CHEN Henglei,YU Mei,LYU Changwu.The Optimization Fermentation Process of Pleurotus ferulae Polysaccharide High-yield Strain PFPH-2[J].Northern Horticulture,2016,40(08):130-132.[doi:10.11937/bfyy.201608036]
阿魏菇多糖高产菌PFPH-2的发酵过程优化研究
- Title:
- The Optimization Fermentation Process of Pleurotus ferulae Polysaccharide High-yield Strain PFPH-2
- Keywords:
- Pleurotus ferulae; polysaccharide high-yield strain; PFPH-2; fermentation process optimization
- 文献标志码:
- A
- 摘要:
- 以阿魏菇多糖高产菌PFPH-2为试验菌株，采用营养因子正交优化及发酵过程动态培养方法，研究营养因子对PFPH-2菌丝体多糖产量的影响以及动态发酵过程PFPH-2菌丝体生物量与发酵液pH值的相关性。结果表明：正交优化组合对PFPH-2菌丝体胞内多糖积累有协同效应，在最佳组合的发酵体系中PFPH-2菌丝体胞内多糖产量达到(903.73±1.23) mg/L的最高水平；发酵体系中PFPH-2菌丝体生物量与发酵液pH值有一定相关性，以发酵液pH值降到低点且趋于稳定的时间终止发酵其菌丝体生物量最大。
- Abstract:
- Taking Pleurotus polysaccharide high-yield strain PFPH-2 as experimental strain，the influence of nutrition factor on mycelial polysaccharide yield of PFPH-2 as well as the relevance of mycelium biomass of PFPH-2 with the pH value of fermented broth were studied through the method of nutrition factor orthogonal optimization and dynamic fermentation.The results showed that the orthogonal combination indicated a synergistic effect on the accumulation of mycelium polysaccharides of PFPH-2.In the optimal combination fermentation medium，the yield of mycelium polysaccharides of PFPH-2 increased to (903.73±1.23) mg/L by the end of fermentation.Mycelium biomass had a certain correlation with the pH of fermentation broth in fermentation system of PFPH-2.The mycelium biomass achieved the maximum amount when the pH values of fermentation broth down to low and tend to stabilization.
参考文献/References:
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备注/Memo
第一作者简介：陈恒雷（1979-），男，新疆沙湾人，博士，副教授，现主要从事离子束生物工程和食药用菌遗传育种与开发及天然产物研究与开发等工作。E-mail：chl@xju.edu.cn. 基金资助：新疆维吾尔自治区青年科技创新人才培养工程资助项目（2013721007）。