[1]马坤,杨建军,李璐,等.接种丛枝菌根真菌对干旱胁迫下木棉幼苗地上部水分供应的影响[J].北方园艺,2017,41(16):95-101.[doi:10.11937/bfyy.20165147]
　MA Kun,YANG Jianjun,LI Lu,et al.Inoculation of Arbuscula Mycorrhizal Fungi Improved Water Supply in Aboveground Part of Bombax ceiba Seedling Under Drought Stress[J].Northern Horticulture,2017,41(16):95-101.[doi:10.11937/bfyy.20165147]
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接种丛枝菌根真菌对干旱胁迫下木棉幼苗地上部水分供应的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年16 页码: 95-101 栏目: 出版日期: 2017-08-30
Title:
Inoculation of Arbuscula Mycorrhizal Fungi Improved Water Supply in Aboveground Part of Bombax ceiba Seedling Under Drought Stress
作者:
马坤1; 杨建军2; 李璐1; 马焕成1; 王彦淇1; 王颖1
（1.西南林业大学 国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224； 2.云南省林业调查规划院 大理分院，云南 大理 671000）
Author(s):
MA Kun1; YANG Jianjun2; LI Lu1; MA Huancheng1; WANG Yanqi1; WANG Ying1
(1.Key Laboratory of Biodiversity Conservation in Southwest China，State Forestry Administration，Southwest Forestry University，Kunming，Yunnan 650224；2.Dali Branch,Yunnan Institute of Forestry Inventory and Planning，Dali，Yunnan 671000)
关键词:
木棉; 丛枝菌根真菌(AMF); 干旱胁迫
Keywords:
Bombax ceiba L.; arbuscula mycorrhizal fungi; drought stress
DOI:
10.11937/bfyy.20165147
文献标志码:
A
摘要:
以接种和不接种丛枝菌根真菌木棉幼苗为试材，采用盆栽试验的方法，研究了不同干旱胁迫对不同处理下木棉幼苗的根茎叶水分特征及其生长和生理生化特征，以期探究干旱河谷中的木棉（Bombax ceiba L.）普遍存在与丛枝菌根真菌(AMF)的共生系统是否是它对干旱环境适应的策略之一。结果表明：对照条件下接菌株根系干质量是未接菌株的2.2倍，重度干旱胁迫下，接菌株的根系干质量是未接菌株的2倍，接菌有助于植株根系的生长，增加根系对水分的吸收效率。接菌植株在中度干旱胁迫下避免了植株的“茎干缩”，重度胁迫下减轻了“茎干缩”，且接菌株茎含水率是未接菌株的1.95倍，接菌提高了植株茎干的吸水保水能力。在重度干旱胁迫下，接菌加速了植株调节体内游离氨基酸和丙二醛（MDA）含量，较未接菌株提前了约18 d，且未接菌株MDA含量的最大值是接菌株的1.5倍；接菌株脯氨酸含量的最大值是未接菌株的1.4倍，接种显著增加了渗透物质含量并减少了细胞的伤害，提高了植物叶片的保水能力。可见，接种AMF可改善木棉幼苗干旱胁迫下根茎的水分供应和叶片水分维持能力，同时也增加渗透调节物质以降低水势，减少水分蒸散作用，启动调节系统提前清除细胞有害物质，减少对细胞膜的伤害，提高了木棉的抗旱性。
Abstract:
The plants of Bombax ceiba always have a symbiotic system with arbuscular mycorrhizal fungi (AMF) in the dry-hot valley.To study weather the symbiotic system one of the key strategies when plants adapting to the drought stress,a series of pot experiment was carried out to determine whether the AMF could affect the growth and the physiological process in B.ceiba under drought stress.The data on the characteristics of water content，growth and physiology，biochemistry of the root，stem and leaf was analyzed.It turned out that plants could benefit from the symbiotic system under drought stress.Firstly，the inoculation of AMF could improve the growth.Under no drought stress，the amount of the dry weight of roots with inoculation of AMF was 2.2 times of those without inoculation.Simultaneously，under severe drought condition，the amount of dry weight of roots with inoculation of AMF was 2 times of those without inoculation of AMF.It indicated that inoculation of AMF enhanced the yield of the root system and increased the efficiency of water absorption.Secondly，inoculation of AMF was helpful to avoid the stem shrinkage under drought stress.It reduced the loss of water contained in stem.Under the serve drought stress，the amount of water content in the inoculated plants was 1.95 times of those without inoculation.So，inoculation of AMF contributed to improving the capacity of water absorption and containing.Thirdly，inoculation of AMF accelerated the accumulation speed of the contents of amino acid and MDA.The peak of their contents appeared 18 days earlier in inoculated plants than those in non-inoculated plants under several stress.The maximum contents of MDA and proline in inoculated plants were 1.5 times and 1.4 times of those without inoculation under drought stress.It was obvious that AMF significantly increased the penetration levels and reduced cell damages，and improved the water retention capacity of plant leaves.Therefore，it could be suggested that inoculation of AMF could improve the drought resistance in B.ceiba by increasing the plant growth and water absorption and retaining and accelerating the osmotic regulation by accumulating the MDA and porline.

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备注/Memo

第一作者简介：马坤（1990-），男，硕士研究生，研究方向为生态公益林培育理论与技术。E-mail：makunweige@qq.com. 责任作者：李璐（1974-），女，博士，副研究员，研究方向为植物适应与进化。E-mail：lilusaraca@foxmail.com. 基金项目：国家自然科学基金资助项目（31260175，31560207）；云南省教育厅科学研究基金产业化培育资助项目（2016CYH14）；云南省“云岭教学名师”培育和云南省省级重点学科(林学)共同资助项目。收稿日期：2017-03-07

更新日期/Last Update: 2017-08-31