JIANG Qianqian,ZHANG Baoren,WANG Chengjian,et al.Effect of Exogenous Hydrogen Sulfide on Chlorophyll Fluorescence Parameters and Antioxidant Activity of Cutting Dianthus caryophyllus L.[J].Northern Horticulture,2016,40(23):124-128.[doi:10.11937/bfyy.201623028]
外源硫化氢对康乃馨切花叶绿素荧光参数和抗氧化酶活性的影响
- Title:
- Effect of Exogenous Hydrogen Sulfide on Chlorophyll Fluorescence Parameters and Antioxidant Activity of Cutting Dianthus caryophyllus L.
- Keywords:
- hydrogen sulfide(H2S); Dianthus caryophyllus L.; chlorophyll fluorescence parameters; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以康乃馨（Dianthus caryophyllus L.）切花为试材，瓶插液中加入不同浓度的硫化氢(H2S)供体硫氢化钠（NaHS），以蒸馏水处理为对照，研究H2S对康乃馨叶片叶绿素荧光参数和抗氧化酶活性的影响，以期探讨外源硫化氢（H2S）对康乃馨切花保鲜的调控机理。结果表明：在康乃馨切花衰老过程中，叶片叶绿素含量、可溶性糖含量降低，暗下光系统Ⅱ最大光化学效率（Fv/Fm）、潜在活性（Fv/Fo）、光下实际光化学效率ΦPSⅡ降低。与对照相比，适宜浓度的NaHS溶液能延长切花寿命，增大花径，增加叶片可溶性糖、叶绿素含量，Fv/Fm、Fv/Fo、ΦPSⅡ明显升高。其中，以600 μmol?L-1NaHS溶液处理的效果最好。瓶插第4天600 μmol?L-1NaHS溶液处理的丙二醛（MDA）含量显著低于对照，而超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性显著高于对照。可见，外源H2S可增强康乃馨切花衰老时活性氧清除能力，减轻叶片光合结构的伤害，延长花期，提升观赏价值。
- Abstract:
- Cutting flowers of Dianthus caryophyllus L.were used as materials.The effect of sodium hydrosulfide (NaHS，the donor of H2S) on chlorophyll fluorescence parameters and antioxidant activity in Dianthus caryophyllus L.were investigated to study the regulation mechanism of exogenous hydrogen sulfide (H2S) on preservation of cutting Dianthus caryophyllus L.，with distilled water as the control.The results showed that chlorophyll content，soluble sugar content，maximum photochemical efficiency of PS II in darkness (Fv/Fm)，potential activity of PS II (Fv/Fo) and actual photochemical efficiency of PSll (ΦPSⅡ) decreased in the senescence process of cutting flowers of Dianthus caryophyllus L.Compared with control，the appropriate concentration of NaHS solution treatment led to extension of cut flower life，enlargement in flower diameter，increase in chlorophyll content,soluble sugar content，and Fv/Fm，Fv/Fo，ΦPSII values，of which 600 μmol?L-1NaHS showed the best effect.600 μmol?L-1NaHS caused significant decrease in MDA content but increase in SOD，POD，CAT activities on the fourth day in vase.Those results indicated that exogenous H2S could enhance the reactive oxygen species (ROS) scavenging activity and alleviate the injury of photosynthetic apparatus in flowers of Dianthus caryophyllus L.against senescence，so extend the flowering and enhance ornamental value.
