CUI Ruihang,FAN Shaoran,JIAO Meiling,et al.Evaluation of Heat Resistance and Cold Resistance of Grandiflora Roses by Bioelectrical Impedance Technology[J].Northern Horticulture,2016,40(22):75-81.[doi:10.11937/bfyy.201622020]
生物电阻抗技术评价大花月季的耐热性及抗寒性
- Title:
- Evaluation of Heat Resistance and Cold Resistance of Grandiflora Roses by Bioelectrical Impedance Technology
- 文献标志码:
- A
- 摘要:
- 以2年生大花月季品种“黑魔术”“金奖章”“全美小姐”当年生枝条为试材，采用电阻抗图谱（EIS)法研究了茎在4—8月的耐热性与9月至翌年1月的抗寒性，并对高温、低温胁迫下电阻抗参数的变化进行了详细研究，以期确定各电阻抗参数对温度胁迫的响应，并得到不经温度处理估测半致死温度的最适参数及响应回归方程。结果表明：胞外电阻率re和弛豫时间τ 随高温、re随低温处理的变化曲线能用Logistic方程得到较好拟合结果；不经温度处理样本最适估测高温半致死温度的参数分别为“黑魔术”胞内电阻率ri（r=0.795)、“金奖章” ri（r=0.809)、“全美小姐”τ（r=-0.858)；最适估测低温半致死温度的参数分别为“黑魔术”弛豫时间发布系数ψ（r=0.967)、“金奖章”re（r=-0.873)、“全美小姐”ψ（r=0.922)。
- Abstract:
- The one-year-old shoots in two-year-old grandiflora roses ‘Magia Nera’ ‘Gold Medal’‘Maria Callas’ were used as materials.The heat resistance of stems from April to August and the cold resistance of stems from September to the next January were measured by the method of electrical impedance spectroscopy (EIS).And the changes of EIS parameters in high temperature and low temperature stresses were studied in detail.The aims of the present study were to determine the response of EIS parameters to temperature stress，and to obtain the best EIS parameters for estimating the semi-lethal temperatures without temperature test.The results showed that under high temperature stress test，the changes of extracellular resistance re and relaxation time τ could be well fitted by Logistic equation；and under low temperature stress test，re could be well fitted by Logistic equation.For the samples without temperature test，the best EIS parameters to estimate the semi-lethal of high temperature stress were intracellular resistance ri (r=0.795)，ri (r=0.809) and τ (r=-0.858) for ‘Magia Nera’‘Gold Medal’ and ‘Maria Callas’ respectively.The best EIS parameters to estimate the semi-lethal of low temperature stress were relaxation time distribution coefficient of ψ (r=0.967)，re (r=-0.873) and ψ (r=0.922) for ‘Magia Nera’‘Gold Medal’ and ‘Maria Callas’ respectively.
参考文献/References:
[1]王涛,田雪瑶,谢寅峰,等.植物耐热性研究进展[J].云南农业大学学报,2013,28(5):719-726. [2]姚斌.月季的抗性试验研究与选优[J].现代园艺,2013(6):16. [3]〖JP〗王玉全.丰花月季抗寒性研究[J].园艺与种苗,2012(7):32-34,46. [4]曾尚文.月季耐湿热性研究综述[J].南方林业科学，2015,43(4):25-28. [5]李素华,姬金凤.利用电导法鉴定几种野生花卉的抗寒性和耐热性[J].贵州农业科学,2012,40(11):182-184. [6]田洪润,高荣孚,王华芳.以电阻抗谱法研究植物细胞膜透性生物学[J].广东农业科学,2013(5):142-144. [7]金明丽,徐继忠,张钢.苹果砧木枝条电阻抗参数与其抗寒性的关系[J].园艺学报,2011,38(6):1045-1051. [8]ZHANG G,RYYPP A,REPO T.The electrical impedance spectroscopy of Scots pine needles during cold acclimation[J].Physiologia Plantarum,2000,115:385-392. [9]REPO T,ZHANG M I N,RYYPP A.Effects of freeze-thaw injury on parameters of distributed electrical circuits of stems and needles of Scots pine seedlings at different stages of acclimation[J].Journal of Experimental Botany,1994,45:823-833. [10]张军,赵慧娟,张钢,等.电阻抗图谱法在刺槐种植资源抗寒性测定中的应用[J].植物遗传资源学报,2009,10(3):419-425. [11]王刘环,弓瑞娟,张钢.抗寒锻炼期间丰花月季茎的电阻抗参数与抗寒性的关系[J].河北农业大学学报,2015,38(1):48-52. [12]MACDONALD J R,JOHNSON W B.Fundamentals of impedance spectroscopy[M]//BARSOUKOV E，MACDONALD J R，eds.Impedance Spectroscopy:Theory，Experiment and Applications.Hoboken，N J，USA:John Wiley & Sons,2005:1-26. [13]REPO T,LEINONEN M,PAAKKONEN T.Electrical impedance analysis of shoots of Scots pine：Intracellular resistance correlates with frost hardiness[C]//Proceedings of the Finnish-Japanese Workshop on Molecule and Physiological Aspects of Cold and Chilling Tolerance of Northern Crops.Jokioinen,Finland,1998:27-30. [14]刘易超,肖建忠,杨际双.高温胁迫对菊花电阻抗图谱参数的影响[J].植物科学学报,2012,30(2):198-203. [15]董军生,张军,周素锐,等.电阻抗法测定杨树无性系抗寒性[J].西北林学院学报,2014,29(3):105-108. [16]王爱芳,张钢,魏士春,等.不同发育时期樟子松的电阻抗参数与抗寒性的关系[J].生态学报,2008,28(11):5741-5749. [17]VINL A,REPO T.Impedance spectroscopy in frost hardiness evaluation of Rhododendron leaves[J].Annals of Botany,2000,86:799-805.
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备注/Memo
第一作者简介：崔睿航（1990-)，男，河北唐山人，硕士研究生，研究方向为观赏作物抗性生理生态。E-mail：946766243@qq.com.责任作者：张钢（1959-)，男，博士，教授，现主要从事观赏作物抗性生理生态的教学与科研等工作。E-mail：zhanggang1210@126.com.基金项目：国家自然科学基金资助项目（31272190)。