[1]杨泽雄,尹丽莎,张俊,等.金铁锁腋芽诱导离体培养技术研究[J].北方园艺,2016,40(08):81-84.[doi:10.11937/bfyy.201608023]
　YANG Zexiong,YIN Lisha,ZHANG Jun,et al.Study on vitro Culture Technology by Axillary Bud Induction of Psammosilene tunicoides W.C.Wu et C.Y.Wu[J].Northern Horticulture,2016,40(08):81-84.[doi:10.11937/bfyy.201608023]
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金铁锁腋芽诱导离体培养技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年08 页码: 81-84 栏目: 出版日期: 2016-04-30
Title:
Study on vitro Culture Technology by Axillary Bud Induction of Psammosilene tunicoides W.C.Wu et C.Y.Wu
作者:
杨泽雄1; 尹丽莎2; 张俊3; 唐军荣2
（1.西南林业大学团委，云南 昆明 650224；2.云南省林业高级技工学校，云南 昆明 650213；3.西南林业大学 云南省高校林木遗传改良与繁育重点实验室，云南 昆明 650224）
Author(s):
YANG Zexiong1; YIN Lisha2; ZHANG Jun3; TANG Junrong2
（1.Communism Youth League of Southwest Forestry University，Kunming，Yunnan 650224；2.Yunnan Forestry Senior Technical School，Kunming，Yunnan 650213；3.Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province,Southwest Forestry University，Kunming，Yunnan 650224）
关键词:
金铁锁; 腋芽诱导; 组织培养
Keywords:
P.tunicoides; axillary bud induction; tissue culture
DOI:
10.11937/bfyy.201608023
文献标志码:
A
摘要:
以金铁锁带叶腋的嫩茎为外植体，开展了基本培养基、腋芽诱导和增殖培养较优培养条件的筛选，并对所获得的生根苗进行了移栽练苗试验。结果表明：MS培养基适合作为金铁锁离体培养的基本培养基；金铁锁腋芽诱导培养的适宜培养基为MS+3.0 mg/L 6-BA+0.01 mg/L TDZ+0.20 mg/L NAA，平均诱导芽数为4.15；在培养基1/2MS+0.30 mg/L IBA+0.10 mg/L NAA+0.3 g/L 活性炭中进行生根培养，生根率可达89.6%；在腐殖土∶红土∶珍珠岩=1∶1∶1的基质中练苗，金铁锁组培苗的成活率可达90.5%。
Abstract:
The tender stem with leaf axil of Psammosilene tunicoides was used as explants to select the basic culture medium，the best culture condition of axillary bud induction and multiplication culture.The transplanting and exercising of rooting seedling were also be carried out.The results showed that MS culture medium was suited to use as the basic culture medium to vitro culture of P.tunicoides.The good medium for axillary bud induction of P.tunicoides was MS+3.0 mg/L 6-BA+0.01 mg/L TDZ+0.20 mg/L NAA，the average induction buds was 4.15.In rooting meduinm of 1/2MS+0.30 mg/L IBA+0.10 mg/L NAA+0.3 g/L CA，the rooting rate was 89.6%；planting in the matrix of humus∶laterite∶perlite=1∶1∶1，the survival rate of P.tunicoides was 90.5%。

参考文献/References:

 

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备注/Memo

第一作者简介：杨泽雄（1978-），男，云南洱源人，硕士，讲师，现主要从事教学管理及风景园林等工作。E-mail：ynsyxny@163.com. 基金项目：云南省高校林木遗传改良与繁育重点实验室基金资助项目。

更新日期/Last Update: 2016-06-24