[1]龙月红,宋菊,国红玉,等.刺五加转录因子基因的挖掘[J].北方园艺,2017,41(20):153-156.[doi:10.11937/bfyy.20170195]
　LONG Yuehong,SONG Ju,GUO Hongyu,et al.Exploring Transcription Factors in Eleutherococcus senticosus[J].Northern Horticulture,2017,41(20):153-156.[doi:10.11937/bfyy.20170195]
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刺五加转录因子基因的挖掘

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 153-156 栏目: 出版日期: 2017-10-30
Title:
Exploring Transcription Factors in Eleutherococcus senticosus
作者:
龙月红; 宋菊; 国红玉; 尤鹏升; 邢朝斌
华北理工大学 生命科学学院，河北 唐山 063000
Author(s):
LONG Yuehong; SONG Ju; GUO Hongyu; YOU Pengsheng; XING Zhaobin
College of Life Sciences,North China University of Science and Technology,Tangshan，Hebei 063000
关键词:
刺五加; 转录组; 转录因子; 皂苷
Keywords:
Eleutherococcus senticosus; transcriptome; transcription factors; saponins
DOI:
10.11937/bfyy.20170195
文献标志码:
A
摘要:
以刺五加嫩叶为试材，采用二代测序方法Illumina HiSeq 4000进行转录组测序和生物信息学方法，找出其转录因子，并随机挑选4个不同家族的转录因子，针对6个生长时期和3个器官的样本，使用实时荧光定量PCR确认转录因子的正确性。结果表明：刺五加转录组共计8.34 Gb，拼接得到77 087条Unigenes，有485个转录因子，分类于36个家族。通过qRT-PCR分析，Unigene 49771、Unigene 22314、Unigene 13139和Unigene 18837在不同生长发育时期和器官中表达差异极显著(P<0.01）。
Abstract:
The leaves of Eleutherococcus senticosus were used as experimental materials,the high-throughput sequencing technology (Illumina HiSeq 4000) was used to sequence the transcriptome，in order to find transcription factors from the transcriptome with the bioinformatic way.And then the qRT-PCR was used to analysis correctness of transcription factors with 6 kinds of stages and 3 kinds of tissues of E.senticosus.The results showed that the transcriptome of E.senticosus was 8.34 Gb,and it contained 77 087 Unigenes.There were 485 transcription factors in it,which involved in 36 families.By qRT-PCR analysis,the difference of Unigene 49771,Unigene 22314,Unigene 13139 and Unigene 18837 was highly significant in different growth stages and organs (P<0.01).

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备注/Memo

第一作者简介：龙月红(1974-），女，本科，实验师，研究方向为分子生药学。E-mail:lyhheuu@126.com.

责任作者：邢朝斌(1975-），男，硕士，教授，研究方向为分子生药学及药用植物细胞工程。E-mail：xzbheuu@126.com.
基金项目：国家自然科学基金资助项目(31570683）；河北省教育厅资助科研资助项目(QN2014102）；华北理工大学培育基金资助项目(SP201508）。
收稿日期：2017-04-05

更新日期/Last Update: 2017-11-02