ZHOU Li,WANG Yuan,YE Yuanting,et al.Study on High Quality Plantlets Propagation Technique of Dendrobium officinale[J].Northern Horticulture,2016,40(10):95-99.[doi:10.11937/bfyy.201610024]
铁皮石斛优质试管苗繁育技术研究
- Title:
- Study on High Quality Plantlets Propagation Technique of Dendrobium officinale
- 文献标志码:
- A
- 摘要:
- 以野生铁皮石斛为试材,采用限根培养法,研究了铁皮石斛种子非共生萌发特性及优质试管苗繁育技术。结果表明:授粉120 d的种子达到生理成熟,能够顺利萌发;授粉140 d种子达到形态成熟,萌发率可达98.51%;低温黑暗限制保存原球茎可控制其生长;在特定容器中用限根培养基MS+0.10 mg?L-1 6-BA+0.3 mg?L-1 NAA+香蕉泥80 g?L-1 培养小苗可得到优质试管苗;把试管苗移栽到松树皮中,成活率可达97.62%,该研究为铁皮石斛产业化育苗提供了新方法。
- Abstract:
- Taking wild Dendrobium officinale as test material,using root restriction culture method,a high quality plantlets breeding technology of Dendrobium officinale was introduced by researching its characteristics of the asymbiotic seed germination.The results showed that the seeds of 120 days after pollination (DAP) reached maturity,the seeds could germinate;the seeds of 140 DAP could be morphologically mature,with a seed germination rate up to 98.51%;low temperature and darkness restriction could inhibit of protocorms growth;using medium of restrictions on root growth in a particular container could get high quality seedlings,restriction on root growth medium was MS+0.10 mg?L-1 6-BA+0.3 mg?L-1 NAA+80 g?L-1 mashed banana;and the survival rate could be up to 97.62% when the plantlets were transplanted to pine bark.Therefore,this research provided a new method for the industrial production of D.officinale
参考文献/References:
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备注/Memo
第一作者简介:周丽(1978-),女,贵州兴义人,硕士,副教授,现主要从事植物组织培养及植物资源保育与开发利用等研究工作。E-mail:zhouli@xynun.edu.cn. 基金项目:贵州省优秀科技教育人才省长专项资金资助项目(黔省专合字(2012)86号);贵州省教育厅2011年教育质量提升资助项目(黔教高发[2011]278号);贵州省教育厅教育创新团队资助项目(黔教合人才团队[2013]30号)。