YANG Guohui,LYU Bingyu,HAN Deguo,et al.Change of Cell Wall Components and Related Enzyme Activities During the Development Process of Raspberry Fruit[J].Northern Horticulture,2016,40(11):27-30.[doi:10.11937/bfyy.201611007]
树莓果实发育过程中细胞壁成分及相关酶活性变化
- Title:
- Change of Cell Wall Components and Related Enzyme Activities During the Development Process of Raspberry Fruit
- Keywords:
- raspberry; fruit development; cell wall composition; enzyme
- 文献标志码:
- A
- 摘要:
- 以秋果型树莓“哈瑞太兹”和夏果型树莓“菲尔杜德”2种不同类型的树莓为试材，研究了绿果膨大期（花后7 d）、转白期（花后14 d）、着色期（花后21 d）、可采成熟期（花后24 d）和过熟期（花后27 d）树莓果实原果胶、果胶、纤维素含量及相关酶活性的变化情况。结果表明：随树莓果实的成熟软化，原果胶含量在着色期前表现为上升，之后下降；可溶性果胶呈现逐渐上升趋势，而纤维素含量则表现为降低趋势；多聚半乳糖醛酸酶（PG）、果胶甲酯酶（PME）和β-半乳糖苷酶（β-Gal）和纤维素酶在可采成熟期前均表现为上升趋势，尤其PG从着色期到可采成熟期上升迅速，而从可采成熟期到过熟期，只有PG酶表现为下降，其它3种酶仍表现为上升趋势。
- Abstract:
- ‘Henritage’of autumn type and‘Fertod Zamatos’of summer fruited type were used as experimental materials in this study.The raspberry fruits were collected on 7 days after flowering(the green fruiting enlargement period)，14 days(turning white period)，21 days(coloring period)，24 days(mature period) and 27 days(overmature period)，respectively.The contents of original pectin，pectin，cellulose and related enzyme activities of raspberry fruits were measured.The results showed that during the maturity and softening process of fruit，the protopectin content rose before coloring period，then declined.Soluble pectin content in fruit was gradually increased，but the cellulose content had a decreasing trend.The enzyme activities of polygalacturonase，pectin methylesterase，cellulase and β-galactosidase had the rising trend before mature period，especially the polygalacturonase activity increased rapidly from coloring period to maturity.From mature period to overmature，only the polygalacturonase activity declined，by contrast，other three enzymes activity still showed the rising trend.
参考文献/References:
[1]宣景宏，孟宪军，刘春菊，等.树莓的主要功效成分及开发利用前景[J].中国果业信息，2007,24(1)：2628. [2]鄂利东.树莓生产问题与发展对策[J].新农业,2011(2):26-27. [3]钱春，张兴国，梁国鲁.草莓果实成熟软化相关基因研究进展[J].中国蔬菜,2009(22)：6-12. [4]金昌海，索标，阚娟,等.桃果实成熟软化过程中细胞壁多糖降解特性的研究[J].扬州大学学报,2006,27(3):70-75. [5]张鹏龙，陈复生，杨宏顺,等.果实成熟软化过程中细胞壁降解研究进展[J].食品科技,2010,35(11):62-66. [6]刘美艳.早熟苹果果实软化分子机理的初步研究[D].泰安：山东农业大学，2012. [7]阚娟.不同溶质型桃果实成熟软化机理研究[D].扬州：扬州大学，2011. [8]佟兆国，王飞，高志红,等.果胶降解相关酶与果实成熟软化[J].果树学报,2011,28(2)：305-312. [9]PRIYA-SETHU K M.Post-harvest biochemical changes associated with the softening phenomenon in Capsicum annuum fruits[J].Phytochemistry，1996，43(4)：961-966. [10]阚娟，金昌海，汪志君,等.苹果β-半乳糖苷酶及多聚半乳糖醛酸酶对桃果实成熟软化的影响[J].扬州大学学报（农业与生命科学版）,2006,27(3):76-80. [11]YANG Z F，ZHENG Y H，CAO S F，et al.Effects of storage temperature on textural properties of Chinese bayberry fruit[J].Journal of Texture Studies,2007，38：166-177. [12]李萍，廖康，赵世荣,等.杏果实发育成熟过程中细胞壁组分和水解酶活性的变化[J].新疆农业大学学报,2013,36(4):298-303. [13]刘剑锋，李国怀，彭抒昂,等.秋子梨的果皮结构与果实的耐贮性[J].园艺学报,2007,34(4):1007-1010. [14]杨国慧，王冰，谢娟,等.树莓果实显微及超显微结构观察[J].湖北农业科学,2015,54(8):1919-1922. [15] CALLAHAN A M，SCORZA R，BASSETT C，et al.Deletions in an endopolygalacturonase gene cluster correlate with non-melting flesh texture in peach[J].Functional Plant Biology，2004，31(2)：159-168.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]宋健坤,宗 宇,王 然.长把梨果实早期发育动态研究[J].北方园艺,2013,37(23):1.
