HU Yuefang.Determination of Three Phytosterols in Yam by Capillary Electrophoresis With Electrochemical Detection[J].Northern Horticulture,2016,40(12):162-165.[doi:10.11937/bfyy.201612040]
毛细管电泳-电化学检测法测定淮山中三种植物甾醇含量
- Title:
- Determination of Three Phytosterols in Yam by Capillary Electrophoresis With Electrochemical Detection
- 关键词:
- 毛细管电泳-电化学检测; 淮山; 麦角甾醇; 胆甾醇; β-谷甾醇
- Keywords:
- capillary electrophoresis-electrochemical detection; yam; ergosterol; cholesterol; β-sitosterol
- 文献标志码:
- A
- 摘要:
- 以淮山为试材，采用毛细管电泳-电化学检测（CE-ED)方法测定淮山中3种植物甾醇（麦角甾醇、胆甾醇、β-谷甾醇）的含量，考察了检测电位、运行缓冲液浓度和pH、分离电压及进样时间等对检测效果的影响。结果表明：在优化的条件下，对0.5 mg?L-1麦角甾醇、胆甾醇、β-谷甾醇混合标准溶液连续6次进行测定在10 min内实现了分离，线性范围分别为0.1~1 500、0.1~1 000、0.1~1 200 μg?L-1,检出限分别为0.03、0.04、0.03 μg?L-1，峰电流的相对标准偏差（RSD）分别为1.5%、1.7%、1.6%，迁移时间RSD分别为0.6%、0.7%、0.6%。该方法已用于淮山样品中胆甾醇、麦角甾醇和β-谷甾醇的测定，加标回收率在97.6%~100.3%，相对标准偏差（RSD）≤2.3%。
- Abstract:
- Taking yam as test material,a method of capillary electrophoresis coupled with electrochemical detection(CE-ED)was adopted for the determination of three phytosterol content (ergosterol,cholesterol,β-sitosterol)in yam.The effects of detection potential,concentration and pH of running buffer,separation voltage and injection time on the detection were investigated.The results showed that under the optimum conditions,a good baseline separation and highly sensitive detection were achieved in 10 minutes good linear relationship between peak currents and ergosterol ,cholesterol and β-sitosterol concentration ranging from 0.1-1 500 μg?L-1,0.1-1 000 μg?L-1,0.1-1 200 μg?L-1,with the detection limits(S/N=3)of 0.03 μg?L-1,0.04 μg?L-1,0.03 μg?L-1,respectively,the relative standard deviations(RSD)of peak currents were 1.5%,1.7%,1.6%,RSD of the migration time were 0.6%,0.7%,0.6%.The method was successfully applied to the assay of cholesterol,ergosterol and β-sitosterol in samples of yam.The spiked recoveries were in the range of 97.6%-100.3%,with relative standard deviations less than 2.3%．
参考文献/References:
[1]胡月芳.毛细管电泳-电化学检测法测定淮山中5种活性成分[J].分析科学学报,2014,30(4):533-536. [2]杨丰滇.传统山药和无硫山药β-谷甾醇、腺苷、亚油酸含量研究[D].郑州:河南中医学院,2014. [3]牛建平,孙瑞霞,孙剑辉.气相色谱-质谱法分析怀山药中的有机成分[J].河南师范大学学报(自然科学版),2007,5(2):122-125. [4]宋明杰,包海鹰,图力古尔,等.椭圆嗜蓝孢孔菌中四种甾类化合物的抗肿瘤活性及构效关系分析[J].菌物学报,2015,34(2):293-300. [5]孙变娜,沈和定,吴洪喜,等.14种石磺科贝类的胆甾醇含量测定[J].海洋科学,2015,39(1):24-28. [6]邱丰艳,丁力,曹红云.高效液相色谱法测定油脂中β-谷甾醇的含量[J].中国油脂,2014,39(7):91-95. [7]李思明,冯怡,曾星.HPLC-APCI-MS/MS法同时测定猪苓颗粒中麦角甾酮与麦角甾醇的含量[J].药物分析杂志,2014,34(4):649-653. [8]张峻松,徐如彦,薄云川,等.毛细管气相色谱法测定烟草中的甾醇类化合物[J].烟草科技,2007(8):27-31. [9]邱玉亮.高效毛细管电泳(HPCE)对优质小麦HMWGS的分离鉴定[J].麦类作物学报,2015,35(5):1-7. [10]毛师师,钦晓峰,周杏琴.毛细管电泳分析生物样本中儿茶酚氧甲基转移酶COMT活性[J].分析试验室,2015,34(8):874-877. [11]胡月芳,李建平,刘蓉,等.毛细管电泳-电致化学发光联用技术应用进展[J].理化检验-化学分册,2011,47(5):618-622. [12]DENG G H,CHEN S Y,WANG H,et al.Determination of active ingredients of Phyllanthus urinaria by capillary electrophoresis with amperometric detection[J].J Liq Chromatogr Related Technol,2012,35(17):2370. [13]彭友元.毛细管电泳电化学检测法测定蜂胶中的黄酮和酚酸[J].分析试验室,2011,30(3):54-57. [14]孔令瑶,汪云,曹玉华.毛细管电泳-电化学法对发芽黑米胚芽中C-氨基丁酸含量的检测[J].分析测试学报,2008,27(5):527-530. [15]邓光辉,王士伟,王辉,等.毛细管电泳安培法测定田基黄中的芦丁与槲皮素[J].分析试验室,2014,33(4):424-427.
相似文献/References:
[1]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(12):135.
[2]伍壮生,王 敏,吴月燕.海南省栽培的淮山种质资源聚类分析[J].北方园艺,2014,38(03):14.
WU Zhuang-sheng,WANG Min,WU Yue-yan.Cluster Analysis of Cutivated Chinese Yam Germplasm Resources in Hainan Province[J].Northern Horticulture,2014,38(12):14.
[3]唐秀梅,吴昌湛,唐荣华,等.淮山套种花生高效栽培技术[J].北方园艺,2015,39(13):68.[doi:10.11937/bfyy.201513021]
备注/Memo
作者简介：胡月芳（1977-），女，硕士，副教授，研究方向为电化学分析。E-mail:huyuefang@126.com.基金项目：广西高校科学技术研究资助项目(2013YB238)；贺州市科学研究与技术开发计划资助项目(贺科转1408035)。