WANG Yunsheng,WEN Guangqin,NIE Fei.Leaf Transcriptome Sequencing and Bioinformatics Analysis of Lowbush Blueberry Variety ‘Blomidon’[J].Northern Horticulture,2017,41(01):101-107.[doi:10.11937/bfyy.201701023]
矮丛蓝莓品种“美登”叶片转录组测序和生物信息学分析
- Title:
- Leaf Transcriptome Sequencing and Bioinformatics Analysis of Lowbush Blueberry Variety ‘Blomidon’
- 文献标志码:
- A
- 摘要:
- 以矮丛蓝莓品种“美登”为试材，采用第二代测序技术对其嫩叶转录组进行了测序及生物信息学分析，研究了矮丛蓝莓功能基因组信息，以期增加对矮丛蓝莓生长发育分子机理的了解，并为矮丛蓝莓的分子标记辅助育种奠定基础。结果表明：在获得的约1 G纯净数据，共拼装了41 929条Unigenes，其中被GO数据库成功注释Unigene分别涉及到生物学过程、细胞成分及分子功能相关的共45类生理代谢功能；KOG数据库注释到19 149条Unigenes，共涉及25条代谢通路；KEGG数据库成功注释13 072条Unigenes，共涉及到5个大类、18个中类、129条代谢通路；Nr数据库成功注释30 738条Unigenes，这些Unigenes共对应32 949条蛋白质的编码区。同时，生物信息学分析还显示，全部Unigenes中，有884条编码转录因子，涉及到55个家族；2 261条编码抗性基因，涉及18个家族；检测到6 591个SSR位点，其中2碱基重复的SSR数目最多，占总SSR位点数的70%。
- Abstract:
- In the hope of increasing the understanding of molecular mechanism of the growth and development of Vaccinium angustifolium，and establishing the foundation for the molecular marker assisted breeding of dwarf blueberry，cultivar ‘Blomidon’ of dwarf blueberry was selected as test material，and its young leave transcriptome was sequenced by the second sequencing technology and then was made bioinformatics analysis，the aim was to study the functional genomic information of lowbush blueberry.The results showed that,total about 1 G clean reads could been assembled into 41 929 Unigenes.Among them，Unigenes were involving 45 kinds metabolism and physiological function of biological process，cellular component and molecular function according to the annotation results of dataset GO；19 149 Unigenes could been annotated by KOG dataset，which were involving in 25 metabolism pathways；13 072 Unigenes cauld been annotated by KEGG dataset，which were involving in five major categories，18 middle categories，and 129 metabolism pathways；30 738 Unigenes could been annotated by dataset Nr，which were mapping into 32 949 code regions of protein.In the meanwhile，bioinformatics analysis showed that 884 Unigenes were the transcript factor candidate of 55 families，and 2 261 Unigenes were R gene candidate of 18 families，total of 6 591 SSR loci were detected，of which 2 base repeat SSR accounted for about 70%.
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备注/Memo
第一作者简介：王云生（1975-），男，博士，副教授，现主要从事遗传育种与园艺学及基因组学等研究工作。E-mail：wys3269@126.com.责任作者：聂飞（1965-），男，硕士，研究员，现主要从事蓝莓栽培推广等研究工作。E-mail：1134477929@qq.com.基金项目：国家自然科学基金资助项目（31560091）；贵州省科技厅重点资助项目（黔教合KY字[2013]186）；贵州省科技成果重点推广计划资助项目（黔科合成字[2014]5007-1号）。