SONG Jian-kun,ZONG Yu,WANG Ran.Study on Fruit Development Dynamic of Long-stalk Pear During the Early Stage[J].Northern Horticulture,2013,37(11):1.
[3]王 娜,卢宝慧,高 洁.树莓叶枯病的发生及病原菌的鉴定[J].北方园艺,2013,37(17):121.
WANG Na,LU Bao-hui,GAO Jie.Occurrence of Raspberry Leaf Blight and Its Pathogen Identification[J].Northern Horticulture,2013,37(11):121.
[4]王忠忠,马茜,慕金凤,等.宁夏枸杞果实发育过程中黄酮类化合物含量的变化研究[J].北方园艺,2013,37(11):152.
WANG Zhong-zhong,MA Qian,MU Jin-feng,et al.Changes of Flavonoid Compounds Content During the Fruit Development of Lycium barbarum L.[J].Northern Horticulture,2013,37(11):152.
[5]王海新.阜新市树莓发展前景及对策[J].北方园艺,2013,37(03):184.
WANG Hai-xin.Development Prospect and Countermeasures of Raspberry in Fuxin[J].Northern Horticulture,2013,37(11):184.
[6]潘菊,杨双.双季树莓的组织培养及快速繁殖[J].北方园艺,2012,36(21):105.
PAN Ju,YANG Shuang.Tissue Culture and Rapid Propagation of Double-cropping Raspberry[J].Northern Horticulture,2012,36(11):105.
[7]丰雪,王铁良,周罕琳,等.小管出流条件下树莓灌溉施肥制度研究[J].北方园艺,2012,36(21):158.
FENG Xue,WANG Tie-liang,ZHOU Han-lin,et al.Study on Irrigation and Fertilization System of Raspberry Under the Condition of Small Tube Flow[J].Northern Horticulture,2012,36(11):158.
[8]孙兰英,吴立仁,刘金江,等.抗寒树莓新品种“绥莓1号”的选育[J].北方园艺,2013,37(18):163.
SUNLan-ying,WULi-ren,LIUJin-jiang,et al.BreedingofaNewRaspberryVarietywithColdResistance‘Suiberry1’[J].Northern Horticulture,2013,37(11):163.
[9]果朋忠.树莓日光温室栽培技术[J].北方园艺,2012,36(11):52.
GUOPeng-zhong.RaspberryCultivationTechniqueinSolarGreenhouses[J].Northern Horticulture,2012,36(11):52.
[10]杨海燕,张春红,吴文龙,等.黑莓果实发育过程中抗氧化系统变化规律研究[J].北方园艺,2014,38(16):13.
YANG Hai-yan,ZHANG Chun-hong,WU Wen-long,et al.Changes on Antioxidant System in Fruit of Rubus spp.cv ‘Arapaho’ During the Development and Ripening Stage[J].Northern Horticulture,2014,38(11):13.
备注/Memo
第一作者简介：杨国慧（1963-），女，博士，教授，现主要从事小浆果种质资源与栽培生理等研究工作。E-mail:xiaoxixeb@163.com.基金项目：黑龙江省教育厅科学技术研究资助项目（12541044）。