WANG Jinfu,WEN Caiyi,LIU Weicheng,et al.Screening and Identification of Biocontrol Bacterial Against Root-knot Nematode[J].Northern Horticulture,2016,40(09):121-124.[doi:10.11937/bfyy.201609032]
根结线虫生防细菌的筛选及鉴定
- Title:
- Screening and Identification of Biocontrol Bacterial Against Root-knot Nematode
- 文献标志码:
- A
- 摘要:
- 以抗病番茄根际土壤、根为分离对象，采用稀释分离方法，进行了根结线虫生防细菌的筛选及鉴定。结果表明：从番茄根际土和根内分离出47株细菌，其中，根内细菌7株，根际细菌40株。经杀根结线虫活性检测，获得2株活性强且稳定的细菌菌株W49、W21，对根结线虫的校正死亡率均超过90.0%，对卵的孵化抑制率达96.0%。通过菌落形态特征、生理生化特征观察和16S rDNA序列分析，W49和W21分别被鉴定为简单芽孢杆菌（Bacillus simplex）和内生芽孢杆菌（Bacillus endophyticus）。
- Abstract:
- Taking rhizosphere soil and roots of disease-resistant tomatoes as isolation objects，using dilution method,biocontrol bacterial against root-knot nematode were screened and identified.The results showed that 47 isolates were got among which 40 were from rhizosphere soil and 7 were from roots.Strains W49 and W21 showed nematicidal activity and egg hatching inhibition rate against Meloidogyne incogonita were over 90.0% and 96.0%,which were identified as Bacillus simplex and Bacillus endophyticus based on cultural and morphological characteristic and 16S rDNA sequencing analysis.
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备注/Memo
第一作者简介：王进福（1990-），男，硕士研究生，研究方向为植物病害生物防治。E-mail:1142966389@qq.com. 责任作者：刘霆（1975-），男，博士，副研究员，研究方向为植物线虫生物防治。E-mail:124297976@qq.com. 基金项目：农业部公益性行业专项资助项目（201103018）；北京市农林科学院青年科研基金资助项目（QNJJ201519）。