[1]鲁海菊,董慧婷,祁建珉,等.抗扑海因枇杷内生木霉菌株PY2与原始菌株Y2生物学特性差异分析[J].北方园艺,2017,41(18):1-6.[doi:10.11937/bfyy.20170140]
　LU Haiju,DONG Huiting,QI Jianmin,et al.Differential Analysis for Biological Characteristics of Endophytic Trichoderma PY2 Strain Resistance to Iprodione and Wild Y2 Strain From Loquat[J].Northern Horticulture,2017,41(18):1-6.[doi:10.11937/bfyy.20170140]
点击复制

抗扑海因枇杷内生木霉菌株PY2与原始菌株Y2生物学特性差异分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年18 页码: 1-6 栏目: 出版日期: 2017-09-30
Title:
Differential Analysis for Biological Characteristics of Endophytic Trichoderma PY2 Strain Resistance to Iprodione and Wild Y2 Strain From Loquat
作者:
鲁海菊1; 董慧婷1; 祁建珉2; 王传铭1; 曾垄钢3
1.红河学院 生命科学与技术学院，云南 蒙自 661199；2.屏边县茶果站，云南 屏边 661299；3.屏边县植保植检站，云南 屏边 661299
Author(s):
LU Haiju1; DONG Huiting1; QI Jianmin2; WANG Chuanming1; ZENG Longgang3
1.Department of Life Science and Technology,Honghe College，Mengzi，Yunnan 661199;2.Pingbian Tea and Fruit Station，Pingbian，Yunnan 661299;3.Pingbian Station of Plant Protection and Quarantine，Pingbian，Yunnan 661299
关键词:
木霉; 抗扑海因; 生物学特性
Keywords:
Trichoderma; resistance to iprodione; biological characteristics
DOI:
10.11937/bfyy.20170140
文献标志码:
A
摘要:
以抗扑海因枇杷内生木霉PY2菌株和原始菌株Y2为研究对象，采用菌丝生长速率及血球计数法，研究了不同培养基、碳氮源、pH、温度和光照对其菌丝生长及产孢的影响,以期探索二者生物学特性差异。结果表明：2个菌株最大的差异在于产孢性状不同，抗扑海因菌株PY2产孢能力明显强于原始菌株Y2。PY2菌株菌丝生长最佳条件为PDA、CDA或BDA培养基，葡萄糖，酵母膏，pH 5、6、8、9、10，温度为15~28 ℃，全黑暗或全光照；其产孢最佳条件为CDA培养基，D-果糖，牛肉膏，pH 3，25 ℃，全光照。
Abstract:
In order to make clear difference of biological characteristics of Trichoderma PY2 resistant to iprodione and wild Y2 strain from loquat，the effects of medium，carbon and nitrogen source，pH，temperature and illumination on mycelial growth and sporulation quantity for PY2 and Y2 strains were studied by method of mycelial growth rate and cell count technique.The results showed that characteristic of generating spores was significantly different between PY2 and Y2.PY2 was more powerful than Y2 in ability of generating spores.The proper conditions of mycelial growth were mediums of PDA,CDA or BDA，glucose，yeast extract and pH 5,6,8,9,10，15-28 ℃，all dark or all light.The proper conditions of generating spores were medium of CDA，D-fructose，beef extract，pH 3，25 ℃ and all light.

参考文献/References:

[1]孟昭赫.介绍一种新的杀菌剂—扑海因(rovral)[J].环境卫生学杂志,1986(2):89-90.

[2]杨合同,唐文华,RYDER M.木霉菌与植物病害的生物防治[J].山东科学,1999,12(4):7-15,20.
[3]腾安娜.木霉菌对植物的促生效果及其机理的研究[D].济南:山东师范大学,2010:1-85.
[4]李卫平,林福呈.绿色木霉对蔬菜苗期病害的防治和促生作用[J].江苏农业学报,2000,12(2):106-107.
[5]刘云龙,何永红,张旭东.哈茨木霉对辣椒生长的影响[J].云南农业大学学报,2002,17(4):345-346.
[6]曾华兰,叶鹏盛,李琼芳,等.哈茨木霉T23对花生的促生增产作用[J].云南农业大学学报,2005,20(1):145-146.
[7]贾丙志.多功能木霉对纤维素和农药的降解特性研究[D].济南:山东师范大学,2010:1-63.
[8]张曼曼,邓春生,马金奉.多功能木霉的筛选及鉴定[J].农业环境科学学报,2012，31(8):1571-1575.
[9]于雪云.土壤中多功能木霉菌的筛选鉴定及木霉多样性分析[D].淄博:山东理工大学,2007:1-98.
[10]鲁海菊,刘云龙,张云霞,等.哈茨木霉多菌灵耐药性菌株的筛选[J].云南农业大学学报,2005,20(3):436-437.
[11]王勇,杨秀荣,刘水芳.拮抗木霉耐药性菌株的筛选及其与速克灵防治灰霉病的协调作用[J].天津农学院学报,2002,9(4):19-22.
[12]尹婷.深绿木霉T2对微生物种群数量的影响及抗药性菌株的筛选[D].兰州:甘肃农业大学,2012:1-65.
[13]付文祥.有机磷农药降解菌木霉FM10的生长条件研究[J].生物磁学,2005,5(3):29-31.
[14]刘新,尤民生，魏英智,等.木霉Y对毒死蜱和甲胺磷的降解作用[J].福建农林大学学报,2002,31(4):455-458.
[15]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998:57-125.
[16]段银芝,郎剑锋,孔凡彬,等.4种农药对哈茨木霉生长的影响[J].江苏农业科学,2013,41(3):101-102.
[17]田连生,陈菲.木霉菌剂与多菌灵协调防治灰霉病试验[J].江苏农业科学,2013,41(12):132-133.
[18]任凤山,王燕,翟一凡,等.木霉与几种化学杀菌剂协调防治苹果轮纹病[J].北方园艺,2015(16):111-115.

