XIONG Yunhai,WAN Lusheng.Sensitivity of Osmanthus fragrans Seeds to 60Co-γ Radiation and Its Medial Lethal Doses in Radiation Breeding[J].Northern Horticulture,2016,40(20):81-84.[doi:10.11937/bfyy.201620022]
桂花种子的60Co-γ 射线辐射敏感性及半致死剂量研究
- Title:
- Sensitivity of Osmanthus fragrans Seeds to 60Co-γ Radiation and Its Medial Lethal Doses in Radiation Breeding
- 文献标志码:
- A
- 摘要:
- 以金桂、银桂种子为试材，采用不同剂量60Co-γ 射线辐射种子，观察辐射对桂花种子发芽率及胚根生长的影响，以种子50%发芽率为基准，通过直线回归方程计算半致死剂量，以研究桂花辐射诱变育种，探讨桂花种子辐射剂量的问题，选育具有较高观赏价值的桂花优良新品种。结果表明：60Co-γ 射线辐射可引起桂花种子胚根缩短、发芽率降低；金桂与银桂种子的辐射半致死剂量分别为123.807、118.381 Gy，最大辐照量为250 Gy。辐射剂量与金桂和银桂种子的发芽率间呈高度负相关，相关系数分别为r=-0.910、r=-0.934。金桂种子辐射敏感性高于银桂种子。
- Abstract:
- In order to study the radiative mutation breeding of Osmanthus fragrans，explore the radiation dosages of Osmanthus fragrans and select new variety of Osmanthus fragrans having high ornamental value，the Osmanthus fragrans seeds from two different varieties(var.thunbergii Makino and var.latifolius Makino) were treated with different dosages of 60Co-γ ray (0,50,100，150，200，250 and 300 Gy)，and the seed germination rate and radicle growth were observed.Based on the germination rate 50%，LD50 doses of 60Co-γ radiation for the seeds were calculated using linear regression equation.The results showed that 60Co-γ radiation caused the seeds radicle length shortened，lower germination rate.LD50 doses of 60Co-γ radiation for the seeds of var.thunbergii Makino and var. latifolius Makino were 123.807 Gy and 118.381 Gy respectively.Their maximum radiation dose was 250 Gy.The seeds germination rate of Osmanthus fragrans had a high negative correlation with radiation dose，the correlation coefficient was r=-0.910，r=-0.934,respectively.The radiation sensitivity of var.thunbergii Makino seeds was higher than var.latifolius Makino seeds.
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备注/Memo
第一作者简介：熊运海（1964-），男，本科，教授，现主要从事园林植物资源开发与利用等研究工作。E-mail:xiongyunhai621@163.com.基金项目：重庆市教委科学技术研究资助项目（KJ131217）。