[1]陈静芳,李荣华,王直亮,等.芸薹属SSR标记在菜薹中的通用性及其应用[J].北方园艺,2016,40(15):86-91.[doi:10.11937/bfyy.201615022]
　CHEN Jingfang,LI Ronghua,WANG Zhiliang,et al.Transferability and Application of Brassica SSR Markers in Flowering Chinese Cabbage[J].Northern Horticulture,2016,40(15):86-91.[doi:10.11937/bfyy.201615022]
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芸薹属SSR标记在菜薹中的通用性及其应用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年15 页码: 86-91 栏目: 出版日期: 2016-08-15
Title:
Transferability and Application of Brassica SSR Markers in Flowering Chinese Cabbage
作者:
陈静芳1; 李荣华1; 王直亮1; 夏岩石1; 郭培国1; Kadambot SIDDIQUE2
(1.广州大学 生命科学学院,作物抗逆国际合作研究中心，广东 广州 510006；2.Institute of Agriculture，University of Western Australia，WA 6009，Australia)
Author(s):
CHEN Jingfang1; LI Ronghua1; WANG Zhiliang1; XIA Yanshi1; GUO Peiguo1; Kadambot SIDDIQUE2
（1.International Crop Research Center for Stress Resistance/College of Life Sciences，Guangzhou University,Guangzhou，Guangdong 510006；2.Institute of Agriculture，University of Western Australia，WA 6009，Australia）
关键词:
芸薹属; SSR; 通用性; 菜薹; 遗传连锁图
Keywords:
Brassica; SSR; transferability; flowering Chinese cabbage; genetic linkage group
DOI:
10.11937/bfyy.201615022
文献标志码:
A
摘要:
以4份遗传背景差异较大的菜薹种质为试材，检测133对芸薹属SSR引物在菜薹中的PCR扩增效果，以期发现适用于菜薹的SSR标记。结果表明：有50对引物在4份菜薹种质中表现出较好的通用性，其扩增产物多在100~350 bp，多态性数目在1~4，共91个多态性位点。在此基础上，利用在“四九-19号菜心”和“3T6”中具有多态性的40对SSR引物，对130份以“四九-19号菜心”和“3T6”为亲本建立的F6重组自交系群体进行基因分型，构建了一个具有10个遗传群、总长度为641.39 cM的菜薹遗传连锁图。研究结果可为今后菜薹遗传多样性、基因定位和分子标记辅助育种提供理论参考。
Abstract:
In order to develop SSR markers suitable for flowering Chinese cabbage，four flowering Chinese cabbage genotypes different in genetic background were selected to detect the amplification effects of 133 Brassica SSR primers.The results showed that 50 of 133 Brassica SSR primer combinations were confirmed to have the property of transferability in flowering Chinese cabbage.The amplicons of these SSR mainly ranged from 100 to 350 bp.91 polymorphic loci among 4 genotypes were detected with 1 to 4 polymorphic locus/loci per SSR primer pair.The population containing 130 recombinant inbred lines from the cross of ‘Shijiu-19 Caixin’ and ‘3T6’ was genotyped by using the SSR primer combinations which producing polymorphic loci between parents，and a genetic linkage map containing 10 linkage groups was established with a length of 641.39 cM.These results could be beneficial for the genetic diversity analysis，QTL mapping and marker-assisted selection in breeding program of flowering Chinese cabbage.

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备注/Memo

第一作者简介：陈静芳（1990-），女，硕士研究生，研究方向为菜薹分子生物学。E-mail：jingfang.chen@qq.com.责任作者：郭培国（1963-），男，博士，教授，现主要从事作物逆境生物学和分子育种的教学与科研工作。E-mail：guopg@gzhu.edu.cn.基金项目：广州市属高校科技计划资助项目（1201420621）；广州市科技计划资助项目（2014J4100123）；广东省自然科学基金资助项目（2015A030310136，2015A030313500）；广东省普通高校国际暨港澳台合作创新平台及国际合作重大资助项目（2015KGJHZ015）；国家自然科学基金资助项目（30871526）。

更新日期/Last Update: 2016-08-29