相似文献/References:

[1]高苇,李宝聚,王万立,等.菌糠木霉发酵物对黄瓜枯萎病的防效研究[J].北方园艺,2013,37(16):140.
　GAOWei,LIBao-ju,WANGWan-li,et al.StudyonControlEffectof Trichoderma spp-spentMushroomSubstrateFermentationto CucumberFusarium Wilt[J].Northern Horticulture,2013,37(18):140.
[2]古丽吉米拉·米吉提,王志英,王 娜,等.木霉菌的生物防治分子机理[J].北方园艺,2013,37(23):206.
　MIJITI Gulijimila,WANG Zhi-ying,WANG Na,et al.Molecular Mechanism of Biological Control of Trichoderma spp.[J].Northern Horticulture,2013,37(18):206.
[3]张立恒,李 坤,胡熙禧,等.接种微生物菌剂对葡萄连作土壤速效养分及酶活性的影响[J].北方园艺,2015,39(04):162.[doi:10.11937/bfyy.201504040]
　ZHANG Li-heng,LI Kun,HU Xi-xi,et al.Effect of Microbial Agents on the Available Nutrient and Enzyme Activity of Grape Replant Soil[J].Northern Horticulture,2015,39(18):162.[doi:10.11937/bfyy.201504040]
[4]任凤山,王燕,翟一凡,等.木霉与几种化学杀菌剂协同防治苹果轮纹病[J].北方园艺,2015,39(16):111.[doi:10.11937/bfyy.201516026]
　REN Fengshan,WANG Yan,ZHAI Yifan,et al.Synergistic Effect of Trichoderma and Several Chemical Fungicides for Control of Botryosphaeria dothidea[J].Northern Horticulture,2015,39(18):111.[doi:10.11937/bfyy.201516026]
[5]胡 琼.木霉对植物促生作用的研究进展[J].北方园艺,2010,34(07):0.[doi:10.11937/bfyy.201007077]
　HU Qiong.Advancement on the Research of the Trichoderma Promotion to Plant Growth[J].Northern Horticulture,2010,34(18):0.[doi:10.11937/bfyy.201007077]
[6]周红姿,李宝聚刘开启.抗药性木霉菌研究进展[J].北方园艺,2003,27(06):0.[doi:10.11937/bfyy.200306005]
　[J].Northern Horticulture,2003,27(18):0.[doi:10.11937/bfyy.200306005]
[7]夏海波,潘好芹,李艳青.拮抗蔬菜土传病害木霉菌株的筛选及鉴定[J].北方园艺,2017,41(06):127.[doi:10.11937/bfyy.201706029]
　XIA Haibo,PAN Haoqin,LI Yanqing.Screening and Identification of Antagonistic Trichoderma Strains Against Soil-born Vegetable Pathogens[J].Northern Horticulture,2017,41(18):127.[doi:10.11937/bfyy.201706029]
[8]张璐,杨瑞秀,王莹,等.甜瓜连作土壤中酚酸类物质测定及降解研究[J].北方园艺,2017,41(09):14.[doi:10.11937/bfyy.201709004]
　ZHANG Lu,YANG Ruixiu,WANG Ying,et al.Identification and Degradation of Phenolic Acids in Rhizosphere Soil of Replanted Muskmelon[J].Northern Horticulture,2017,41(18):14.[doi:10.11937/bfyy.201709004]
[9]周畅,王金杰,范海娟,等.稠李根围木霉的分离及鉴定[J].北方园艺,2018,42(12):88.[doi:10.11937/bfyy.20172427]
　ZHOU Chang,WANG Jinjie,FAN Haijuan,et al.Separation and Identification of Trichoderma From the Rhizosphere Soil of Prunus padus[J].Northern Horticulture,2018,42(18):88.[doi:10.11937/bfyy.20172427]
[10]刘昆昂,张根伟,马宏,等.抗木霉白色金针菇菌株的选育及其栽培配方[J].北方园艺,2019,43(01):150.[doi:10.11937/bfyy.20181758]
　LIU Kunang,ZHANG Genwei,MA Hong,et al.Breeding and Cultivation Formula of Flammulina velutipes of anti-Trichoderma harzianum[J].Northern Horticulture,2019,43(18):150.[doi:10.11937/bfyy.20181758]

备注/Memo

第一作者简介：鲁海菊（1978-），女，博士，教授，现主要从事植物真菌病害及其生物防治等研究工作。E-mail：luhaiju2011@126.com.

基金项目：红河学院应用型科学研究重点资助项目（XJY15Z06）；国家自然科学基金资助项目（31660147）；云南省应用基础研究计划资助项目（2016FB066）。
收稿日期：2017-03-31

更新日期/Last Update: 2017-09-30