SHAO Shujun,WANG Dongyang,JI Nana,et al.Research Advance of Metabolomics and Its Applications on Fruits Postharvest[J].Northern Horticulture,2016,40(21):192-195.[doi:10.11937/bfyy.201621048]
代谢组学技术在果实采后生理研究中应用的研究进展
- Title:
- Research Advance of Metabolomics and Its Applications on Fruits Postharvest
- Keywords:
- metabolomics; fruits; postharvest physiology; review
- 文献标志码:
- A
- 摘要:
- 代谢组学是对生物体内代谢物组的含量或变化进行测定,进而研究生物体的整体功能状态的技术。在介绍代谢组学相关概念的基础上,重点介绍了代谢组学研究过程中的样品提取、分析技术和数据分析的常用方法,并对该技术在果实采后生理和病理方面的研究进展进行了综述,最后对该技术目前存在的问题和发展趋势进行了分析和展望,以期促进代谢组学技术在果实采后生理研究中的应用。
- Abstract:
- Metabolomics is the technique that focused on research the overall functional status of organism by measured the content or changes of the metabolites in the biological matrices.Metabolomics′ conception,extracting and analyzing technique,and usual data analytical methods were introduced.The applications of metabolomics on fruit postharvest physiology and pathology were also reviewed in this paper.At last,the flaws of metabolomics and its application prospect were proposed.It was expected that these would promote the applications of metabolomics on the fruits postharvest physiology research.
参考文献/References:
[1]唐惠儒,王玉兰.代谢组研究[J].生命科学,2007,19(3):272-280. [2]董登峰.代谢物组学方法及其在植物学研究中的应[J].广西植物,2007,27(5):765-769. [3]TWEEDALE H,NOTLEY MCROBB L,FERENCI T.Effect of slow growth on metabolism of Escherichia coli,as revealed by global metabolite pool (Metabolome) analysis[J].Bacteriol,1998,180(19):5109-5116. [4]谢跃生,潘桂湘,高秀梅,等.高效液相色谱技术在代谢组学研究中的应用[J].分析化学评述与进展,2006,34(11):1644-1648. [5]HANHINEVA K,AHARONI A.Metabolomics in fruit development[M].Springer Netherlands:Molecular Techniques in Crop Improvement,2010:675-693. [6]张敏,焦宏,郭捷.基于NMR代谢组学技术对山药化学成分的研究[J].食品工程,2014(4):19-22. [7]NICHOLSON J K,LINDON J C,HOLMES E.Metabonomics:understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189. [8]ZHANG X,QI L W,YI L,et al.Screening and identification of potential bioactive components in a combined prescription of Danggui buxue decoction using cell extraction coupled with high performance liquid chromatography[J].Biomed Chromatogr,2008,22(2):157-163. [9]NI Y,SU M,QIU Y,et al.Metabolic profiling using combined GC-MS and LC-MS provides a system sunderstanding of aristolochic acid induced nephrotoxicity in rat[J].FEBS Letters,2007,581(4):707-711. [10]CEVALLOS J M,REYES J I,ETXEBERRIA E,et al.Metabolomic analysis in food science:A review[J].Trends Food Science and Technology,2009,20(11-12):557-566. [11]毛煜,袁伯俊.代谢组学的研究现状与展望[J].中国新药杂志,2007,16(13):1005-1009. [12]冯利,曹芳瑞,刘新民,等.非靶向代谢组学生物样品采集和制备方法探讨[J].中南药学,2014,12(12):1217-122. [13]许国旺,路鑫,杨胜利.代谢组学研究进展[J].中国医学科学院学报,2007,29(6):701-711. [14]FERNIE A R,TRTHEWEY R N,KROTZKY A J,et al.Metabolite profiling:From diagnostics to systems biology[J].Nature Reviews Molecular Cell Biology,2004(5):1-7. [15]HALLKET J M,WATERMAN D,PRZYBOROWSKA A M,et al.Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS[J].Experimental Botany,2005,56:219-243. [16]SEGER C,STURM S.Analytical aspects of plant metabolite profiling platforms:Current standings and future aims[J].Proteome Research,2007(6):480-497. [17]DETTMER K,ARONOV P A,HAMMOCK B D.Mass-spectrometry based metabolomics[J].Mass Spectrometry Reviews,2007,26:51-78. [18]林艳萍,司端运,刘昌孝.液相色谱和质谱联用技术结合化学计量学应用于代谢组学的研究进展[J].分析化学,2007,35(10):1535-1540. [19]FARNETI B,BUSATTO N,KHOMENKO I,et al.Untargeted metabolomics investigation of volatile compounds involved in the development of apple superficial scald by PTR-ToF-MS[J].Metabolomics,2015(11):341-349. [20]JORDAN A,HAIDACHER S,HANEL G,et al.A high resolution and high sensitivity proton-transfer-reaction time-of-flight mass spectrometer (PTR-TOF-MS)[J].International Journal of Mass Spectrometry,2009,286:122-128. [21]CAPPELLIN L,KARL T,PROBST M,et al.On quantitative determination of volatile organic compound concentrations using proton transfer reaction time-of-flight mass spectrometry[J].Environmental Science and Technology,2012,46:2283-2290. [22]SOUKOULIS C,CAPPELLIN L,APREA E,et al.PTR-ToF-MS a novel rapid high sensitivity and non-invasive tool to monitor volatile compound release during fruit post-harvest storage:The case study of apple ripening[J].Food and Bioprocess Technology,2013(6):2831-2843. [23]HENG Y,SHU G L,XUE F B,et al.Research advances in plant metabolomics[J].Chinese Bulletin of Botany,2005,22(5):532-540. [24]张敏,焦宏,郭捷.