- 文献标志码:
- A
- 摘要:
- 以九州虫草为试材，采用三波长-紫外分光光度法，测定了九州虫草超临界流体萃取物中的总黄酮含量。结果表明：以芦丁为标准品，选定244、272、302 nm为测定波长，标准曲线回归方程为y=0.025 3x-0.090 4（r2=0.999 7）；在浓度16~56 μg?mL-1内，△A与浓度之间呈良好的线性关系，表明该方法稳定性好，回收率为94.80%～97.75%，精密度RSD<0.67%（n=6），超临界提取获得的虫草黄酮含量为1.89 mg?g-1。
- Abstract:
- Cordyceps kyushuensis was used as test material，three-wavelength ultraviolet spectrophotometry was used for determining the content of total flavones in Cordyceps kyushuensis which was extracted by Supercritical fluid method (SFE).The results showed that regression equation of ΔA rutin standard concentration was obtained：y=0.025 3x-0.090 4 with a relation coefficient r2=0.999 7.Rutin concentrations in 16-56 μg?mL-1 with ΔA obeyed the linear relation measured at wavelength λ1=302 nm，λ2=272 nm and λ3=244 nm.It indicated that the method had a good stability.The recovery was 94.80%-97.75%；the relative standard deviation was less than 0.67% (n=6).The content of total flavones extracted from Cordyceps kyushuensis by SFE was 1.89 mg?g-1.
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备注/Memo
第一作者简介：朱建民(1963-)，男，本科，高级讲师，研究方向为天然活性组分分析与工业生产。E-mail:82348177@qq.com.责任作者：凌建亚(1975-)，男，博士，教授，博士生导师，现主要从事药用真菌分子系统学及天然活性组分等研究工作。E-mail：lingjian-ya@sdu.edu.cn.基金项目：国家自然科学基金资助项目（30770041，30970012）；山东省自然科学基金资助项目（ZR2015CM028）；山东省科技攻关计划资助项目（2009GG10002063）。