ZHANG Yanju,LIU Qi,LIU Dong,et al.Virulence Differentiation and AFLP Analysis of Corynespora cassiicola[J].Northern Horticulture,2016,40(16):92-98.[doi:10.11937/bfyy.201616025]
黄瓜棒孢叶斑病菌的毒力分化及AFLP分析
- Title:
- Virulence Differentiation and AFLP Analysis of Corynespora cassiicola
- 文献标志码:
- A
- 摘要:
- 以6个黄瓜品种（系）为试材，以来自山东、辽宁、黑龙江的200个黄瓜棒孢叶斑病菌为供试菌株，采用苗期接种鉴定及AFLP分子标记技术，研究了黄瓜棒孢叶斑病菌的毒力分化。结果表明:黄瓜棒孢叶斑病菌存在生理小种分化现象，并将200个菌株分为4个生理小种，初步筛选出D0330、D1306、D0649和D2025作为研究黄瓜棒孢叶斑病菌生理小种分化的鉴别寄主备选材料；AFLPs与菌株的地理来源无关，与毒力存在相关性。
- Abstract:
- Taking six cucumber cultivars as materials and 200 Corynespora cassiicola strains isolated from Shandong，Liaoning and Heilongjiang Province as test strains，the virulence differentiation of C.cassiicola was tested by using the method of seeding artificial inoculation and AFLP.The results showed that D0330，D1306，D0649 and D2050 could be used as differential hosts setting for C.cassiicola and the 200 strains could be divided into 4 races by the differential hosts.Genetic diversity analysis for the 200 strains was carried out using AFLP markers which suggested that AFLPs were unrelated with the geographical origin but with the virulence.
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备注/Memo
第一作者简介：张艳菊（1968-），女，博士，教授，博士生导师，研究方向为植物病原生物学及植物病害综合防治。E-mail:zhangyanju1968@163.com.基金项目:黑龙江省自然科学基金资助项目（C201428)。