WANG Han,WANG Rangjun,ZHAO Shuling,et al.Antibacterial Activity Evaluation of Chlorothalonil in vitro Against Pantoea agglomerans on Walnut[J].Northern Horticulture,2016,40(14):119-121.[doi:10.11937/bfyy.201614029]
农药百菌清对核桃黑斑病伴生病原菌成团泛菌的抑菌活性研究
- Title:
- Antibacterial Activity Evaluation of Chlorothalonil in vitro Against Pantoea agglomerans on Walnut
- Keywords:
- walnut; walnut blight; Pantoea agglomerans; bacteriostasis
- 文献标志码:
- A
- 摘要:
- 以核桃黑斑病伴生病原菌成团泛菌(Pantoea agglomerans)为供试菌,采用抑菌圈法和平板菌落计数法,研究了农药百菌清对核桃黑斑病伴生病原菌成团泛菌(Pantoea agglomerans)的抑菌作用。结果表明:农药百菌清对该病原菌具有较好的抑菌作用,50.00 mg?mL-1的农药百菌清处理96 h后,抑菌圈的平均直径为1.80 cm,其抑菌率达到93.9%,且具有时间与剂量效应。2种试验方法的抑菌趋势是一致的,平板菌落计数法测定的结果变化趋势较抑菌圈法明显。
- Abstract:
- Taking Pantoea agglomerans as test bacteria,the method of bacteriostatic circle and plate culture count were used to study chlorothalonil control effect on bacteriostasis of associated pathogen (Pantoea agglomerans).The results showed that chlorothalonil had good effect on bacteriostasis.Treatment with the concentration of 50.00 mg?mL-1 chlorothalonil for 96 hours,antibacterial rate reached to 93.9%,in a dose and time dependent manner.The trends of antibacterial by two test methods were the same,but the results of plate culture count were more obvious than those of method of bacteriostatic circle.
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备注/Memo
第一作者简介:王瀚(1975-),男,博士,教授,研究方向为微生物学。E-mail:wanghzhangy@126.com.基金项目:2015年甘肃省委组织部陇原青年创新人才扶持计划资助项目(2015-13);甘肃省教育厅高等学校科研资助项目(2015B-147);陇南师专2014年校级重点科研资助项目(2014LSZK01003)。