参考文献/References:
[1]马丽.磷酸钠对康乃馨切花保鲜效果的影响[J].湖北农业科学，2015，54(11)：2698-2701. [2]张燕琴，李方，郭延平，等.叶片叶绿素荧光参数用作采后切花菊衰老指标[J].浙江大学学报，2005，31(6)：683-688. [3]张荣佳，任菲，白艳波，等.基于快速叶绿素荧光诱导动力学分析逆境对PSⅡ影响的研究进展[J].安徽农业科学，2012，40(7)：3858-3859. [4]郭鸿鸣，肖天宇，谢彦杰.气体信号分子硫化氢在植物中的生理功能及作用机制[J].中国生物化学与分子生物学报，2016，32(5)：488-495. [5]周超凡，吴帼秀，李婷，等.外源 H2S 对低温下日光温室黄瓜光合作用及抗氧化系统的影响[J].园艺学报，2016，43(3)：462-472. [6]ALI B，GILL R A，YANG S，et al.Hydrogen sulfide alleviates cadmium-induced morpho-physiological and ultrastructural changes in Brassica napus[J].Ecotoxicology and Environmental Safety，2014，110：97-207. [7]ZHANG L P，WANG H J，JIN Z P，et al.Hydrogen sulfide alleviates cadmium-induced cell death through restraining ROS accumulation in roots of Brassica rapa L.ssp.pekinensis[J].Oxidative Medicine and Cellular Longevity，2015(1):1-11. [8]CHEN J，WU F H，WANG W H，et al.Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis，photosynthetic enzyme expression，and thiol redox modification in Spinacia oleracea seedlings[J].J Exp Bot，2011，62：4481-4493. [9]ZHANG H，HU S L，ZHANG Z J，et al.Hydrogen sulfide acts as a regulator of flower senescence in plants[J].Postharvest Biology and Technology，2011，60(3)：251-257. [10]赵世杰，史国安，董新纯.植物生理学实验指导[M].北京：中国农业科学技术出版社，2002. [11]李合生，孙群，赵世杰，等.植物生理生化实验原理和技术[M].北京：高等教育出版社，2000. [12]孟丹，安敏敏，杨立明.硫化氢缓解铝胁迫对水稻幼苗叶片抗氧化系统的调控[J].淮阴师范学院学报（自然科学版），2015，14(1)：53-55. [13]侯智慧，车永梅，王兰香，等.H2S 位于 H2O2下游参与乙烯诱导拟南芥气孔关闭过程[J].植物生理学报，2012，48(12):1193-1199. [14]DEMMIG-ADAMS B，WILLIAM W.Xanthophyll cycle and light stress in nature：uniform response to excess direct sunlight among higher plant species[J].Planta,1996，198(3):460-470. [15]黄亚成，秦云霞.植物中活性氧的研究进展[J].中国农学通报，2012，28(36)：219-226. [16]KORNYEYEV D，LOGAN B A，PAYTON P，et al.Enhanced photochemical light utilization and decreased chilling-induced photoinhibition of photosystem II in cotton overexpressing genes encoding chloroplast-targeted antioxidant enzymes[J].Physiologia Plantarum,2001,113(3)：323-331.
相似文献/References:
[1]徐玲丽,张鸿翎,韩玲玲,等.鲜切花家用保鲜剂的配方研究[J].北方园艺,2012,36(19):165.
XU Ling-li,ZHANG Hong-ling,HAN Ling-ling,et al.Study on the Formula of the Preservation of Cut Flower Freshness[J].Northern Horticulture,2012,36(23):165.
[2]周燕,谢振财,樊磊.硫代硫酸银对康乃馨鲜切花保鲜效果的影响[J].北方园艺,2013,37(24):140.
ZHOU Yan,XIE Zhen-cai,FAN Lei.Effect of STS on Preservation of Cutting Dianthus caryophyllus[J].Northern Horticulture,2013,37(23):140.
[3]殷 福 姣.康乃馨扦插繁殖中对生根剂的优选[J].北方园艺,2008,32(03):0.[doi:10.11937/bfyy.200803081]
YIN Fu -Jiao.Choosing the Better Method of Using Hormone Deal with Carnation Cuttings in Carnation ’ sAsexual Propagation from Cutting[J].Northern Horticulture,2008,32(23):0.[doi:10.11937/bfyy.200803081]
[4]刘琦琦,徐娟,王黎明,等.外源H.2S对“尖脆”枣果实贮藏品质及抗氧化能力的影响[J].北方园艺,2018,42(22):135.[doi:10.11937/bfyy.20180139]
LIU Qiqi,XU Juan,WANG Liming,et al.Effect of Exogenous Hydrogen Sulfide on Storage Quality and Antioxidant Capacity of Postharvest ‘Jiancui’ Jujube Fruits[J].Northern Horticulture,2018,42(23):135.[doi:10.11937/bfyy.20180139]
[5]王爱国,姜倩倩,王淑子,等.外源硫化氢对温度胁迫下草莓开花进程和生理特性的影响[J].北方园艺,2022,(14):37.[doi:10.11937/bfyy.20220268]
WANG Aiguo,JIANG Qianqian,WANG Shuzi,et al.Effects of Exogenous Hydrogen Sulfide on the Flowering Process and Physiological Characteristics of Strawberry Under Temperature Stress[J].Northern Horticulture,2022,(23):37.[doi:10.11937/bfyy.20220268]
备注/Memo
第一作者简介：姜倩倩（1983-），女，博士，讲师，现主要从事果树逆境生理与分子生物学等研究工作。E-mail:jiangqq5238@163.com. 基金项目：国家自然科学基金资助项目（31301733）；潍坊市科技发展计划资助项目（20121303）；潍坊学院博士基金资助项目（2012BS18）。