GU Ruizhi,WU Yanyou,TENG Boqun,et al.Effect of Artificial Seed Coat Containing Nano TiO2 on Germination[J].Northern Horticulture,2016,40(12):20-24.[doi:10.11937/bfyy.201612005]
添加纳米TiO2制作的人工种皮对种子萌发的影响
- Title:
- Effect of Artificial Seed Coat Containing Nano TiO2 on Germination
- 文献标志码:
- A
- 摘要:
- 以诸葛菜组织培养中产生的不定芽为繁殖体,海藻酸钠为包埋基质,添加纳米TiO2制作人工种皮,观察添加不同浓度的纳米TiO2对诸葛菜人工种子萌发和抗菌能力的影响,将纳米TiO2与NAA以及多菌灵的作用进行对比并研究共同作用。结果表明:纳米TiO2具有与NAA相似的生长素作用,并且显著提高萌发生根速度,二者具有协同作用;无菌条件下0.1 mg?L-1 NAA+30 g?L-1 TiO2+1/2 MS+3%蔗糖的组合使诸葛菜人工种子萌发率达到100%,生根率达到80%,为最佳组合;有菌条件下添加30 g?L-1纳米TiO2试验组15 d污染率仅为5.6%,萌发率达83.33%,为最佳组合。纳米TiO2和多菌灵混合作用反而使萌发率和抗菌能力大幅降低。
- Abstract:
- The Orychophragmus violaceus adventitious buds produced in tissue culture were used as propagules,the sodium alginate added nano TiO2 was made into artificial seed coat.The effect of different concentration of nano TiO2 on artificial seeds germination and antibacterial ability was observed,the differences between nano TiO2 with NAA or carbendazim and the interactions of them were studied.The results showed that nano TiO2 had the similar auxin action with NAA,and significantly increased the germinaton and rooting rate,and the two had a synergistic effect.Combination 0.1 mg?L-1 NAA+30 g?L-1 TiO2+1/2MS+3% sucrose under sterile conditions made Orychophragmus violaceus artificial seeds’ germination rate reached 100% and rooting rate reached 80%,was the best combination;the experimental group added 30 g?L-1 nano TiO2 under natural conditions made contamination rate reached 5.6%,germination rate reached 83.33%,was the best combination.The experimental group containing both nano TiO2 and carbendazim significantly reduced the germination rate and antibacterial ability.
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备注/Memo
第一作者简介:谷睿智(1989-),男,硕士研究生,研究方向为植物组织培养。E-mail:532891474@qq.com.责任作者:吴沿友(1966-),男,博士,研究员,博士生导师,现主要从事生物技术与生态学等研究工作。E-mail:yanyouwu@ujs.edu.cn.基金项目:江苏省高校优势学科资助项目(苏政办[2014]37号)。