SUN Yunfei,CHAO Jianguo,GU Wei,et al.Effect of High Temperature Stress on Photosynthetic Physiological Indexes and Chlorophyll Fluorescence Characteristics of Salvia miltiorrhiza Bga[J].Northern Horticulture,2016,40(06):142-147.[doi:10.11937/bfyy.201606036]
高温胁迫对丹参光合生理指标及叶绿素荧光特性的影响
- Title:
- Effect of High Temperature Stress on Photosynthetic Physiological Indexes and Chlorophyll Fluorescence Characteristics of Salvia miltiorrhiza Bga
- Keywords:
- Salvia miltiorrhiza Bga; high temperature stress; physiological characteristics; photosynthesis characteristies; chlorophyll fluorescence
- 文献标志码:
- A
- 摘要:
- 以丹参为试材，采用土培盆栽法，于第1茬花开前进行高温胁迫处理，研究了高温胁迫对丹参生理指标、光合及叶绿素荧光特性的影响。结果表明：随着高温胁迫时间的延长，丹参叶片超氧化物歧化酶（SOD）活性呈上升趋势，而过氧化氢酶（CAT）活性呈下降趋势；丙二醛（MDA）、游离脯氨酸（Pro）含量均呈上升趋势，且与胁迫时间呈极显著多项式关系。净光合速率（Pn）、气孔导度（Gs）、胞间二氧化碳浓度（Ci）和蒸腾速率（Tr）均呈下降趋势，且各指标均与胁迫时间呈负相关。最大光化学量子产量（Fv/Fm）、PSⅡ潜在光化学活性（Fv/Fo）、有效光化学量子产量（Fv′/Fm′）、光化学淬灭系数（qP）和表观光合电子传递速率（ETR）均呈下降趋势，且各指标均与胁迫时间呈负相关，非光化学淬灭系数（NPQ）呈先上升后下降趋势。研究表明，高温(40℃)对丹参生理指标、光合及叶绿素荧光特性有较大的影响，且各功能指标共同作用，以减轻植株所受伤害。
- Abstract:
- In order to explore the effect of high temperature stress on the physiological indexes，photosynthesis parameters and chlorophyll fluorescence parameters of Salvia miltiorrhiza Bga，the physiological characteristics in Salvia miltiorrhiza Bga with the soil pot experiments were measured and analyzed under high temperature stress before first flowering.The results showed that the activities of superoxide dismutase (SOD) showed a rising trend with the increasing stress time，while the activities of catalase (CAT) decreased.The malondialdehyde (MDA)，proline (Pro) content showed a rising trend，and the relationship between them and stress time was significant multiple nonlinearity.The net photosynthetic rate (Pn)，stomatal conductance (Gs)，transpiration rate (Tr) and stomatal limitation (Ls) decreased and all of them were negatively correlated with stress time.The maximum photochemical efficiency of PSⅡ (Fv/Fm)，potential photochemical efficiency (Fv/Fo)，actual photochemical efficiency of PSⅡ(Fv′/Fm′)，photochemical quenching coefficient (qP) and electron transport rate (ETR) of them decreased with rising high temperature stress time，and all of them were negatively correlated with stress time.The non-photochemical quenching coefficient (NPQ) was firstly increased and then decreased.All the analysis showed that the high temperature stress was an important adversity factor in the physiological indexes，photosynthesis parameters and chlorophyll fluorescence parameters of Salvia miltiorrhiza Bga，and the interaction of the physiological characteristics reduced the damage of the high temperature stress.
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备注/Memo
第一作者简介：孙云飞(1982-)，男，硕士，主管中药师，研究方向为中药资源培育与利用。E-mail：18901595599@163.com. 责任作者：巢建国(1960-)，男，硕士，教授，现主要从事中药资源开发等研究工作。E-mail：jgchaol016@163.com.基金项目：贵州省中药现代化科技产业研究开发专项资助项目（S2013ZP00503916360）；江苏高校优势学科建设工程资助项目（ysxk-2015）。