[1]袁恩平,屈用函,赵昶灵,等.低温胁迫下文山辣椒的抗氧化能力及其与花色苷含量的非相关性[J].北方园艺,2017,41(20):18-24.[doi:10.11937/bfyy.20164990]
　YUAN Enping,QU Yonghan,ZHAO Changling,et al.Antioxidant Capacity of Capsicum annuum Cultivars in Wenshan Prefecture Under Low Temperature Stress and Its None Correlation With Anthocyanin Contents[J].Northern Horticulture,2017,41(20):18-24.[doi:10.11937/bfyy.20164990]
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低温胁迫下文山辣椒的抗氧化能力及其与花色苷含量的非相关性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 18-24 栏目: 出版日期: 2017-10-30
Title:
Antioxidant Capacity of Capsicum annuum Cultivars in Wenshan Prefecture Under Low Temperature Stress and Its None Correlation With Anthocyanin Contents
作者:
袁恩平1; 屈用函1; 赵昶灵2; 赵水灵1; 张洪玲2; 张雪廷1
1.文山农业科学院，云南 文山 663000；2.云南农业大学 农学与生物技术学院，云南 昆明 650201
Author(s):
YUAN Enping1; QU Yonghan1; ZHAO Changling2; ZHAO Shuiling1; ZHANG Hongling2; ZHANG Xueting1; WANG Shaoxiang1; YANG Lingyu1; LI Yun1
1.Wenshan Academy of Agricultural Sciences,Wenshan,Yunnan 663000; 2.College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan 650201
关键词:
云南文山; 辣椒; 品种; 冰水模拟; 低温胁迫; 抗氧化能力; 花色苷含量
Keywords:
Wenshan in Yunnan Province;  Capsicum annuum;  cultivars;  simulated by icy water;  low temperature stress;  antioxidant capacity;  anthocyanin content
DOI:
10.11937/bfyy.20164990
文献标志码:
A
摘要:
以云南文山州主栽辣椒品种‘ZSZ75-1’‘ZSZ49-1-1’‘12WS-18-1’‘12ZH01’和‘ZS130’为试材，在冰水模拟低温胁迫下，用分光光度法研究了叶片的抗氧化酶比活力及总花色苷和丙二醛(MDA）含量，并用隶属函数法分析了叶片的综合抗氧化能力。结果表明：在低温胁迫下，5个辣椒品种叶片可溶性蛋白质含量特征为‘12WS-18-1’＞‘12ZH01’＞‘ZS130’＞‘ZSZ49-1-1’＞‘ZSZ75-1’，过氧化氢酶(CAT）比活力为‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘12WS-18-1’＞‘ZS130’，过氧化物酶(POD）比活力为‘ZSZ75-1’＞‘ZS130’＞‘ZSZ49-1-1’＞‘12WS-18-1’＞‘12ZH01’，超氧化物歧化酶(SOD）比活力为‘ZS130’＞‘12WS-18-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘ZSZ75-1’，MDA含量为‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘12WS-18-1’＞‘ZS130’，综合抗氧化能力为‘ZSZ49-1-1’＞‘ZS130’＞‘12WS-18-1’＞‘12ZH01’＞‘ZSZ75-1’，总花色苷含量为‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘ZS130’＞‘12WS-18-1’。此外，5个辣椒品种叶片总花色苷和MDA含量间均达差异极显著水平，而CAT、POD和SOD比活力间均未达差异显著水平。因此，低温胁迫下，文山辣椒叶片的抗氧化能力与其花色苷含量无关。该研究可为文山州辣椒品种耐低温性的评价和耐低温新品种的选育提供参考。
Abstract:
The specific activities of the antioxidases and total anthocyanin and MDA contents of the leaves of the five main Capsicum annuum cultivars including ‘ZSZ75-1’‘ZSZ49-1-1’‘12WS-18-1’‘12ZH01’ and ‘ZS130’ cultivated in Wenshan prefecture,Yunnan Province under the low temperature stress simulated by icy water were studied by using spectrophotometry,and the comprehensive antioxidant capacity of the leaves was evaluated by using subordinate function.The results showed that,under the low temperature stress,the soluble protein contents of the leaves of the five cultivars was as,‘12WS-18-1’＞‘12ZH01’＞‘ZS130’＞‘ZSZ49-1-1’＞‘ZSZ75-1’,CAT specific activities was as,‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘12WS-18-1’＞‘ZS130’,POD specific activities was as,‘ZSZ75-1’＞‘ZS130’＞‘ZSZ49-1-1’＞‘12WS-18-1’＞‘12ZH01’,SOD specific activities was as,‘ZS130’＞‘12WS-18-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘ZSZ75-1’,the MDA contents was as,‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘12WS-18-1’＞‘ZS130’,the comprehensive antioxidant ability was as,‘ZSZ49-1-1’＞‘ZS130’＞‘12WS-18-1’＞‘12ZH01’＞‘ZSZ75-1’,and the total anthocyanin contents was as,‘ZSZ75-1’＞‘ZSZ49-1-1’＞‘12ZH01’＞‘ZS130’＞‘12WS-18-1’.Furthermore,the differences among the total anthocyanin and MDA contents of the leaves of the five cultivars all reached the extremely significant levels,whereas the differences among the specific activities of CAT,POD and SOD did not reach the significant difference levels.Thus,under low temperature stress,the leaf antioxidant ability of the C.annuum cultivars cultivated in Wenshan prefecture was not related to the leaf anthocyanin contents.This study could provide a reference for the low temperature tolerance-evaluating and the selection of the new low temperature tolerant cultivars of C.annuum in Wenshan.

