ZHU Juanjuan,HE Huayu,LIU Wangsuo,et al.Nutrient Components and Active Substances of L.barbarum var.implicatum Leaves[J].Northern Horticulture,2016,40(23):145-149.[doi:10.11937/bfyy.201623034]
密枝枸杞叶营养成分和活性物质研究
- Title:
- Nutrient Components and Active Substances of L.barbarum var.implicatum Leaves
- Keywords:
- L.barbarum var.implicatum; ‘Ningqi No.5’; wolfberry leaves; nutrient compositions; active substances
- 文献标志码:
- A
- 摘要:
- 以密枝枸杞为试材,以“宁杞5号”枸杞为对照,采用分光光度法、Folin-Ciocalteu法、苯酚-硫酸比色法、四苯硼钠比色法、凯氏定氮法和2,6-二氯酚靛酚滴定法分别测定枸杞叶片中总黄酮、总多酚、粗多糖、蛋白质、抗坏血酸和钾元素含量,研究了密枝枸杞叶片营养品质价值。结果表明:密枝枸杞和“宁杞5号”叶中除抗坏血酸含量无显著性差异(P>0.05)外,其它各成分含量均存在显著性差异(P≤0.05)。密枝枸杞叶中总黄酮、总多酚、蛋白质含量显著高于“宁杞5号”枸杞叶(P≤0.05),而粗多糖、钾的含量极显著高于“宁杞5号”枸杞叶(P≤0.01)。与“宁杞5号”枸杞叶相比,密枝枸杞叶中总黄酮、总多酚、蛋白质、粗多糖和钾的含量分别高2.97%、5.93%、6.42%、8.31%和33.76%,但抗坏血酸含量低32.34%。
- Abstract:
- L.barbarum var. implicatum used as test material,‘Ningqi No.5’ as control.By using spectrophotometry,Folin-Ciocalteu method,phenol-sulfuric acid colorimetry,four benzene boron sodium colorimetry,kjeldahl determination and 2,6-dichloro phenol indophenol titration total flavonoids,total polyphenols,crude polysaccharide,proteins,ascorbic acid,and potassium (K) in leaves,were measured,in order to evaluate nutritional quality of L.barbarum var. implicatum.The results indicated that no significant difference (P>0.05) was found in ascorbic acid content between L.barbarum var.implicatum and ‘Ningqi No.5’.The total flavonoids,total polyphenols,protein contents in leaves of L.barbarum var. implicatum were significantly higher than those in leaves of ‘Ningqi No.5’ (P0.05).And crude polysaccharide and K content were significantly higher in L.barbarum var.implicatum leaves than in ‘Ningqi No.5’leaves (P0.01).Compared with ‘Ningqi No.5’ leaves,the total flavonoids,total polyphenols,protein,crude polysaccharide,K content in L.barbarum var.implicatum leaves were higher by 2.97%,5.93%,6.42%,8.31% and 33.76%,respectively,but ascorbic acid content was lower by 32.34%.
参考文献/References:
[1]陈天云,蒋旭亮,张志耘.宁夏枸杞属(茄科)一新种和一新变种[J].广西植物,2012,32(1):5-8. [2]YEH Y C,HAHM T S,SABLIOV C M,et al.Effects of Chinese wolfberry (Lycium chinese P.Mill) leaf hydro lysates on the growth of ped iococcusacidilactici[J].Bioresource Technology,2008,99:1383-1393. [3]马婷婷,张旭,饶建华.枸杞叶成分及药理作用研究进展[J].北方园艺,2011(13):194-196. [4]牛冬玲,马婷婷,张永梅,等.宁夏枸杞不同品种叶的总黄酮含量及清除自由基能力分析[J].广西植物,2013,33(4):556-559. [5]李南薇,钟兆敬.枸杞叶多酚的提取及抗氧化活性研究[J].食品工业,2013,34(2):63-65. [6]刘源才,孙细珍,许银,等.枸杞多糖组成及含量测定方法的改进[J].食品科学,2013,34(12):292-295. [7]孙存华.教学中采用四苯硼钠比色法测定钾含量的改进[J].植物生理学通讯,1998,3(32):74-75. [8]张静华,吴婉坡,张举,等.应用2,6-二氯酚靛酚法测定草莓还原型抗坏血酸[J].哈尔滨师范大学(自然科学学报),1999,5(3):96-101. [9]吴莉莉,韦若勋,杨庆文,等.枸杞属(茄科)新类群杂交起源初探[J].广西植物,2011,31(3):304-311. [10]王娅丽,刘思洋,邵千顺,等.宁杞9号枸杞叶芽营养成分分析[J].经济林研究,2015,33(4):106-110.
备注/Memo
第一作者简介:朱娟娟(1981-),女,博士,讲师,现主要从事植物营养生理等研究工作。E-mail:zjj0954@163.com. 责任作者:马海军(1974-),男,博士,副教授,现主要从事果树栽培与果酒酿造等研究工作。E-mail:mahaijun2007@126.com. 基金项目:宁夏自然科学基金资助项目(NZ12114);宁夏葡萄与葡萄酒创新中心资助项目(1507)。