GONG Guanglu,GUI Yang,LU Yingying,et al.Optimization of Ultrasonic Detection Process of Dictyophora rubrovolvata Mycelium Total Polysaccharose by Response Surface Analysis[J].Northern Horticulture,2016,40(15):135-141.[doi:10.11937/bfyy.201615033]
响应面分析法优化红托竹荪菌丝总糖超声检测工艺
- Title:
- Optimization of Ultrasonic Detection Process of Dictyophora rubrovolvata Mycelium Total Polysaccharose by Response Surface Analysis
- 文献标志码:
- A
- 摘要:
- 以红托竹荪(Dictyophora rubrovolvata)为试材,采用木屑培养的方法,以木屑菌丝总糖含量为指标,研究了液料比、振幅、超声总时间、超声波开启时间4个因素对红托竹荪木屑菌丝总糖含量的影响,并通过响应面分析法获得红托竹荪木屑菌丝总糖检测最佳工艺。结果表明:在液料比11∶1 mL?g-1、振幅41%、超声总时间22 min、超声波开启时间2.7 s时,红托竹荪菌丝总糖的得率达1.18%。
- Abstract:
- Dictyophora rubrovolvata was used as test material,by sawdust culture,Mycelium polysaccharose as test index,the effects of liquid-material ratio,amplitude,processing time and ultrasonic duration of ultrasonic on D.rubrovolvata sawdust mycelium polysaccharose detection rate were investigated.Based on single factor experiments,response surface analysis was used to optimize the process parameters.The results showed that under the conditions of liquid-material ratio of 11∶1 mL?g-1,amplitude of 41%,processing time of 22 minutes and ultrasonic duration of 2.7 seconds,the D.rubrovolvata sawdust mycelium polysaccharose detection rate was 1.18%.
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备注/Memo
第一作者简介:龚光禄(1985-),男,硕士,研究实习员,研究方向为食药用菌。E-mail:gongguanglu0923@163.com.责任作者:朱国胜(1971-),男,博士,研究员,研究方向为药用植物伴生菌及食药用真菌研究。E-mail:zgsah@163.com.基金项目:贵州省农业攻关资助项目(黔科合NY[2014]3037号);贵州省科研机构服务企业行动计划资助项目(黔科合服企[2014]4006号);贵阳市与贵州省农业科学院农业科技合作资助项目(院地农科合字[2014]3号)。