基于NMR代谢组学技术对山药化学成分的研究[J].食品工程,2014(4):19-23. [25]向欢,高耀,彭国茳,等.葡萄籽提取物对健康人体尿液的代谢组学研究[J].食品工业科技,2015,36(12):344-348. [26]王穆,让蔚清,张琪,等.四物汤对环磷酰胺所致血虚证治疗后的代谢组学研究[J].中国中药杂志,2010,35(5):630-634. [27]ROYSTON G.Metabolomics of a Superorganism[J].Journal of Nutrition,2007,137(1):259-266. [28]许国庆,王斐.面向代谢组学数据的分类算法研究[D].沈阳:东北大学,2013. [29]SAITO K,DIXON R,WILLMITZER L,et al.Plant metabolomics[M].Berlin:Springer Science and Business Media,2006:141-154. [30]WANTE J,NORD S A,MORITA H,et al.From exogenous to endogenous:The inevitable imprint of mass spectrometry in metabolomics[J].Journal of Proteome Research,2007,6(2):459-468. [31]SANCHZ P E M,GARCIA L J,JOSE L M,et al.HRMAS-nuclear magnetic resonance spectroscopy characterization of tomato ‘flavor varieties’from Almería (Spain)[J].Food Research International,2011,44(10):3212-3221. [32]李晶,吴晓健,刘昌孝,等.代谢组学研究中数据处理新方法的应用[J].药学学报,2006,41(1):47-53. [33]CAMPBELL W M,STURIM D E,REYNOLDS D A.Support vector machines using GMM supervectors for speaker verification[J].Signal Processing Letters,IEEE,2006,13(5):308-311. [34]HOLMES E,NICHOLLS A W,LINDON J C,et a1.Chemometric models for toxicity classification based on NMR spectra of biofluids[J].Chemical Research in Toxicology,2000(13):471-478. [35]ERIKSSON L,JOHANSSON E,WOLD N K.Multi and megavariate data analysis:Principles and applications[M].Sweden:Umetrics Academy,2001:43-63. [36]BOULESTEIX A L,STRIMMER K.Partial least squares:A versatile tool for the analysis of high-dimensional genomic data[J].Briefings in Bioinformatics,2007,8(1):32-44. [37] 崔波,冉冉,许义,等.组学技术与果蔬采后质量和安全控制[J].食品安全质量检测学报,2013,4(6):1683-1691. [38]林燕.偏最小二乘变量筛选法及其应用研究[D].厦门:厦门大学,2007. [39]KHAKIMOV B,BAK S,ENGELSEN S B.High-throughput cereal metabolomics:Current analytical technologies,challenges and perspectives[J].Journal of Cereal Science,2013,12(3):1-26. [40]WISHART D S.Metabolomics:applications to food science and nutrition research[J].Trends Food Science and Technology,2008,19(9):482-493. [41]TIETEL Z,LEWINSOHN E,FALLIK E,et al.Elucidating the roles of ethanol fermentation metabolism in causing off-flavors in mandarins[J].Journal of Agricultural and Food Chemistry,2011,59(21):11779-11785. [42]SATORU T,TADASHI N,YOSUKE M,et al.A NMR-based,non-targeted multistep metabolic profiling revealed L-rhamnitol as a metabolite that characterised apples from different geographic origins[J].Food Chemistry,2015,174:163-172. [43]van DENDRIESSCHE T,SCHAFER H,VERLINDEN B E,et al.High-throughput NMR based metabolic profiling of Braeburn apple in relation to internal browning[J].Postharvest Biology and Technology,2013,80:18-24. [44]HATOUM D,ANNARATONE C,HERTOG M L A T M,et al.Targeted metabolomics study of‘Braeburn’apples during long-term storage[J].Postharvest Biology and Technology,2014,96:33-41. [45]LEE J,RUDELL D R,DAVIES P J,et al.Metabolic changes in 1-methylcyclopropene (1-MCP) treated‘Empire’apple fruit during storage[J].Metabolomics,2012,8(4):742-753. [46]MOZZI F,ORTIZ M E,BLECKWEDEL J,et al.Metabolomics as a tool for the comprehensive understanding of fermented and functional foods with lactic acid bacteria[J].Food Research International,2013,54(1):1152-1161.
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备注/Memo
第一作者简介:邵淑君(1990-),女,硕士研究生,研究方向为果蔬采后生理。E-mail:18766965255@163.com.责任作者:李富军(1977-),男,博士,教授,现主要从事果蔬采后生理等研究工作。E-mail:15898761364@163.com.基金项目:国家自然科学基金资助项目(31101587)。