[1]李吉涛,谢伟玲,柴胜丰,等.三种金花茶低温半致死温度研究[J].北方园艺,2016,40(06):59-63.[doi:10.11937/bfyy.201606016]
　LI Jitao,XIE Weiling,CHAI Shengfeng,et al.Study on the Semi-lethal Low Temperature of Three Species of Camellia Sect.Chrysantha[J].Northern Horticulture,2016,40(06):59-63.[doi:10.11937/bfyy.201606016]

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三种金花茶低温半致死温度研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷期数: 2016年06 页码: 59-63 栏目: 出版日期: 2016-03-30

Title:: Study on the Semi-lethal Low Temperature of Three Species of Camellia Sect.Chrysantha

作者:: 李吉涛; 谢伟玲; 柴胜丰; 唐健民; 韦霄; （广西壮族自治区中国科学院广西植物研究所，广西桂林 541006）

Author(s):: LI Jitao; XIE Weiling; CHAI Shengfeng; TANG Jianmin; WEI Xiao; (Guangxi Institute of Botany，Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin，Guangxi 541006)

关键词:: 金花茶; 低温胁迫; 相对电导率; Logistic方程; 半致死温度(LT₅₀)

Keywords:: Camellia Sect.Chrysantha; cold stress; relative electric conductivity (REC); Logistic equation; semi-lethal temperature (LT50)

DOI:: 10.11937/bfyy.201606016

文献标志码:: A

摘要:: 以盆栽1年生毛籽金花茶、凹脉金花茶和平果金花茶3种金花茶实生苗为试材，采用人工模拟低温环境的方法对其叶片进行低温胁迫处理，测定其电解质外渗率，结合Logistic方程得出3种金花茶的低温半致死温度，并测定不同温度下叶片的游离脯氨酸、可溶性糖和丙二醛（MDA）含量。结果表明：在低温胁迫下，3种金花茶叶片相对电导率随温度降低呈“S”型上升，丙二醛、脯氨酸和可溶性糖含量均呈先上升后下降的趋势；低温半致死温度分别为-14.25、-13.25、-12.62℃，耐寒性由强到弱依次为毛籽金花茶>凹脉金花茶>平果金花茶。

Abstract:: To evaluate the cold tolerance of Camellia Sect.Chrysantha under artificial simulated low temperature environment，one-year-old potted seedlings of Camellia ptilosperma，Camellia impressinervis and Camellia pingguoensis were used as the test materials.The low temperature semi-lethal temperature (LT50) were detected with relative electric conductivity (REC) method combined with Logistic equation.The contents of free proline，soluble sugar and malondialdehyde (MDA) were also analyzed.The results showed that the REC of the three species of Camellia Sect.Chrysantha increased following an S-curve with the temperature dropped，and the contents of free proline，soluble sugar and MDA increased firstly，and then decreased.The LT50 of the three species of Camellia Sect.Chrysantha were -14.25℃,-13.25℃ and -12.62℃，respectively.The cold resistance of the three species of Camellia Sect.Chrysantha followed the order was C.ptilosperma >C.impressinervis >C.pingguoensis.

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备注/Memo

第一作者简介：李吉涛(1980-)，男，湖北襄阳人，博士，助理研究员，现主要从事植物分子生物学等研究工作。E-mail：ljtyouth@163.com. 责任作者：韦霄（1967-），男，广西天峨人，博士，研究员，现主要从事濒危植物的保护生物学等研究工作。E-mail：weixiao@gxib.cn. 基金项目：国家自然科学基金资助项目（31160137）；广西自然科学基金资助项目（2015GXNSFBA139051）；广西植物研究所基本业务费资助项目（桂植业14004）；广西林业科技资助项目（桂林科字[2012]第26号）；广西科学研究与技术开发计划资助项目(桂科重1355001-5-4)；桂林市科技攻关资助项目（20130414）；广西自然科学基金资助项目（2013GXNSFAA019081）。

更新日期/Last Update: 2016-05-03