XU Meng,GUO Shaoxia,SUN Li,et al.Influence Factors on Induction，Proliferation and Differentiation of Protocorm of Cymbidium hybridum[J].Northern Horticulture,2016,40(16):108-110.[doi:10.11937/bfyy.201616028]
大花蕙兰原球茎诱导、增殖与分化影响因素研究
- Title:
- Influence Factors on Induction，Proliferation and Differentiation of Protocorm of Cymbidium hybridum
- Keywords:
- Cymbidium hybridum; protocorm; induction; proliferation; differentiation
- 文献标志码:
- A
- 摘要:
- 以大花蕙兰试管组织为试材，MS为基本培养基，研究了不同添加物6-BA、NAA、IBA、活性炭，对大花蕙兰原球茎诱导、增殖和分化的影响。结果表明：适宜原球茎诱导、增殖的最佳培养基为MS+1.00 mg?L-1 6-BA+0.1 mg?L-1 NAA，适宜原球茎分化的最佳培养基为MS+1.0 mg?L-1 6-BA+0.2 mg?L-1 NAA，在培养基中添加0.3%活性炭有利于原球茎的增殖和分化。
- Abstract:
- Taking the protocorm of Cymbidium hybridum as material，MS as basic meidium，the effect of different concentrations of 6-BA，NAA，IBA and activated carbon on protocorm induction，proliferation and differentiation were researched.The results showed that the best concentration for induction and propagation of protocorm was MS+1.00 mg?L-1 6-BA+0.1 mg?L-1 NAA，the best concentration for differentiation of protocorm was MS+1.0 mg?L-1 6-BA+0.2 mg?L-1 NAA，and the MS medium with 0.3% activated carbon was suitable for propagation and differentiation of protocorm.
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备注/Memo
第一作者简介：徐萌（1980-），女，硕士，实验师，研究方向为园林植物育种与栽培。E-mail：baiguo69@163.com.基金项目：青岛市建委资助项目（6602414061）。