ZHANG Lu,MA Qinqin,YUAN Xianghua,et al.Optimization of Liquid Fermentation of Ganoderma lucidum for Extracellular Polysaccharide Production[J].Northern Horticulture,2016,40(09):133-136.[doi:10.11937/bfyy.201609035]
金地灵芝液体发酵产胞外多糖的营养条件优化
- Title:
- Optimization of Liquid Fermentation of Ganoderma lucidum for Extracellular Polysaccharide Production
- Keywords:
- Ganoderma lucidum; ganoderan; response surface; liquid fermentation
- 文献标志码:
- A
- 摘要:
- 以金地灵芝为试材，探究了碳源、氮源及无机盐对其液态发酵产胞外多糖量的影响，通过单因素试验并运用Design Expert软件设计响应面试验优化发酵培养基的组分，以得到金地灵芝液态发酵产灵芝多糖的最优培养基配方。结果表明：优化后的最佳碳源为玉米粉，最佳氮源为蛋白胨，最佳辅助无机盐为MgSO4?7H2O，最优培养基配方为3.98%玉米粉，0.68%蛋白胨，0.21% MgSO4?7H2O，0.05% K2HPO4，0.05% KH2PO4，0.001%维生素B1。优化后胞外多糖得率提高了30.14%。
- Abstract:
- Taking Ganoderma lucidum as test material,the effect of carbon source，nitrogen source and minerals on liquid fermentation was investigated by single factor test and response surface test using Design Expert.The results showed that the optimal carbon source and nitrogen source were cornmeal and peptone，respectively.The optimal assistant inorganic salt was MgSO4?7H2O.The optimal formula of liquid fermentation medium was 3.98% corn meal，0.68% peptone，0.21% MgSO4?7H2O，0.05% K2HPO4，0.05% KH2PO4，0.001% vitamin B1.The increased output of Ganoderma lucidum extracellular polysaccharide was 30.14% more than before.
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备注/Memo
第一作者简介：张璐（1991-），女，硕士研究生，研究方向为微生物。E-mail：jove_tina@hotmail.com. 责任作者：王一丁（1963-），男，博士，教授，硕士生导师，现主要从事应用与环境微生物学等研究工作。E-mail：wangyiding@sicnu.edu.cn.