参考文献/References:

[1]CHALKER-SCOTT L.Environmental significance of anthocyanins in plant stress responses[J].Photochemistry and Photobiology,1999,70(1): 1-9.

[2]孙明霞,王宝增,范海,等.叶片中的花色素苷及其对植物适应环境的意义[J].植物生理学通讯,2003,39(6):688-694.
[3]GOULD K S.Nature′s Swiss army knife:The diverse protective roles of anthocyanins in leaves[J].Journal of Biomedicine and Biotechnology,2004(5):314-320.
[4]李颖畅,孟宪军,孙靖靖,等.蓝莓花色苷的降血脂和抗氧化作用[J].食品与发酵工业,2008,34(10):44-48.
[5]LI Y,MENG F L,ZHAO C L,et al.Responses of anthocyanin and osmolyte contents of the Capsicum annuum cultivars planted in Wenshan Prefecture of Yunnan Province to the drought stress simulated by PEG-6000[J].Agricultural Science & Technology,2016,17(6):1295-1300,1335.
[6]陈宗瑜.云南气候总论[M].北京:气象出版社,2001.
[7]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[8]谷会,弓德强,朱世江,等.冷激处理对辣椒冷害及抗氧化防御体系的影响[J].中国农业科学,2011,44(12):2523-2530.
[9]罗俊杰,欧巧明,叶春雷,等.重要胡麻栽培品种的抗旱性综合评价及指标筛选[J].作物学报,2014,40(7):1259-1273.
[10]NISHIYAMA Y,YAMAKAWA T.Effect of medium composition on the production of anthocyanins by hairy root cultures of Ipomoea batatas[J].Plant Biotechnology,2004,21(5):411-414.
[11]周琼琼,孙威江.茶树芽叶紫化的生理生化分析及其关键酶基因的表达[J].生物技术通报,2015,31(1): 86-91.
[12]杨莎,马艳青,朱晨曦,等.辣椒耐低温性鉴定技术标准[J].辣椒杂志,2014(2):11-13.
[13]肖强,郑海雷,陈瑶,等.盐度对互花米草生长及脯氨酸/可溶性糖和蛋白质含量的影响[J].生态学杂志,2005,24(4):373-376.
[14]马艳青,戴雄泽.低温胁迫对辣椒抗寒性相关生理指标的影响[J].湖南农业大学学报(自然科学版),2000,26(6):461-462.
[15]赵恒栋,李明,王怀栋,等.不同辣椒品种耐低温性综合评价[J].北方园艺,2015(23):22-25.
[16]王慧,周小梅.低温胁迫对辣椒相关生理生化指标的影响[J].山西农业科学,2015(2):152-154.
[17]GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48(12): 909-930.
[18]常彩涛.辣椒耐低温研究概述[J].辣椒杂志,2011(1):6-8,40.
[19]任旭琴,张林青,孙敏.辣椒叶片对低温的生理响应研究[J].安徽农业科学,2006,34(24):6439-6440.
[20]FAROOQ M,WAHID A,KOBAYASHI N,et al.Plant drought stress: Effects,mechanisms and management[J].Agronomy for Sustainable Development,2009,29(1):185-212.
[21]SARUYAMA H,TANIDA M.Effect of chilling on activated oxygen-scavenging enzymes in low temperature sensitive and tolerant cultivars of rice (Oryza sativa L.)[J].Plant Science,1995,109(2):105-113.
[22]王金亭.四种黑米花色苷抗氧化活性的构效关系[J].食品科技,2008,33(4):143-145.

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备注/Memo

第一作者简介：袁恩平(1985-），男，山东青州人，硕士，农艺师，现主要从事辣椒栽培与育种等研究工作。E-mail：407629929@qq.com.

责任作者：赵昶灵(1969-），男，四川都江堰人，博士，教授，博士生导师，现主要从事植物生理生化和分子生物学等研究工作。E-mail：zhaoplumblossom7@163.com.
基金项目：云南省对外科技合作计划资助项目(2015IC057）；云南省科技创新强省计划资助项目(2015AB011）；文山农业科学院资助项目(HZ2016-01）。
收稿日期：2017-06-27

更新日期/Last Update: 2017-10-31